1. Team Biological Research in Canisters BRIC-21 SpaceX-6 Dinah Dimapilis ESC Payload Project Lead Kennedy Space Center, FL

2. Team Science Experiment BRIC-21 Hardware Payload Assembly Packaging for Ascent Crew Operations Timeline Agenda

3. Team BRIC-21: Global transcriptome profiling to identify cellular stress mechanisms responsible for spaceflight-induced antibiotic resistance Dr. Wayne Nicholson (PI), University of Florida Dr. Patricia Fajardo-Cavazos (Co-PI), University of Florida Specimens: Bacillus subtilis spores and Staphlyococcus epidermidis cells (Tox 0; BSL 1) Growth Medium: Trypticase Soy Yeast (TSY) + 10% Glycerol Solution (Tox 0; BSL 1) Purpose: To understand how organisms that are normally harmless in healthy immune system could potentially be harmful to astronauts (with weaken immune system); Identify the different stress responses that happen in microgravity, leading to alternations in antibiotic susceptibility Determine the difference in growth rates Determine resistance levels to antibiotics and rates of natural changes in the cell that cause it to be unaffected by antibiotics Science Experiment

4. Team 1 Actuator Tool BRIC-21 Hardware 4 BRIC-PDFU Canisters 1 Rod Kit

5. Team Payload Assembly Petri Dish Fixation Unit (PDFU) 5 PDFUs per canister 60 mm Petri DishTemperature Data Logger 1 per canister

6. Team Ascent: Cargo Transfer Bag (Ambient) Packaging for Ascent BRIC-PDFU Canisters Actuator Tool

7. Team Crew Operations Unpack from CTB and actuate using Actuator Tool and Rod Actuate with clip installed

8. Team Crew Operations BRIC-21 requires only a partial actuation Rod drives growth medium to specimen Upon actuation, specimens enter their growth period

9. Team Timeline