1. DO NOW 1. Get out your Embryos-R-Us HW 2. Detail the decision you made. Why did you make that decision?

2. What is Gel Electrophoresis? Process used to separate fragments of DNA using a charge

3. 1 st Step in Electrophoresis Prepare the gel Need agarose, mold, and comb

4. 2 nd Step in Electrophoresis Prepare the box Need electrophoresis chamber and buffer

5. 3 rd Step in Electrophoresis Load DNA into the wells of the gel using a micropipette DNA Well

6. 4 th Step in Electrophoresis Run electricity to separate DNA fragments

7. 5 th Step in Electrophoresis Stain the gel to see the DNA fragments Analyze results

9. What is the Result? Well DNA Fragment Shorter Fragments Larger Fragments ___ +

10. How to read a gel? Start by reviewing paternity bands (from Mom and Dad) Larger Smaller Sex D1 = Real Daughter D2 = Daughter of Dad S1 = Real Son S2 = Adopted Son

11. EXAMPLE I: WHO IS THE CRIMINAL?

12. EXAMPLE II: WHO IS THE CRIMINAL?

13. Example III: WHO IS THE CRIMINAL?