3. Final Project Visit the Lab Research Meetings with Advisor

4. Primitive Cells Self- renewal Originate differentiated cells and tissues

5. Potential TotipotentPluripotent Embryonic Stem Cells Multipotent Adult Stem Cells

6. Cellular Therapy - Replacement of damaged tissues or cells Bone Marrow transplantation Gene therapy Fundamental research Pharmaceutical research Therapeutical Applications

7. AdvantagesDisadvantages Limited proliferative capacity Limited Differentiation Potential Free from Ethical Issues Safety AdvantagesDisadvantages Safety Issues Ethical Issues Higher proliferative capacity Pluripotent

8. What are they? Where are they? Are they abundant? Adult Stem Cells (Multipotent) Bone Marrow, Umbilical Cord, Adipose Tissue, etc… No 0,01%-0,001% of mononuclear cells from Adult Bone Marrow

9. Which cells can they originate?

10. Why are they special? Efficient Isolation and Amplification in culture without loosing capacities Possess Immunomodulatory properties and are Not rejected by the immune system ( allogeneic settings)

11. Cellular Therapy Clinically Relevant Cell Numbers Isolation of MSC from Bone Marrow Efficient isolation and expansion Most Pure Adipose Tissue Less invasive procedure Umbilical Cord Non-invasive procedure Isolation by a density gradient How do scientists isolate MSC?

12. How do scientists expand MSC? Requirements Clean Room Weekly passage (to keep the potential) Minimize the risks of karyotypic changes Novel Approaches Bioreactors Serum-free, Xeno-free components Hypoxic Atmosphere GMP-produced basic medium LP02

13. Applications Prevent graft rejection and graft-versus- host disease in Bone Marrow Transplantation settings (immunomodulatory properties) Source of cells for Tissue Engineering Applications Gene Delivery Approaches Others (Potentially prevent rejection in solid organ transplantation )

14. Future applications of MSC Optimization of Culture Processes for MSC

15. http://www.bigmae.com/tudo-sobre-celulas-estaminais/ http://docs.google.com/gview?a=v&q=cache:pf3clGZ6f08J:www.cn ecv.gov.pt/NR/rdonlyres/8D379340-EDC6-468F-9C77- D5165652D8B3/0/P047_DeclaracoesCE.pdf+celulas+estaminais&hl =pt-BR&sig=AFQjCNENCyrHRxUGR4j60Mh70MhM3Bn4tA http://www.acidigital.com/estaminais.htm http://www.medinfar.pt/medinfaronline/pt/grupo/biotecnologia/cyt otherababy/celulasestaminaishttp://www.medinfar.pt/medinfaronline/pt/grupo/biotecnologia/cyt otherababy/celulasestaminais/ http://www.criovida.pt/celulas.html http://www.vatican.va/roman_curia/pontifical_academies/acdlife/d ocuments/rc_pa_acdlife_doc_20000824_cellule-staminali_po.html http://celulas-estaminais.blogs.sapo.pt/1016.html http://www.bioteca.pt/glossario.asp http://biblioteca.universia.net/html_bura/ficha/params/id/38072812.html http://www.scielo.br/pdf/aob/v14n1/a04v14n1.pdf http://www.tribunamedicapress.pt/internacional-1/19389-celulas- estaminais-matam-celulas-cancerigenas http://medicosdeportugal.saude.sapo.pt/action/2/cnt_id/2869/ http://www.crioestaminal.pt/pt/cp/pidcrioestaminal.aspx http://www.pcd.pt/noticias/ver.php?id=7910 http://www.josedemello.pt/gjm_press_05.asp?lang=pt&empresa=2 &noticia=8139 http://www.crioestaminal.pt/pt/pdfs/Artigo_20MAC.pdf http://medicosdeportugal.saude.sapo.pt/action/2/cnt_id/2953/ http://en.wikipedia.org/wiki/Mesenchymal_stem_cell http://www.isscr.org/public/adultstemcells.htm http://www.rndsystems.com/molecule_group.aspx?g=811&r=7 http://www.mayoclinicproceedings.com/content/84/10/859.full http://www.stemcellpatents.com/news-show-385 http://www.medscape.com/viewarticle/586273 http://arthritis-research.com/content/10/5/223 http://www.haematologica.org/cgi/content/abstract/94/2/173 http://www.stemcell.com/technical/29018_Mesenchymal.pdf http://www.medicalnewstoday.com/articles/112419.php http://www.freshpatents.com/Method-for-distinguishing- mesenchymal-stem-cell-using-molecular-marker-and-use-thereof- dt20090917ptan20090232773.php http://www.tribunamedicapress.pt/internacional-1/15367-celulas- estaminais-poderao-tratar-esclerose-multipla http://celulas-estaminais.blogs.sapo.pt/tag/hematopoi%C3%A9ticas http://biotecnologiaportugal.wordpress.com/2009/06/23/celulas- estaminais-tem-um-elevado-potencial-para-biotecnologia-da-saude/ http://www.bebevida.com/CeluAplicacoes.asp http://en.wikipedia.org/wiki/Hematopoietic_stem_cell http://stemcells.nih.gov/info/scireport/chapter5.asp http://emedicine.medscape.com/article/991032-overview http://ocw.mit.edu/NR/rdonlyres/Biology/7-012Fall-2004/E8A49E3B- F92A-4A6D-A439-FDF76DDC273F/0/hematopoiesis_st.pdf http://emedicine.medscape.com/article/208954-overview http://www.isscr.org/public/selfrenewal.htm http://www.isscr.org/public/rejection.htm http://www.worldscibooks.com/etextbook/5729/5729_chap1.pdf http://www.liebertonline.com/doi/abs/10.1089/scd.2004.13.436?cookie Set=1&journalCode=scd http://www.rndsystems.com/molecule_group.aspx?g=805&r=7 Links (consulted between 09.10.09 and 31.10.09)

