1. End Show Slide 1 of 31 Copyright Pearson Prentice Hall Biology

2. End Show Slide 2 of 31 Copyright Pearson Prentice Hall 1-4 Tools and Procedures

3. End Show Slide 3 of 31 Copyright Pearson Prentice Hall 1-4 Tools and Procedures Learning Objectives 1.4.1 Describe the measurement system most scientist use. 1.4.2 Explain how light microscopes and electron microscopes are similar and different

4. End Show 1-4 Tools and Procedures Slide 4 of 31 Copyright Pearson Prentice Hall A Common Measurement System What measurement system do most scientists use?

5. End Show 1-4 Tools and Procedures Slide 5 of 31 Copyright Pearson Prentice Hall A Common Measurement System Scientists need a common system of measurements in order to replicate each other’s experiments.

6. End Show 1-4 Tools and Procedures Slide 6 of 31 Copyright Pearson Prentice Hall A Common Measurement System Most scientists use the metric system when collecting data and performing experiments.

7. End Show 1-4 Tools and Procedures Slide 7 of 31 Copyright Pearson Prentice Hall A Common Measurement System The metric system is a decimal system of measurement whose units are based on certain physical standards and are scaled on multiples of 10. Because the metric system is based on multiples of 10, it is easy to use.

8. End Show 1-4 Tools and Procedures Slide 8 of 31 Copyright Pearson Prentice Hall A Common Measurement System A revised version of the original metric system is called the International System of Units, or SI.

9. End Show 1-4 Tools and Procedures Slide 9 of 31 Copyright Pearson Prentice Hall A Common Measurement System 1 meter (m) = 100 centimeters (cm) 1 meter = 1000 millimeters (mm) 1000 meters = 1 kilometer (km) 1 kilogram (kg) = 1000 grams (g) 1 gram = 1000 milligrams (mg) 1000 kilograms = 1 metric ton (t) 0 0 C = freezing point of water 100 0 C = boiling point of water 1 liter (L) = 1000 milliliters (mL) 1 liter = 1000 cubic centimeters (cm 3 )

10. End Show 1-4 Tools and Procedures Slide 10 of 31 Copyright Pearson Prentice Hall 1 2 3 4 5 6

11. End Show 1-4 Tools and Procedures Slide 11 of 31 Copyright Pearson Prentice Hall Analyzing Biological Data Scientists collect data to find out whether certain factors change or remain the same. Often, the simplest way to do that is to record the data in a table and then make a graph.

12. End Show 1-4 Tools and Procedures Slide 12 of 31 Copyright Pearson Prentice Hall Analyzing Biological Data Computers help scientists to gather, analyze, and present large quantities of data. Analyses of data are used to make predictions about complex phenomena.

13. End Show 1-4 Tools and Procedures Slide 13 of 31 Copyright Pearson Prentice Hall Microscopes How are light microscopes and electron microscopes similar? How are they different?

14. End Show 1-4 Tools and Procedures Slide 14 of 31 Copyright Pearson Prentice Hall Microscopes Microscopes are devices that produce magnified images of structures that are too small to see with the unaided eye.

15. End Show 1-4 Tools and Procedures Slide 15 of 31 Copyright Pearson Prentice Hall Microscopes Light microscopes produce magnified images by focusing visible light rays. Electron microscopes produce magnified images by focusing beams of electrons.

16. End Show 1-4 Tools and Procedures Slide 16 of 31 Copyright Pearson Prentice Hall Microscopes Light Microscopes The most commonly used microscope is the light microscope. Light microscopes produce clear images of objects at a magnification of about 1000 times.

17. End Show 1-4 Tools and Procedures Slide 17 of 31 Copyright Pearson Prentice Hall Microscopes Compound light microscopes allow light to pass through the specimen and use two lenses to form an image. Light microscopes make it possible to study dead organisms and their parts, and to observe some small organisms and cells while they are still alive.

18. End Show 1-4 Tools and Procedures Slide 18 of 31 Copyright Pearson Prentice Hall Microscopes Electron Microscopes Light microscopes cannot produce clear images of objects smaller than 0.2 micrometers, or about one-fiftieth the diameter of a typical cell. To study even smaller objects, scientists use electron microscopes.

