1. Do-now: Discuss and answer the following scenario o It's your senior year and you are the only one of your friends to own a car. You go away for two months (on vacation) and lend your car to a few of your friends. When you come back, your car smells and you discover a maggot infested sandwich under the front seat. All of your friends deny that it belongs to them. If you know which days each friend had the car, can you determine the identity of the culprit?

2. Chapter 12 Forensic Entomology © 2012 Cengage Learning. All Rights Reserved

3. * Forensic Science II: Forensic Entomology, Chapter 12 © 2012 Cengage Learning. All Rights Reserved Introduction—Objectives 1. Define forensic entomology. 2. Describe the anatomy of an arthropod. 3. Discuss the life cycle of insects. 4. Estimate time of death using insect evidence. 5. Examine the effects of insects on human remains.

4. * Forensic Science II: Forensic Entomology, Chapter 12 © 2012 Cengage Learning. All Rights Reserved Introduction—Objectives 6. Evaluate the use of entomological evidence to solve crimes. 7. Describe the impact of weather on metamorphosis. 8. Demonstrate proper procedures for collection and preservation of entomological evidence.

5. * Forensic Science II: Forensic Entomology, Chapter 12 © 2012 Cengage Learning. All Rights Reserved Introduction—Vocabulary o Arthropod—a phylum of animals with jointed appendages and an exoskeleton (from the Greek arthros–jointed; podes–feet) o chitin—a tough polysaccharide; the major component of an arthropod’s exoskeleton o exoskeleton—a rigid external structure made of chitin and protein (protects, provides a point of attachment for muscles; prevents water loss) o forensic entomology—the study of insects in legal situations

6. * Forensic Science II: Forensic Entomology, Chapter 12 © 2012 Cengage Learning. All Rights Reserved Introduction—Vocabulary o invertebrate - organism lacking a backbone o larva (larvae, pl) - immature, feeding stage of insects that undergo complete metamorphosis; the stage between the egg and pupa o maggot - legless larva o metamorphosis - the changes an organism undergoes as it develops into an adult o pupa (pupae, pl) - nonfeeding and relatively inactive developmental stage of some insects

7. Bugs don’t lie o https://www.youtube.com/watch?v=TqKq6nY K9mA https://www.youtube.com/watch?v=TqKq6nY K9mA * Forensic Science II: Forensic Entomology, Chapter 12 © 2012 Cengage Learning. All Rights Reserved

8. * Forensic Science II: Forensic Entomology, Chapter 12 © 2012 Cengage Learning. All Rights Reserved Bugs Don’t Lie o July 9, 1997 Champaign County, Ohio o Stepfather, Kevin Neal, claims stepchildren are missing from their back yard o Bodies discovered on September 6th were in the late stages of decomposition o Autopsies revealed Maggot infestation Absence of blowflies and screwworms Presence of cheese skipper fly in its 3rd instar stage o How were these insects evaluated?

9. 9 Insects belong to the Phylum arthropoda. Insects are invertebrates, possessing no backbone. Instead of internal structures, posess, exoskeletons made of chitin. On Insects

10. * Forensic Science II: Forensic Entomology, Chapter 12 © 2012 Cengage Learning. All Rights Reserved Introduction (Obj 12.1, 12.2) o Arthropod—an invertebrate

11. * Forensic Science II: Forensic Entomology, Chapter 12 © 2012 Cengage Learning. All Rights Reserved History

12. More than 100,000 species of insects are found almost everywhere in North America, but very few are harmful. Insects are important to the food chain, pollination, honey, wax, shellac, silk, food, scavenging, and decomposing. Wheel Bug

13. o Kingdom o Phylum o Class o Order o Family o Genus o species In school we learned that animals are divided into smaller and smaller groups. Let's look where insects fit in the animal kingdom. From top to bottom, each category has fewer species, and the groups of animals within each category are increasingly similar.