16. Stem Cells: Scientific Progress and Future Research Directions, June 2001 Monography: Mesenchymal Stem Cells and Immunomodulation Aplications, GALANTE, João N. R., 2008/2009 Books and Articles Images (consulted between 09.10.09 and 31.10.09) http://linoresende.jor.br/wp-content/uploads/2009/05/pergunta.jpg http://richardwiseman.files.wordpress.com/2009/05/question-mark3a.jpg http://www.legis.state.wi.us/senate/sen11/news/images/questions.jpg http://imgs.sfgate.com/blogs/images/sfgate/ybenjamin/2009/09/02/question-cloud.jpg http://razorland55.free.fr/friend01/Question_Mark2.jpg http://trueslant.com/katiedrummond/files/2009/05/stem-cell-harvest.jpg http://student.biology.arizona.edu/honors2007/group01/embryonic-stem-cell.jpg http://genomicenterprise.com/yahoo_site_admin/assets/images/stem_cell.358142642.jpg http://futuresteve.files.wordpress.com/2007/11/embryonic-stem-cell-6660.jpg http://repairstemcell.files.wordpress.com/2009/02/stem_cell.jpg http://msnbcmedia1.msn.com/j/msnbc/Components/Photos/060404/060404_bladder_hmed_7a.hmedium.jpg http://www.northengland.com/images/side_images/stemcells%20LiSci%20regen.jpg http://stemcelldoc.files.wordpress.com/2009/02/msc-srtem-cell2.jpg http://www.abcam.com/ps/datasheet/images/ab616_4.jpg http://www.chirurgische-klinik.de/forschung/experimed/pic/3D_O2.jpg http://www.journal-inflammation.com/content/figures/1476-9255-2-8-1.jpg http://celleng-tech.com/index/hamsc.jpg http://www.stemcelltherapy21.com/stemcelltherapy21/stemcells/images/mesenchymal%20stem%20cell.jpg http://www.ms-cartpro.de/uploads/pics/abb3_01.jpg http://www.rochester.edu/pr/Review/V69N1/images/feaStemCellImage.jpg http://3.bp.blogspot.com/_zaUriuGy1tE/Rc6Ag0FjUKI/AAAAAAAAAD8/SMrlmY7rfrU/s400/zigoto1.jpg

17. 67312 Andreia Santos 67316 Catarina Caneira 67346 Teresa Rodrigues 1st Year, 1st Semester 2009/2010 Introduction to Biomedical Engeenier