1. XTRTACT – C 6939 All new natural Anticoccidiostat

2. What is XTRACT-C? XTRACT-C is… Complex Additive composed of Curcumin(Tumeric), Cavacrol, Capsaicin, Cinnamaldehyde. Four components have each stimulating digestion enzyme activities, immune responses, sterilization effects and various biological effects.

3. Quality control of XTRACT Pancosma is aware of the feed security stakes involved and has confined its research in substances at a world-wide level in human and animal nutrition. Moreover, all the active principles selected are classified by FDA (Food&Drug Administration, USA) as GRAS (Generally Recognised As Safe). Pancosma has anticipated in his research the principle of precaution. Within this principle of global precaution. AXISS also includes the standardization on active substances and the quality control. Nevertheless, wishing to reassuring always more the consumer, Pancosma has undertaken a huge effort in the quantitative and qualitative traceability of active substances in the animal tissues NameCAS No% in the composition Tumeric oleoresin8024-37-18 Cinnamaldehyde104-55-21.2 Carvacrol499-75-22 Capsicum oleoresin8023-77-60.8

4. MODE OF ACTION

5. The effect of substances What is CAPSAICIN? Stimulating enzyme activities EFFECT OF CAPSAICIN IS TO STIMULATE PANCREATIC AND SMALL INTESTINE ENZYMES EFFECT OF CAPSAICIN IS TO STIMULATE PANCREATIC AND SMALL INTESTINE ENZYMESCAPSAICIN TRYPSIN AMYLASE LIPASE PROTEIN DIGESTION STARCH DIGESTION FAT DIGESTION

6. * Effect of Capsaicin in diets on the activity of small intestine digestive enzymes * Effect of Capsaicin in diets on the activity of pancreatic digestive enzymes LIPASE AMYLASE TRYPSIN CHYMOTRYPSIN CONTROL 30.5 47.0 2.59 2.89 CAPSAICIN 41.4* 80.9* 5.70* 3.75* Enzyme activity expressed in IU/ml * Significantly higher than control (p<0.05). LIPASE AMYLASE SUCRASE LACTASE MALTASE CONTROL 23.1 0.021 71.5 31.0 534.4 CAPSAICIN 67.2* 0.025* 98.6* 33.9 670.5* Source : Platel and Srinivasan, Nahrung (2000) / Platel and Srinivasan, Int. J. Aliment Sci. (1996) The effect of substances

7. CINNAMALDEHYDE : Anti-Oxidant ACTIVITY OF MUCOSAL ANTIOXIDANT ENZYMES SUPEROXIDE DISMUTASE (IU/mg protein) GLUTATHIONE S-TRANSFERASE (nmoles NADPH oxidised /min/mg protein) CATALASE (mg x 10 -3 mg protein) CONTROL 9.43.664.0 CINNAMALDEHYDE 15.8 *4.5 *88.8 P 0.05 NS SOD expressed in IU/mg protein; GST expressed in nmoles NADPH oxidized/min/mg protein; glutathione expressed in mg x 10 -3 mg protein * Significantly higher from control (p<0.05). Source : JN Dhuley, Indian Journal of Experimental Biology, 37:23-42 (1999) The effect of substances

8. CARVACROL IN VITRO LEVELS PHYSIOLOGICAL LEVELS Source : Kivanç et al., Int. J. Food Microbiol (1991) POSSIBILITY OF RESISTANCE CARVACROL: Stimulates Gram+, Lactic acid bacteria (AT LOW PHYSIOLOGICAL LEVELS) Kills Gram- but also Grma+ organism including those in most probiotic preparations Stimulates Gram+ such as lactobacillus and pediococcus found in many probiotic preparations The effect of substances

9. HO CH 3 O CHCHCOCH 2 COCHCH OCH 3 OH Tumeric/Curcumin : Biological effect Tumeric/Curcumin Enhance Wound healing Anti-inflammatory Anti-Oxidant activity Anti-mutagenic Anti-metastatic Anti-angiogenic Immunomodulatory Prevents IRI/ Neuroprotection Anti-viral Anti-fungal Anti-bacteria The effect of substances

