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Bruno De Fanti Anel EE230 University of California, Santa Cruz December 2011 LASER SCANNING CONFOCAL MICROENDOSCOPY
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1. Endoscopy 1.1 Principles 1.2 Inconvenients 2. Confocal Microscopy 2.1 Description and benefits 2.2 Basic principle 2.3 Scanning 2.4 Detector 3. Microendoscopy 3.1 Endoscopy + Confocal Microscopy 3.2 Fiber Bundle 3.3 Excitation 3.4 Fluorophores 3.5 Some images 4. Conclusion INDEX
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1.1 Principles Endoscopy: looking inside Minimally invasive procedure Wide variety of health issues detected 1 ENDOS COPY Non microscopy Requires biopsy
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1.2 Inconvenients Biopsy may be unnecessary Approach often fails for pre-cancerous problems and small cancerous growths. Example: colon cancer Solution: Microendoscopy EndoscopyBiopsyMicroendoscopy 1 ENDOS COPY
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2.1 Description Optical Imaging technique Increase optical resolution & contrast High quality images Benefits: Control depth of field Reduction of the out of focus glare Ability to collect serial optical sections from thick specimens 3D Images 2 CONFOCA L MICRO – SCOPY
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2.2 Basic principle 2 CONFOCA L MICRO – SCOPY
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2 CONFOCA L MICRO – SCOPY 2.3 Scanning
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2 CONFOCA L MICRO – SCOPY 2.4 Detector: Photomultiplier Tube Spot by spot scanning requiers: Sensitive Very Fast
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2 CONFOCA L MICRO – SCOPY 2.4 Detector (a)Confocal image along with the signal from the photomultiplier (b) Signal and Confocal image after applying a negative offset voltage to the photomultiplier. (c) Final signal and image after the gain has been adjusted to fill the entire intensity range.
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3.1 Endoscopy + Confocal Microscopy 3 MICRO ENDOS COPY
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3.2 Fiber bundle 140cm long 30.000 coherent fibers Single mode fiber Minimum bending radius 4cm 2.4μm core diameter NA 0.35 3.3μm core spacing Image area diameter 0.8mm Total diameter 0.95mm 3 MICRO ENDOS COPY
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3.3 Excitation: AOTF (Acousto-Optic Tunable Filter) Very fast & Multi source Intensity modulation Wavelenght discrimination Blanking flyback 3 MICRO ENDOS COPY
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3.4 Fluorophores Different molecules causes different emission 3 MICRO ENDOS COPY
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3.5 Some Images a) Pollen grain b) Mouse lung tissue c) Rat brain d) Fern root 3 MICRO ENDOS COPY
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Conclusion Minimal invasive High resolution No tissue extraction Earlier detection Scalable to actual endoscopes CONCLUSION
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THANKS FOR YOUR ATTENTION!!!
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http://www.laparoscopicconsultant.co.uk/endoscopy_services.html http://www.laparoscopicconsultant.co.uk/endoscopy_services.html http://www.auntminnie.com/index.asp?sec=ser&sub=def&pag=dis&ItemID=84424 http://www.auntminnie.com/index.asp?sec=ser&sub=def&pag=dis&ItemID=84424 http://www.nature.com/nm/journal/v17/n2/fig_tab/nm.2292_ft.html http://www.nature.com/nm/journal/v17/n2/fig_tab/nm.2292_ft.html http://www.ncbi.nlm.nih.gov/pubmed/16231800 http://www.ncbi.nlm.nih.gov/pubmed/16231800 http://www.tcrt.org/Optical-Systems-for-In-Vivo-Molecular-Imaging-of-Cancer-p-491-504- p11938.html http://www.tcrt.org/Optical-Systems-for-In-Vivo-Molecular-Imaging-of-Cancer-p-491-504- p11938.html http://www.patologia.es/volumen42/vol42-num3/42-3n02.htm http://www.patologia.es/volumen42/vol42-num3/42-3n02.htm http://elendoscopista.blogspot.com/2009/11/avances-en-endoscopia-endomicroscopia.html http://elendoscopista.blogspot.com/2009/11/avances-en-endoscopia-endomicroscopia.html http://www.floralvalefamilymedicine.com/Welcome/PatientEducationGuide/tabid/6635/ctl/Vi ew/mid/10466/Default.aspx?ContentPubID=348 http://www.floralvalefamilymedicine.com/Welcome/PatientEducationGuide/tabid/6635/ctl/Vi ew/mid/10466/Default.aspx?ContentPubID=348 http://mcnitt.com/my-first-endoscopy/ http://mcnitt.com/my-first-endoscopy/ http://www.sciencephoto.com/media/127158/view http://www.sciencephoto.com/media/127158/view http://sites.lafayette.edu/neur401-sp10/methodology/confocal-microscopy/ http://sites.lafayette.edu/neur401-sp10/methodology/confocal-microscopy/ http://withfriendship.com/user/sathvi/confocal-microscopy.php http://withfriendship.com/user/sathvi/confocal-microscopy.php http://elendoscopista.blogspot.com/2009/11/avances-en-endoscopia-endomicroscopia.html http://elendoscopista.blogspot.com/2009/11/avances-en-endoscopia-endomicroscopia.html http://www.floralvalefamilymedicine.com/Welcome/PatientEducationGuide/tabid/6635/ctl/Vi ew/mid/10466/Default.aspx?ContentPubID=348 http://www.floralvalefamilymedicine.com/Welcome/PatientEducationGuide/tabid/6635/ctl/Vi ew/mid/10466/Default.aspx?ContentPubID=348 http://mcnitt.com/my-first-endoscopy/ http://mcnitt.com/my-first-endoscopy/ http://www.sciencephoto.com/media/127158/view http://www.sciencephoto.com/media/127158/view http://sites.lafayette.edu/neur401-sp10/methodology/confocal-microscopy/ http://sites.lafayette.edu/neur401-sp10/methodology/confocal-microscopy/ http://withfriendship.com/user/sathvi/confocal-microscopy.php http://withfriendship.com/user/sathvi/confocal-microscopy.php REFERENCES
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http://elendoscopista.blogspot.com/2009/11/avances-en-endoscopia-endomicroscopia.html http://elendoscopista.blogspot.com/2009/11/avances-en-endoscopia-endomicroscopia.html http://www.floralvalefamilymedicine.com/Welcome/PatientEducationGuide/tabid/6635/ctl/Vi ew/mid/10466/Default.aspx?ContentPubID=348 http://www.floralvalefamilymedicine.com/Welcome/PatientEducationGuide/tabid/6635/ctl/Vi ew/mid/10466/Default.aspx?ContentPubID=348 http://mcnitt.com/my-first-endoscopy/ http://mcnitt.com/my-first-endoscopy/ http://www.sciencephoto.com/media/127158/view http://www.sciencephoto.com/media/127158/view http://sites.lafayette.edu/neur401-sp10/methodology/confocal-microscopy/ http://sites.lafayette.edu/neur401-sp10/methodology/confocal-microscopy/ http://withfriendship.com/user/sathvi/confocal-microscopy.php http://withfriendship.com/user/sathvi/confocal-microscopy.php http://en.wikipedia.org http://en.wikipedia.org REFERENCES
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