19. End Show 1-4 Tools and Procedures Slide 19 of 31 Copyright Pearson Prentice Hall Microscopes Electron microscopes use beams of electrons, rather than light, to produce images. The best electron microscopes can produce images almost 1000 times more detailed than light microscopes can.

20. End Show 1-4 Tools and Procedures Slide 20 of 31 Copyright Pearson Prentice Hall Learning Objectives: 1.4.3 Describe two common lab. techniques 1.4.4 Explain why it is important to work safely in biology

21. End Show 1-4 Tools and Procedures Slide 21 of 31 Copyright Pearson Prentice Hall Laboratory Techniques Cell Cultures To obtain enough material to study, biologists sometimes place a single cell into a dish containing a nutrient solution. The cell is able to reproduce so that a group of cells, called a cell culture, develops from the single original cell.

22. End Show 1-4 Tools and Procedures Slide 22 of 31 Copyright Pearson Prentice Hall Laboratory Techniques Cell cultures can be used to: test cell responses under controlled conditions study interactions between cells select specific cells for further study

23. End Show 1-4 Tools and Procedures Slide 23 of 31 Copyright Pearson Prentice Hall Laboratory Techniques Cell Fractionation Biologists often use a technique known as cell fractionation to separate the different cell parts.

24. End Show 1-4 Tools and Procedures Slide 24 of 31 Copyright Pearson Prentice Hall Laboratory Techniques Cells are broken into pieces in a special blender. The broken cell bits are added to a liquid and placed in a tube. The tube is inserted into a centrifuge that spins, causing the cell parts to separate, with the most dense parts settling near the bottom of the tube.

25. End Show 1-4 Tools and Procedures Slide 25 of 31 Copyright Pearson Prentice Hall Laboratory Techniques A biologist can then remove the specific part of the cell to be studied by selecting the appropriate layer.

26. End Show 1-4 Tools and Procedures Slide 26 of 31 Copyright Pearson Prentice Hall Working Safely in Biology Scientists working in a laboratory or in the field are trained to use safe procedures. Laboratory work may involve contact with dangerous materials or organisms.

27. End Show 1-4 Tools and Procedures Slide 27 of 31 Copyright Pearson Prentice Hall Working Safely in Biology Follow safe practices. Study the safety rules. Read all the steps and safety precautions. Follow your teacher’s instructions and textbook directions exactly. If in doubt, ask your teacher for an explanation. Wash your hands thoroughly with soap and warm water after every scientific activity.

28. End Show 1-4 Tools and Procedures Slide 28 of 31 Copyright Pearson Prentice Hall Working Safely in Biology Remember you are responsible for your own safety as well as that of your teacher, classmates, and any live animals you handle.

29. End Show - or - Continue to: Click to Launch: Slide 29 of 31 Copyright Pearson Prentice Hall 1-4

30. End Show Slide 30 of 31 Copyright Pearson Prentice Hall 1-4 A single measurement system is commonly used in science because a.it allows scientists to easily replicate one another’s experiments. b.basic units of mass, length, and volume are unrelated to one another. c.more kinds of measurements can be made. d.computers can store large amounts of scientific data.

31. End Show Slide 31 of 31 Copyright Pearson Prentice Hall 1-4 Compared to a light microscope, an electron microscope is used to observe a.larger objects with less detail. b.larger objects with more detail. c.smaller objects with more detail. d.smaller objects with less detail.

32. End Show Slide 32 of 31 Copyright Pearson Prentice Hall 1-4 A device that separates cell parts is a a.centrifuge. b.cell culture. c.light microscope. d.electron microscope.

33. End Show Slide 33 of 31 Copyright Pearson Prentice Hall 1-4 A technique in which cells are grown in a nutrient solution is known as a.staining. b.cell fractionation. c.cell culturing. d.cell fertilizing.

34. End Show Slide 34 of 31 Copyright Pearson Prentice Hall 1-4 When you work in a biology laboratory situation, your first priority should be to a.make sure all materials are available. b.modify any instructions that do not make sense. c.familiarize yourself with all safety rules before beginning to work. d.know ahead of time what kinds of results to expect.

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