1. MOL The Mission Operations Laboratory MOL The Mission Operations Laboratory NASA MSFC Huntsville, Alabama Cell Bio Tech Demo TIR Overview Aug 28, 2012 Tony Cox EO20 Payload Ops Lead 256-961-0039 tony.w.cox@nasa.gov

2. MOL The Mission Operations Laboratory Page 2 NASA MSFC Huntsville, Alabama Cell Bio Tech Demo Hardware

3. MOL The Mission Operations Laboratory Page 3 NASA MSFC Huntsville, Alabama General Information  The Cell Bio Tech Demo consists of four unique experiments which are being used to develop the next generation of incubator for space life science research. The experiments will run in triplicate and are:  Medium Tray 1 and Medium Tray 2  Opticell Interface Tray  Specimen Transfer Tray  Hardware Configuration for ascent:  Both CTBs launch and stow at ambient as well as for return. However the three yeast cultures from the Specimen Transfer Tray will return in a +4°C DCB Media Tray 1 Kit (3) Media Tray 2 Kit (3) OptiCell Interface Tray Kit (1) Tubing Cutter Specimen Transfer Tray Kit (3) Tubing Cutter Cell Bio Tech Demo CTB 1 (L-4 week turnover) Cell Bio Tech Demo CTB 2 (L-48 hrs turnover)

4. MOL The Mission Operations Laboratory Page 4 NASA MSFC Huntsville, Alabama 4 Medium Tray 1 & 2 Media Bag 1 Media Bag 2 Syringe Invision Connector Crimp Tubing Media Bag Crimp Tubing Nutrient Transfer Tool Media Transfer Container Velcro Tab Media Transfer Tray 1 (3 ea): Ops: Transfer 5 ml of Tryptic Soy Broth (TSB) from Media Transfer Container to Media Bag containing TSB using the syringe-based Sample Tool to simulate starting a cell culture Demo: Maintenance of medium sterility using crimp tool to close tubing Media Transfer Tray 2 (3 ea): Ops: Transfer 10 ml of TSB between Media Bags pre-filled with TSB to simulate specimen transfer, such as subculturing Demo: Maintenance of medium sterility

5. MOL The Mission Operations Laboratory Page 5 NASA MSFC Huntsville, Alabama 5 Opticell Interface and Specimen Transfer Bead Bag OptiCell™ Sampling Tool OptiCell™ YPD Bag Crimp Tubing Yeast Sample Tool YeastTransfer Container Velcro Tab OptiCell™ Interface Tray (1 Tray, 3 OptiCell™s) : Ops: Transfer fluorescent beads (in phosphate buffered saline (PBS) into an Opticell™ filled with PBS Demo: Use of the Sample Tool for transferring a specimen to a carrier that can be moved to another ISS analysis facility Specimen Transfer Tray (3 ea): Ops: Inoculate a live S. cerevisiae (yeast in stasis in saline) to a medium bag pre- filled with YPD medium using the Sample Tool Demo: Use of Sample Tool for transferring a live yeast to initiate culture growth; Education Outreach element

6. MOL The Mission Operations Laboratory Page 6 NASA MSFC Huntsville, Alabama Operations  Crew Training will consists of a short video just prior to each experiment.  Medium Tray 1 and Medium Tray 2 are two separate experiments but will be conducted back to back.  Three trays for each experiment  Opticell Interface Tray will utilize only one tray but has three Opticell cards.  Specimen Transfer Tray (separate CTB) has three trays each containing a YPD bag which will be removed from tray and allowed to incubate at ambient upon completion of experiment. After incubation the YPD Stowage Bag will be inserted into MELFI at 4°C.  There is a questionnaire at the end of each experiment.

7. MOL The Mission Operations Laboratory Page 7 NASA MSFC Huntsville, Alabama Safety Summary  The Safety Data Pack …….has not yet been signed. However, there are no expected hazards for this experiment.  Tox level is zero for all trays.