1. Frankie Jernigan/ Kathy Roeske FD36/Operations Development Group 256-544-2487/256-544-6450 Frankie.jernigan@nasa.govFrankie.jernigan@nasa.gov /Kathy.Roeske@msfc.nasa.gov Protein Crystal Growth Monitoring by Digital Holographic Microscope 2 and3 (PromISS 2 and 3)

2. 2 LESSON OBJECTIVES Explain the PromISS 3 science objectives Describe the PromISS 2 and 3 hardware used on board the ISS Summarize the overall operations of PromISS 3 Discuss PromISS 2 on-orbit procedural flow

3. 3 LESSON OUTLINE Payload Description Payload Hardware Microgravity Science Glovebox (MSG) Interfaces Payload Operations Safety Manual Procedure Overview Summary

4. PAYLOAD DESCRIPTION

5. 5 PAYLOAD DESCRIPTION (cont.) Experiment Timeframe Launch on Progress/Soyuz Operations in Microgravity Science Glovebox, Increment 8

6. PAYLOAD HARDWARE

7. 7 Hardware Overview The PromISS 2 hardware consists of: –Experiment Box –PromISS 2 Kit 1 Electronics Box –PromISS 2 Kit 2 Software Upload Cable Sample Wheel Locking System Clamp –Video Camera (MSG Provided)

8. 8 PAYLOAD HARDWARE Hardware Overview The PromISS 3 hardware consists of: –PromISS 3 Kit 1 Return Container 12 Video Tapes –PromISS 3 Kit 2 Sample Container Six Sample Cells

9. 9 PAYLOAD HARDWARE (cont.) FRONT VIEW REAR VIEW EXPERIMENT BOX

10. 10 PAYLOAD HARDWARE (cont.) EXPERIMENT BOX Internal Components PromISS 2 is an interferometric microscope that is composed of 5 optical systems that are contained in the Experiment Box and not accessible by the crew.

11. 11 EXPERIMENT BOX (cont.) External Components (accessible by crew) - Two momentary switches -Three LEDs - Camera mount - One POWER/DATA connector - Cover Lid PAYLOAD HARDWARE (cont.)

12. 12 EXPERIMENT BOX (cont.) The three LEDs illuminate green -WAIT TAPE will illuminate green when WAIT TAPE momentary switch is toggled to the up position -RUNNING will illuminate green when the MSG EXP POWER is enabled -END RUN will illuminate green when the END RUN momentary switch is toggled to the up position PAYLOAD HARDWARE (cont.)

13. 13 EXPERIMENT BOX (cont.) The two momentary switches -WAIT TAPE will halt the sample wheel rotation and stop recording for tape exchanges, operator will toggle once to the up position prior to tape removal (LED illuminates green), and then up after tape exchange is complete (LED extinguishes, RUNNING LED illuminates) -END RUN will initiate the SAMPLE WHEEL rotation to the zero position. When toggled to the up position, the END RUN LED will illuminate and remain green until the SAMPLE WHEEL has reached the zero position. Depending on the position of the SAMPLE WHEEL when the switch is toggled, the time to zero position may take up to 1 hour. PAYLOAD HARDWARE (cont.)

14. 14 EXPERIMENT BOX (cont.) POWER/DATA Connector -POWER/DATA (J308) will provide the power and data interface between MSG and the EXPERIMENT BOX The Cover Lid -Provides for a level of containment when properly sealed The Camera Mount -Provides the hardware interface for the MSG Video Camera. The camera must be firmly seated within the mount or the micro switches that are in the bottom of the mount will not activate. When securing the camera the crew needs to listen for the click of the micro switches. During activation there is also a check with the POIC for data confirmation that the micro switches are enabled. PAYLOAD HARDWARE (cont.)

15. 15 PAYLOAD HARDWARE (cont.) PromISS 2 Kit 1 and 2 PromISS 2 Kit 1PromISS 2 Kit 2

16. 16 PAYLOAD HARDWARE (cont.) PromISS 2 Kit 1 and Kit 2 PromISS 2 Kit 1 contents: PromISS 2 Electronics box with pigtailed harness PromISS 2 Kit 2 contents: PromISS 2 Sample Wheel PromISS 2 Locking System PromISS 2 Clamp PromISS 2 Software Upload Cable

17. 17 PAYLOAD HARDWARE (cont.) ELECTRONICS BOX

18. 18 PAYLOAD HARDWARE (cont.) ELECTRONICS BOX The ELECTRONICS BOX is fitted with 3 M6 Captive Screws that are identical to the ones in the PROMISS 2 EXPERIMENT BOX. There are 3 pigtailed harnesses protruding from the ELECTRONICS BOX: POWER CABLE ->MSG (P325) DATA CABLE ->MSG (P311) POWER AND DATA CABLE ->PROMISS 2 EXPERIMENT BOX (P308). The ELECTRONICS BOX is also fitted with one permanent toggle switch (either OPERATION or UPLOAD mode) that enables the Crew to perform a S/W upload, or operate normally the PROMISS 2 EXPERIMENT BOX. The toggle switch is protected by switch guards.