14. o Kingdom- animal o Phylum - arthropod o Class - insect o Order - diptera o Family - muscidae o Genus - Musca o species - domestica Using the house fly as an example. Notice the genus and species is the official scientific name of the animal. This name is valid in any country of the world and is an important way to avoid confusion. This two-word Latin naming system was developed in 1758 and has hardly changed since then. There are some important things to know about it.

15. House Fly Musca = fly domestica = home Scientific names are always two words. The first part of the name (Genus) is always capitalized. This lets us know that it is the genus. The second name is always in lower case and is usually descriptive of the insect in some manner. Because these words are in Latin, they are always italicized (or underlined which substitutes for italics).

16. Insects also have common names. One problem with common names is that there may be more than one common name for the same insect. Common names often differ between geographical regions. Do you know what a skeeter hawk is? Or a cow killer? Did you know a velvet ant really is not an ant, but a wingless wasp?...and locusts are really a type of grasshopper - not a cicada.

17. Skeeter Hawk Cow Killer Velvet ant Cicada Locust Locust

18. * Forensic Science II: Forensic Entomology, Chapter 12 © 2012 Cengage Learning. All Rights Reserved Insects & Forensics (Obj 12.2)

19. * Forensic Science II: Forensic Entomology, Chapter 12 © 2012 Cengage Learning. All Rights Reserved Insects o 29 orders, or related groups, of insects o 2 orders are important to forensics Flies (Diptera) Beetles (Coleoptera)

20. * Forensic Science II: Forensic Entomology, Chapter 12 © 2012 Cengage Learning. All Rights Reserved Insects at Work (Obj 12.3, 12.5) o Metamorphosis—the maturation process for insects) Eggs Larvae Pupa Adult

21. 21 Let's go outside!

22. DO-now o Read p. 348-349 o Define “Post-mortem Interval” in your notes. o Underneath, answer the following question: What evidence are you looking for when you find a body more than 72 hours after death? 22

23. Objectives 1. Estimate time of death using insect evidence. 2. Examine the effects of insects on human remains. 23

24. * Forensic Science II: Forensic Entomology, Chapter 12 © 2012 Cengage Learning. All Rights Reserved Postmortem Interval (Obj 12.4, 12.6) o Postmortem Interval—the time elapsed since a person died o When longer than 72 hours after death, entomology becomes important o Within 48 hours the female blowfly lays eggs in moist areas of the body

25. * Forensic Science II: Forensic Entomology, Chapter 12 © 2012 Cengage Learning. All Rights Reserved Collecting & Preserving Evidence (Obj 12.6, 12.7, 12.8) o Observe and record all pertinent factors Weather patterns (rainfall, wind, humidity, temperature, and exposure to light) during the PMI o Avoid disturbing insects within several feet of the body o Measure distance from body to insects o Collect samples of all insect stages on, near, and under the body

26. * Forensic Science II: Forensic Entomology, Chapter 12 © 2012 Cengage Learning. All Rights Reserved Collecting & Preserving Evidence (Obj 12.6, 12.7, 12.8) 1. Estimates on the types and number of insects 2. Key insect colonizations on and near the body 3. Exact positioning of the body using fixed points, if possible: placement of the arms and legs, the position of the head, and parts of the body in the shade and in the sunlight 4. Locations of eggs, larvae, and pupae on the body

27. * Forensic Science II: Forensic Entomology, Chapter 12 © 2012 Cengage Learning. All Rights Reserved Collecting & Preserving Evidence (Obj 12.6, 12.7, 12.8) 5. List of opportunistic feeders and other insects (such rove beetles, ants, wasps, and insect parasites) 6. Insect movements (within 20 feet of the body) 7. Natural and artificial influences that might affect the speed of decomposition, such as burning, covering, or burying the body

28. * Forensic Science II: Forensic Entomology, Chapter 12 © 2012 Cengage Learning. All Rights Reserved Collecting & Preserving Evidence

29. Stages of Decomposition o “Fresh”- signs of liver, algor, and rigor mortis Blowflies appear o “Bloat”- Abdominal cavity swells, strong odor Blowflies, cheese flies, Muscidae o “Active”- carcass deflates, gases released, liquefecation Blowfly larvae strongly present 29