10.  Phenols such as Carvacrol, Cinemaldehyde have been proven to contain anticoccidial properties  Beside their phenolic mode of action against pathogenic bacteria such as Clostridium perfringens they exert a different mode of action against intracellular protozoan- parasites such as Eimeria spp. - therefore to prevent and treat coccidiosis and necrotic enteritis in poultry and swine. The effect of substances

11.  Phenols act on the gut lining (which is regularly shed in a healthy intestinal mucosae) by increasing the rate of nterocyte turnover by about 2x the normal rate (De asis 2002, Tsinas 1999, Ultee et al. 1999, Weber 1996).  The phenolic compounds disrupt the lifecycle of coccidian parasites by speeding up the rate of shedding of the gut lining – therefore intracellular coccidial sporozoites (which multiply in the epithelial cells of the intestine) are shed before they have the opportunity to penetrate deeper into the gut wall or to form new oocysts (Tsinas et al. 1998, Chapman 1988). The effect of substances

12. Trial Results Anticoccidial effect of Xtract-C By CP group in Thailands

13. Consumptions and Temperatures Experimental context: Experimental context: 840 broilers SASSO 451 N nacked neck in “Label” conditions - 6 pens of 14 0 individus (70 males et 70 femelles) inoculated at 14 days per os with a dose of: 100000 oocystes of E. acervulina, 10000 oocystes of E. tenella and 1000 oocystes of’E. maxima. Source : ENSAT, France 2003. Trial n° 03 COC 018

14. Live weight between 4 and 8 weeks in comparison to monensin (CP group trial) Experimental context: Experimental context: 840 broilers SASSO 451 N nacked neck in “Label” conditions - 6 pens of 14 0 individus (70 males et 70 femelles) inoculated at 14 days per os with a dose of: 100000 oocystes of E. acervulina, 10000 oocystes of E. tenella and 1000 oocystes of’E. maxima. Source : ENSAT, France 2003. Trial n° 03 COC 018 6986 R&D at 500 ppm: weights near from monensin group 27days 41days 55days 83days Blank

15. Excretions ookystales fecales Experimental context: Experimental context: 840 broilers SASSO 451 N nacked neck in “Label” conditions - 6 pens of 14 0 individus (70 males et 70 femelles) inoculated at 14 days per os with a dose of: 100000 oocystes of E. acervulina, 10000 oocystes of E. tenella and 1000 oocystes of’E. maxima. Source : ENSAT, France 2003. Faecal oocystes excreted at 21, 27, 41 et 70 days of age No statistics availableTrial n° 03 COC 018 days Blank Decrease oocyst excretion

16. Trial Results Pathogenic bactericidial effect of Xtract-C

17. Essential Oils and Clostridium perfringens - in feces 14 21 30 day Figure 1.Relative frequencies of Clostridium perfringens concentrations per gram of sample in the feces of the control group © and groups with essential oils blend A(thymol, eugenol, Curcumin, and piperin) and B(thymol, carvacrol, eugenol, curcumin, and piperin) on d 14, 21, and 30. 2004 Poultry Science Association, Inc. Corresponding author : pu.mitsch@utanet.at

18. Essential Oils and Clostridium perfringens - in jejunum Figure 1.Relative frequencies of Clostridium perfringens concentrations per gram of sample in the jejunum of the control group © and groups with essential oils blend A(thymol, eugenol, Curcumin, and piperin) and B(thymol, carvacrol, eugenol, curcumin, and piperin) on d 14, 21, and 30. 2004 Poultry Science Association, Inc. Corresponding author : pu.mitsch@utanet.at 14 21 30 day

19. Dosage of XTRACT-C SpeciesDosage Piglets300 ~ 350g/ton Grower pigs150 ~ 200g/ton Calves250 ~ 300g/ton Full-grown calf150g/ton