19. 19 PAYLOAD HARDWARE (cont.) SOFTWARE UPLOAD cable Software Upload P311

20. 20 PAYLOAD HARDWARE (cont.) SOFTWARE UPLOAD cable This cable will only be used in the event that a software upload to the PromISS 2 ELECTRONICS Box is required. The following steps represent the operations required to perform the software upload. –The ground will perform a software upload from the ground to the MLC via the Payload MDM. –The crew will need to connect the SOFTWARE UPLOAD cable to the MLC and the PromISS 2 ELECTRONICS BOX. Place the OPERATIONS/UPLOAD switch in the UPLOAD position. –The ground will send a ground command to transfer the new software load from the MLC to the PromISS 2 ELECTONICS BOX. –The crew will disconnect the SOFTWARE UPLOAD cable, reconnect the MLC and place the OPERATIONS/UPLOAD switch in the OPERATIONS position. The cable was labeled incorrectly and a hand written label was placed on the cable at Baikonur.

21. 21 PAYLOAD HARDWARE (cont.) PromISS 3 Kit 2

22. 22 PAYLOAD HARDWARE (cont.) PromISS 3 Balltip L-Wrench

23. 23 PAYLOAD HARDWARE (cont.) PromISS 3 Balltip L-Wrench The PromISS 3 Balltip L-Wrench is a PromISS specific tool that is used for the fasteners on the Sample Container and Return Container. It is stowed in the upper foam of the PromISS 3 Kit 2.

24. 24 PAYLOAD HARDWARE (cont.) SAMPLE CONTAINER (PromISS 3)

25. 25 PAYLOAD HARDWARE (cont.) SAMPLE CONTAINER (PromISS 3) The SAMPLE CONTAINER consists of two sealed cavities that are fastened together using 12 stainless steel M6 captive screws. Each cavity contains Phase Change Material (PCM) that will stay frozen at 22° C when faced with an external temperature of 30° C. This will allow the sample cells to be kept at a more constant temperature. The six sample cells are placed in the SAMPLE CONTAINER insert. The SAMPLE CONTAINER provides two levels of containment with two separate Viton o-rings. Once opened the two main cavities remain fastened together with two Kevlar cords that link them. The SAMPLE CONTAINER is also fitted with a pressure valve, in order to allow its easy opening into MSG WV. All the captive screws to be handled by the Crew are of Hex6 type.

26. 26 PAYLOAD HARDWARE (cont.) SAMPLE CONTAINER insert (PromISS 3)

27. 27 PAYLOAD HARDWARE (cont.) SAMPLE CONTAINER insert (PromISS 3) The SAMPLE CONTAINER insert holds the sample cells within the SAMPLE CONTAINER. The insert is removed from the SAMPLE CONTAINER and will be used in the RETURN CONTAINER to hold the samples for storage in the Russian Incubator Aquarius and return on Soyuz. The SAMPLE CONTAINER insert is not labeled.

28. 28 PAYLOAD HARDWARE (cont.) SAMPLE CELLS (PromISS 3)

29. 29 PAYLOAD HARDWARE (cont.) SAMPLE CELLS (PromISS 3) The SAMPLE CELLS are contained in the SAMPLE CONTAINER until they are installed on the SAMPLE WHEEL for integration into the EXPERIMENT BOX. There are six samples with six capillaries per sample. Some of the samples are not including capillaries, but their external housing is always identical. There is approximately 8 seconds between capillaries and 10 minutes between experimental cell rotations. Peak power is expected between sample cell rotation.

30. 30 PAYLOAD HARDWARE (cont.) SAMPLE WHEEL

31. 31 PAYLOAD HARDWARE (cont.) SAMPLE WHEEL The SAMPLE WHEEL will contain the six sample cells. The SAMPLE CELLS will slide into place until the ball lock engages. The Crew has to install the 6 sample cells onto the SAMPLE WHEEL. The Crew uses a Locking System that temporarily secures the SAMPLE WHEEL onto the MSG rear wall. The Crew will insert the SAMPLE CELLS one by one onto the SAMPLE WHEEL. Each SAMPLE CELL is numbered to correspond to the numbers on the SAMPLE WHEEL. One complete SAMPLE WHEEL rotation takes approximately 1 hour when PROMISS 2 EXPERIMENT is operating in nominal mode.

32. 32 PAYLOAD HARDWARE (cont.) LOCKING SYSTEM

33. 33 PAYLOAD HARDWARE (cont.) LOCKING SYSTEM The Locking System is used to temporarily secure the SAMPLE WHEEL to the MSG rear wall so that the SAMPLE CELLS can be installed onto the SAMPLE WHEEL.

