1. Mechanism of hormone action, signal transduction, 2 nd messengers and receptors MAHMOUD A. ALFAQIH BDS PhD Department of Physiology & Biochemistry Jordan University of Science & Technology, School of Medicine School of Medicine Reading material Devlin 6e Chapter 13

3. Overview of Hormones Mobile signals secreted by endocrine system for distant cell-cell communicationMobile signals secreted by endocrine system for distant cell-cell communication Occur as four major classes (and several minor ones)Occur as four major classes (and several minor ones) –Peptide hormones: tiny “proteins” from 3- 200 aa’s, water-soluble, active only after cleavage of the targeting “pre-sequence” and the inactive “prohormone” (insulin, glucagon) Continue………….

4. Overview of Hormones –Amino acid derivative hormones: derived from amino acids, water-soluble, some are neurotransmitters (epinephrine, T3, T4 ) –Eicosanoids: derived from arachidonate, minimally water-soluble, act locally (rather than through the bloodstream), mediate pain, inflammation –Steroid hormones: derived from cholesterol, fat-soluble, are carried to targets by carrier proteins

5. Common Characteristics of Hormones They occur and function at very low concentrations – 10 -6 to 10 -12 MThey occur and function at very low concentrations – 10 -6 to 10 -12 M Deliberately unstable – levels rise rapidly upon secretion, but fall fast when it stopsDeliberately unstable – levels rise rapidly upon secretion, but fall fast when it stops Biochemical response may be very rapid, by altering existing enzyme activities, or slower, where gene expression levels changeBiochemical response may be very rapid, by altering existing enzyme activities, or slower, where gene expression levels change Act through two receptor types: cell surface and nuclearAct through two receptor types: cell surface and nuclear Display remarkable specificityDisplay remarkable specificity Operate through the “cascade” principleOperate through the “cascade” principle

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7. Two Types of Hormone Receptor Hormones are present at very low concentration, thus receptor affinity must be very tightHormones are present at very low concentration, thus receptor affinity must be very tight Cell surface receptors bind hormone outside – a conformational change transduces the signal to the inside, resulting in a “second messenger” (e.g. cAMP)Cell surface receptors bind hormone outside – a conformational change transduces the signal to the inside, resulting in a “second messenger” (e.g. cAMP) Cytosolic/nuclear receptors bind and retain hormone, migrating to the nucleus as a hormone- receptor complex and then alter gene expressionCytosolic/nuclear receptors bind and retain hormone, migrating to the nucleus as a hormone- receptor complex and then alter gene expression

8. Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc. Figure 13.6 Structures of four second -messenger molecules.

9. An Overview of Hormone Classes

10. 10 1.Receptor tyrosine kinase 2.Receptor serine/threonine kinase

11. Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc. Figure 13.5 Basic characteristics of receptor subtypes as functionally distinct receptor proteins that bind a common extracellular signaling molecule.

12. ENZYME LINKED RECEPTORS Mechanism of hormone action, signal transduction, 2 nd messengers and receptors 04.12.8 12

13. Enzyme linked receptors; Receptor tyrosine kinases (RTKs)  Include receptor for insulin, Epidermal Growth factor (EGF), platelet derived growth factor (PDGF)  Most are single subunit receptors but some exist as multimeric complexes (Like insulin)  Amino end is extracellular (Ligand binding), carboxyl end is intracellular and contains catalytic domain

14. Enzyme linked receptors; Receptor tyrosine kinases (RTKs)  Ligand binding induces receptor dimerization and activates catalytic domain  Catalytic domain auto-phosphorylates receptor (receptor also functions as an effector protein)  Phosphorylated tyrosine residues become anchoring point for adaptor proteins  Adaptor proteins have special protein-protein interaction domains called (SH2 for SRC Homology 2)  RTKs allow accumulation of many types of tyrosine phosphorylated proteins (secondary effector proteins)

15. Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc. Figure 13.14 Conformational and functional changes in a receptor tyrosine kinase during activation by growth factor binding. Redrawn based on figure from Alberts, B., et al., Molecular Biology of the Cell, 4th ed. New York: Garland Science, 2002. Insulin, EGF, PDGF

16. Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc. Figure 13.15 Mutated forms of receptor tyrosine kinases as the products of cancer-causing oncogenes. Mutations in RTKs can lead to cancer

17. GTP binding regulatory proteins  They could be functionally coupled to “G protein coupled receptor” (Exist as trimers)  They could exist as functionally distinct monomeric proteins  They have GTPase activity which allows them to function as “molecular switches”  Exchange of GDP with GTP activates the proteins 17

18. GTP binding regulatory proteins 18

19. Ras GTPase and MAP Kinases  Ras is a small monomeric GTP binding regulatory protein  About 30% of all tumors have mutations in Ras GTPase  Adaptor proteins can directly bind to phosphorylated RTKs and recruit Ras activating proteins  Ras activating proteins enhance GDP exchange with GTP  Ras protein can then activate downstream signaling pathways involved in proliferation (MAP kinase cascade)

20. Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc. Figure 13.16 Role of the ras GTPase during intracellular signal transduction by an activated receptor tyrosine kinase.

21. Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc. Figure 13.17 Role of the MAP kinase cascade during intracellular signal transduction by an activated receptor tyrosine kinase. Redrawn based on figure from Alberts, B., et al., Essential Cell Biology, 2 nd ed. New York: Garland Science, 2004. Ras GTPase and MAPK

22. Signaling by protein phosphorylation 22

23. Receptor Serine/Threonine Kinase  Receptors for transforming growth factor B (TGFB)  Important role in cell signaling during tissue differentiation and fetal development  Mutations in TGFB mediated signaling pathway leads to cancer 23

24. Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc. Figure 13.18 Redrawn based on figure from Alberts, B., et al., Essential Cell Biology, 2 nd ed. New York: Garland Science, 2004. Receptor Serine/Threonine kinases

25. G PROTEIN COUPLED RECEPTORS Mechanism of hormone action, signal transduction, 2 nd messengers and receptors 25

26. G protein coupled receptors (GPCRs)  GPCRs bind to a diverse range of ligands (proteins, peptides, amino acid derivatives, lipids, nucleotides)  Play an important role in endocrine, paracrine, autocrine signaling in all tissues and cell types  Sensory proteins just like rhodopsin are GPCRs  GPCRs, their ligands and their downstream pathways are targets for many of the currently used drugs  GPCRs have a great degree of structural but not sequence similarity 26

27. GPCRs 04.12.8 27

28. Heterotrimeric G proteins  GPCRs are functionally coupled to trimeric complexes consisting of α, β and γ.  α subunit is the largest subunit, hydrophilic and contains GTPase activity  Twenty subtypes of α subunit are grouped into subfamilies based on specific downstream action  α s Subunit stimulates adenylate cyclase, while α i inhibits it 28

29. Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc. Figure 13.21 Major subclasses of heterotrimeric G proteins that are activated by G by the seven- transmembrane domain G-protein-couple receptors. Heterotrimeric G proteins

30. Heterotrimeric G protein cycle 30

31. Termination of signaling by GPCRs 31

32. Cyclic AMP based signal transduction  The use of cAMP as a second messenger is largely limited to GPCRs  Cells regulate both the synthesis and the degradation of cAMP  cAMP synthesis is catalyzed by adenylate cyclase effector enzyme  An integral membrane protein  At least six genes that encode different subtypes have been identified

33. Cyclic AMP based signal transduction  All subtypes are activated by α s Subunit of G proteins and also inhibited by α i subunit  Breakdown of cAMP is catalyzed by a variety of phosphodiesterases that hydrolyze ester bond and lead to the production of AMP  Activity of phosphodiesterase is hormonally regulated and is affected by drugs  Caffeine inhibits activity of phosphodiesterases leading to accumulation of cAMP

34. Breakdown of cAMP 34

35. Adenylate cyclase activity Activated adenylyl cyclase makes cAMP, the “2 nd messenger”,Activated adenylyl cyclase makes cAMP, the “2 nd messenger”, Which activates “PKA” which phosphorylates other proteins,Which activates “PKA” which phosphorylates other proteins, Such as phosphorylase kinase, which in turn Phosphorylates glycogen phosphorylase This mobilize glucose from liver glycogen!This mobilize glucose from liver glycogen! Protein kinase A

36. Intracellular signaling mechanism of cAMP 36

37. Turn off of the signal: 1. G  hydrolyzes GTP to GDP + P i. (GTPase). The presence of GDP on G  causes it to rebind to the inhibitory  complex. Adenylate Cyclase is no longer activated. 2. Phosphodiesterase catalyzes hydrolysis of cAMP  AMP. cAMP  AMP.

38. Turn off of the signal (cont.): 3. Receptor desensitization occurs. This process varies with the hormone.  Some receptors are phosphorylated via specific receptor kinases.  The phosphorylated receptor may then bind to a protein arrestin, that promotes removal of the receptor from the membrane by clathrin-mediated endocytosis. 4. Protein Phosphatase catalyzes removal by hydrolysis of phosphates that were attached to proteins via Protein Kinase A.

39. Signal amplification is an important feature of signal cascades:  One hormone molecule can lead to formation of many cAMP molecules.  Each catalytic subunit of Protein Kinase A catalyzes phosphorylation of many proteins during the life-time of the cAMP.

40. Activation of PKA heterotetramer (inactive) dimer active monomers

41. Cyclic GMP based signal transduction  Levels of cGMP are regulated by a balance between guanylate cyclase and cGMP phosphodiesterase  Guanylate cyclase could be either membrane bound or a free cytosolic protein (soluble)  Membrane bound guanylate cyclase could be activated by ANF (atrial natriuretic factor)  Soluble Guanylate cyclase is activated by NO (nitric oxide) 41

42. Cyclic GMP based signal transduction  Activation of guanylate cyclase leads to relaxation of vascular smooth cells through activation of cGMP sensitive protein kinases  Mechanism: 1.Attenuation of calcium release from sarcoplasmic reticulum 2.Activation of myosin light chain phosphatases 3.Activation of potassium channels leading to hyperpolarization of myocyte membrane 42

43. Signaling mechanism of cGMP 43