30. Stages of Decomposition continued o “Advanced”- most flesh removed from carcass, odors fade Mass migration of blowfly larvae, arrival of skin beetles o “Dry”- almost no flesh remains Most flies and beetles leave, other insects arrive 30

31. Activity: Insect research o In groups of 4, research 5 insects in your area. o On an index card, write the following: Common and scientific name of insect Sketch of insect Diet of insect Habitat of insect Predators of insect, if any Forensic importance, if any o Good websites: www.bugguide.net www.enature.com http://www.njnaturenot es.com/Insects_Field_ Guide.html http://www.njnaturenot es.com/Insects_Field_ Guide.html http://www.insectidenti fication.org/insects-by- state.asp?thisState=N ew%20Jersey http://www.insectidenti fication.org/insects-by- state.asp?thisState=N ew%20Jersey 31 DUE TOMORROW!

32. DO-now o Hand in insect cards for your group (From Monday), clipped together. o Individually, read “Lori Ann Auker” on p. 352- 353. o In pairs with your neighbor (as in, don’t move your seat), answer “Think Critically” questions, p. 353 (ONE PER GROUP) 32

33. * Forensic Science II: Forensic Entomology, Chapter 12 © 2012 Cengage Learning. All Rights Reserved Factors Affecting Insect Activity o Natural environment and natural light o Natural environment and artificial light o Artificial environment and natural light o Artificial environment and artificial light

34. * Forensic Science II: Forensic Entomology, Chapter 12 © 2012 Cengage Learning. All Rights Reserved Temperature Readings o Ambient air 1 foot above the body 4 feet above the body o Ground Soil Surface 10 centimeters below 20 centimeters below o Center of a large infestation (if present)

35. * Forensic Science II: Forensic Entomology, Chapter 12 © 2012 Cengage Learning. All Rights Reserved More on Collecting and Preserving o Capturing live flies with nets o Pin flies to a board, or o Preserve with 80% alcohol and 20% water Adult flies Eggs Largest larvae

36. * Forensic Science II: Forensic Entomology, Chapter 12 © 2012 Cengage Learning. All Rights Reserved More on Collecting and Preserving o Label vials with case number collection time date geographic location the location of the insects on the victim the initials of the investigator

37. HW o For tomorrow: o “Sylvia Hunt” Case study, p. 353-354. Answer all questions o Chapter 12 Review, p. 356, questions 1-28 o For Friday: o Study for Test! Chapters 3 and 12 o Bring in a shoebox for your insect group. 37

38. o http://en.wikipedia.org/wiki/Forensic_entomol ogy#Factors 38

39. * Forensic Science II: Forensic Entomology, Chapter 12 © 2012 Cengage Learning. All Rights Reserved Chapter Summary o Forensic entomology is the analysis of insect evidence in legal cases. o Insects belong to the phylum Arthropoda, a group of organisms with jointed appendages, segmented bodies, and an exoskeleton. o The first documented use of insects to solve a homicide case was in China in AD 1235 o Since, scientists have added to the knowledge of insect behavior and life cycle.

40. * Forensic Science II: Forensic Entomology, Chapter 12 © 2012 Cengage Learning. All Rights Reserved Chapter Summary o Insect evidence is valuable in estimating the postmortem interval. o There are more than 700,000 known species of insects, but only a few have forensic value. o Female blowflies are usually the first insects to colonize a dead body. o The extent of decomposition—especially if it is unevenly distributed on the body—provides information for estimating the time (and perhaps manner) of death.

41. * Forensic Science II: Forensic Entomology, Chapter 12 © 2012 Cengage Learning. All Rights Reserved Chapter Summary o Entomologists record and analyze climate and weather information related to the insect samples. o Temperature, humidity, and other weather and climate elements affect the insects that colonize remains as well as the progression of the insect life cycle. o Processing and preserving insect evidence requires special care, techniques, and materials.