4. https://thecrowuwb.wordpress.com/

5. Living up to your Nernst potential: a graphical approach to electrochemical gradients Gregory J. Crowther, Ph.D. –∞ +∞ Membrane potential Net driving force OUT IN Tues., Feb. 9 2:30pm Discovery 464

6. Learn about coral microbiomes from Jesse Zaneveld Student lunch : Thursday, February 11 th at 12:00 pm. Email hilleskl@uw.edu for details.hilleskl@uw.edu Research Talk : Thursday, February 11 th at 2:00 pm in UW2-307 Title: Exploring the Microbial Wilderness: Theory, Tools, and Applications from Chronic Disease to Wildlife Conservation Teaching demonstration : Friday, February 12 th at 11:00am in UWBB 205 Title: Interactive Demo: Key Perspectives on Microbial Communities in Health and Disease Photo from: http://oregonstate.edu/microbiology/vegathurberlab/feature-stories/saudi-arabia-2015

7. David Sommerfeld, Ph.D.

8. BBio 351 – February 1, 2016 Today’s outline Finish endocrinology (adrenal disorders, thyroid paper) Start Chapter 16: Reproduction! More endocrinology, really Return of the hypothalamic-pituitary axis H. Hasuwa et al., Science 2013 Examples of / exceptions to negative feedback Ovarian/uterine cycle LH surge Birth control

9. Reproduction, homeostasis, negative feedback

10. Reproduction: connection with endocrinology F. H. Martini et al. (2014)

11. Gamete and embryo development Sherwood Figures 16-3 and 16-4c

12. Female (cat) reproductive anatomy biology.clc.uc.edu/fankhauser/

13. Male reproductive anatomy CAT biology.clc.uc.edu/fankhauser/ SHEEP fau.pearlashes.com/anatomy/ dissections/reproductive/testis10.jpg

14. The ovarian & uterine cycles 10 th Martini Figure 28-24 (like Sherwood Figure 16-16) Day 0 Day 14 Day 28

15. The ovarian & uterine cycles 10 th Martini Figure 28-24 Day 0 Day 14 Day 28

16. 10 th Martini Figure 28-24 (like Sherwood Figure 16-17)

17. What’s “wrong” with this picture? Sherwood Figure 16-18a

18. Tracking the cycle to promote or avoid pregnancy Urine test – which hormone should be tracked?

19. Birth control: “the pill” What does it contain? How is pregnancy avoided? Prevent ovulation? Prevent fertilization? Prevent implantation? Picture: JournalTimes.com

20. faculty.washington.edu/crowther/Misc/Songs/

21. New research on the hypothalamic-pituitary-gonadal axis: H. Hasuwa et al., Science 341: 71-73, 2013 Title: “miR200b and miR429 function in mouse ovulation and are essential for female fertility” Review: hypothalamic-pituitary-gonadal axis Main question: exactly does GnRH promote LH secretion? Review: control of gene expression

22. What are microRNAs (miRNAs)? Stefanie S. Jeffrey, Nature Biotechnology 2008

23. Genetic tools/tricks: reporter assay to see how miR-200b & miR-429 affect Zeb1 expression Hasuwa et al. (2013), Figure S13

24. Genetic tools/tricks: overexpressing Zeb Google Images search results; Hasuwa et al. (2013), Figure 3B-C

25. Literature-reading tip: figure (and table) titles often collectively tell the story! Fig. 1. Infertility phenotype of miR-DKO mice. Fig. 2. Pituitary defects in miR-DKO female mice. Fig. 3. Lhb expression is regulated by ZEB1 as a potential target of miR-200b and miR-429.

26. Fig. 1. Infertility phenotype of miR-DKO mice. Hasuwa et al. (2013), Figure 1A

27. Fig. 1. Infertility phenotype of miR-DKO mice. Hasuwa et al. (2013), Figure 1C-F

28. Fig. 2. Pituitary defects in miR-DKO mice. Hasuwa et al. (2013), Figure 2A-D

29. Fig. 2. Pituitary defects in miR-DKO mice. Hasuwa et al. (2013), Figure 2E-H

30. Fig. 3. Lhb expression is regulated by ZEB1 as a potential target of miR-200b and miR-429. Hasuwa et al. (2013), Figure 3D-E

31. Diagram to summarize main findings?

32. What questions could be researched next?