1. Genetic Technology Chapter 13

2. Foldable Fold your paper so both sides meet in the middle. Cut 3 flaps on each side for a total of 6 flaps. Do this with 2 sheets of paper. Label the sides of one paper: inbreeding, Clone, genetic engineering, plasmid, recombinant DNA, restriction enzyme. Label the side of the other paper: transgenic organism, vector, gene therapy, human genome, PCR

3. Inbreeding Under the flap for inbreeding type the following definition and give an example Mating closely related individuals to ensure they are homozygous for most traits Can be bad because it may bring out harmful recessive traits Example: Pure bred Dogs

4. Cloning Write the following definition under the flap and give examples Genetically identical copy of an organism Used in Bacteria to make insulin, also used in plants.

5. Genetic Engineering Under the genetic engineering flap write the following definitions and examples Method of cutting DNA from one organism and inserting the DNA fragments into a host organism of the same or different species. Scientist use Ecoli bacteria to make expensive Die for blue Jeans

6. Plasmid Under the plasmid flap write the following definition and sketch the following picture. A small round band of DNA found in bacterial cells that is used as a biological vector (carrier) http://lc- molecular.wikispaces.com/file/view/cnighting ale_plasmid.jpg/145295529/cnightingale_plas mid.jpg http://lc- molecular.wikispaces.com/file/view/cnighting ale_plasmid.jpg/145295529/cnightingale_plas mid.jpg

7. Recombinant DNA Under the flap write the following definition and examples DNA made by recombining (connecting) fragments of DNA from different sources. Example combining the DNA for the protein that makes blood clot and putting it into sheep DNA to make massive amounts of the clotting factor

8. Restriction Enzyme Under the flap for restriction enzyme write the following definition and examples A DNA cutting enzyme that can cut bothe strand of a DNA molecule at a specific nucleotides site. When making Recombinant DNA the enzyme separates the DNA at the right spot so it can be connected to the foreign DNA

9. Foldable 2

10. Transgenic organism Write the following definition and example Organism that contains DNA from 2 different species. Ex Bacteria that have had human DNA put into them to make human insulin for Diabetics Plants that have had DNA from other species in them to prevent rotting or make them bigger and more desirable.

11. Vector Write the following definition and examples under the flap; Means by which DNA from another species can be carried into the host cell. May be biological (living organism) or Mechanical (machine in the lab) Viruses and bacteria

12. Gene therapy Under the flap write the following definition and examples Insertion of normal genes into a human cell to correct genetic disorders Used to treat: Cystic fibrosis, sickle-cell anemia and hemophilia

13. Human Genome Under the Human Genome flap write the following definition and examples Map of the thousands of genes on 46 human chromosomes that when mapped and sequenced may provide information on the treatment and cure of genetic disorders There are approximately 35,000 to 40,000 genes on the 46 human chromosomes

14. PCR Under the PCR flap write the following definition and examples. Polymerase chain reaction A machine that makes millions of copies of DNA in a day. Used to analyze DNA in crime investigations and in the diagnosis of diseases

15. Applications of DNA technology On the last flap title it application of DNA technology. Under the flap write the following examples: Develop corn that contains as much protein as beef, make insulin and human growth hormone, increase vitamins in crops, make plants produce toxins that make them resistant to bacteria and insects