1. Events of The Cell Division Cycle Occur in a Particular Order

2. Entering Mitosis Before the Completion of S phase is Bad for Chromosomes and Therefore Bad for Cells

3. Cell Cycle Checkpoints Elledge, Science 274:1664 (1996)

4. Weinert and Hartwell, Science 246:629 (1989) Substrate-Product versus Intrinsic Control Mechanisms

5. Caffeine Abrogates the Replication Checkpoint Molecular Biology of the Cell © 1994 by Bruce Alberts et al

6. 0 hrs at res temp cdc9 (DNA ligase) 3 hrs at res temp Weinert and Hartwell, Science 246:629 (1989) rad9 is the First Cell Cycle Checkpoint Mutant cdc9 rad9

7. Weinert and Hartwell, Science 246:629 (1989) Cell Cycle Checkpoints Improve Cell Viability

8. Cell Cycle Checkpoints Why always negative regulation?

9. GO x 5GO x 6 Anaphase: Very different situations Not very different situations A Mechanism Involving Negative Regulation is More Robust

10. How do Cell Cycle Checkpoints Work?

11. Zhou and Elledge Nature 408, 433 - 439 (2000)

12. Elledge, Science 274:1664 (1996) Replication and Damage Checkpoints in Yeast

13. Inhibitory CDC2 Phosphorylation is a kinase is a phosphatase

14. G2 Arrest Induced by DNA Damage Signal Sensor Transducer Effector

15. Metaphase in a mammalian cell

16. The Metaphase to Anaphase Transition

17. The Spindle Assembly Checkpoint

19. The Function of RAD9 (Rad53=chk2) (Rad9 similar to BRCA1) (Mec1=ATR)