1. Comparison of the Varian 920-LC with Agilent 1200 Series HPLC

2. 2 Varian 920-LC Market Competitors Competitors Shimadzu Waters Agilent Dionex The 920-LC presents a significant step forward for the Varian

3. Varian 920-LC Compared to the Agilent 1200 Series LC

4. 4 Agilent 1200 Quaternary Pump G1354A Dual piston, low pressure quaternary gradient pump Electromechanical check valve Variable piston stroke Pulse damper between primary and secondary piston Upgrade?? of 1100 series Requires degasser module

5. 5 Agilent 1200 Autosamplers Option of G1329A standard Autosampler capable of 100 HPLC vials G1367B Enhanced Autosampler compatible with either 2 microplates or 108 HPLC vials Standard carryover <0.1% but can be improved to <0.01% using needle wash Both have peltier cooled option

6. 6 Agilent 1200 Detectors Wide range of detectors Variable Wavelength Detector Multi-Wavelength Detectors Diode Array Detector Fluorescence Detector RI Detector

7. 7 UV-Vis Detectors Sensitivity Comparison Typical Agilent VWD S/N ~ 25 Typical Varian 920-LC UV/Vis S/N > 36

8. 8 Varian 920-LC Compared to Agilent 1200 Agilent 1200Varian 920-LC Pulse DamperYesNot Required Microplate compatibility Not CompatibleUp to 3 96 well plates Autosampler Capacity 100 HPLC vials200 HPLC vials Dual UV-Vis Capability NAYes Carryover<0.1%<0.01% UV-Vis range 190 – 600 D2 only190 – 900 D2 & QI UV detector noise+ 0.75 x 10 -5 Au+ 0.5 x 10 -5 Au

9. 9 Agilent 1200 Purification Systems Agilent offer 2 systems for purification Analytical scale < 10 ml/min Prep scale for < 100 ml/min

10. 10 Agilent 1200 Purification Systems Agilent claim modular approach for maximum flexibility But there is practically no overlap between AS system and PS system Require different pumps, autosamplers, fraction collectors, flow cells even software!!

11. 11 Agilent 1200 Detectors Agilent do not offer a analytical to prep solution Requires two systems to operate at 4.6 mm and 21.2 mm ID columns No dual path flow cells Require up to 3 different flow cells to perform scale up work from analytical scale to prep scale PS pump flow rate range 0.5 to 100 ml/min PS autosampler uses dual fixed loops Limited injection volume ranges Do not offer through pump injector

12. Questions??