34. 34 The PromISS CLAMP is a mechanical interface between the PromISS EXPERIMENT BOX and the MSG Airlock Lid. It is one of three fasteners that secures the PromISS to the Airlock Lid, the remaining two are fixed to the EXPERIMENT BOX. NOTE: PromISS is installed to the MSG Airlock Lid. All cooling is via WV air circulation not the MSG cold plate. The PromISS CLAMP attaches to the rear of the EXPERIMENT BOX PAYLOAD HARDWARE (cont.) PromISS CLAMP

35. 35 PAYLOAD HARDWARE (cont.) PromISS 3 TAPES PromISS 3 Kit 1

36. 36 PAYLOAD HARDWARE (cont.) PromISS 3 TAPES There are twelve digital tapes that will be used to record the PromISS 3 Video. Tape exchanges 1-5 are every 36 hours. Tape exchanges 6- 10 are every 72 hours. Tape exchange 11 is done after 72 hours. Tape 1 is installed during PromISS 2 SETUP, Tape 12 is removed during PromISS 2 STOW.

37. 37 PAYLOAD HARDWARE (cont.) PromISS 3 Return Container Pressure Valve

38. 38 PAYLOAD HARDWARE (cont.) PromISS 3 Return Container

39. 39 PAYLOAD HARDWARE (cont.) PromISS 3 RETURN CONTAINER The PromISS 3 RETURN CONTAINER will be used for sample return on 7S. It has 10 fasteners that secure the lid and a pressure valve. The pressure valve did not get labeled prior to flight, but is operated exactly the same as the SAMPLE CONTAINER pressure valve. The previous slide shows the RETURN CONTIANER flight unit and how it should have been labeled. The PromISS 3 RETURN CONTAINER will be stored in the MSG WV during the PromISS 3 operations. The RETURN CONTAINER has sugar cubes in a sealed cavity in the bottom. These sugar cubes are used to record temperature and acceleration data during the PromISS 3 operations. The insert from the SAMPLE CONTAINER will be placed in the RETURN CONTAINER during stow. The samples will be stored in the RETURN CONTAINER in the Russian incubator Aquarius until they are brought back on 7S.

40. MSG INTERFACES

41. 41 MSG INTERFACES RESOURCE/REQUIREMENT PromISS 2 EXPERIMENT POWER OUTLETS EXP. OUTLET 3 +120V (J302) (8.3A/996W) NA ICP (J304)NA EXP. OUTLET 1 (J303)+5V (4A/20W) (2A/24W)  12V (8.5A/238W)+28V EXP. OUTLET 2 (J325) +5V X  12V X +28V X

42. 42 MSG INTERFACES (cont.) FUNCTIONS AIR CIRC. FANSPEED MODE 0 --- 4, 7 (MAX) 4 (Default) MODE (NOR/MAN)NOR WV ILLUM (80% Nominal) 40% --- 100% (MAX) 80% (setup) off during Ops AL ILLUM (Yes/No)No RESOURCE/REQUIREMENT PromISS 2

43. 43 MSG INTERFACES (cont.) VIDEO CAMERA 1 (J201) (Color/BW) CAMERA 2 (J202) (Color/BW)X (Color) CAMERA 3 (J203) (Color/BW) CAMERA 4 (J204) (Color/BW) RECORDER 1 (Digital) RECORDER 2 (Digital)X (12) RECORDER 3 (8mm) RECORDER 4 (8mm) Monitor (1, 2)1* Headset/Microphone Digital Still Imagery (not MSG)X RESOURCE/REQUIREMENT PromISS 2 *The second MSG Monitor will be used for operating the Video System GUI.

44. 44 MSG INTERFACES (cont.) COMMAND & DATA HANDLING EXP. DATA 1 (J301) EXP. DATA 2 (J319) RS422 (J305) RS422 (J311)X RS422 CMP (J312) ETHERNET (J320) Default Lan-2 J403 Data Feed Through (Parallel) J404 Data Feed Through (Serial) RESOURCE/REQUIREMENT PromISS 2

45. 45 RESOURCE/REQUIREMENT PromISS 2 MLC External (Ext)/Internal (Int)Ext 1553B-A (J318)X 1553B-B (J314)X J46 LAN-1 (UIP Ethernet MLC Default) X J47 LAN-2 (UIP Ethernet) Microdrive Data Storage (Yes/No) No OTHER SAMS (Non MSG)X MAMSX PERSX MSG INTERFACES (cont.)

46. 46 PAYLOAD OPERATIONS/PROCEDURE OVERVIEW

47. 47 PAYLOAD OPERATIONS/ PROCEDURE OVERVIEW PromISS is automated and has two ground commands, one for data table uplink (the data table update is for changing parameters for the piezo and ground glass motor settings) and one for software load (this will be used in the off nominal situation that the software is corrupted. There is one Sample Wheel that processes continually once activated (current timeline is 30 days) Required crew/operation time -PromISS 2 SETUP for 75 minutes (PromISS Hardware, and Tape 1 install) -PromISS 2 ACT for 10 minutes (activates PromISS via CMP PWR OUT 2) -PromISS 3 PROCESSING for 30 days (unattended) -PromISS 2 VIDEO OPS for 10 minutes (11 exchanges, TAPE 1 is installed at SETUP, TAPE 12 is removed at STOW) -PromISS 2 STOW for 55 minutes will power down the payload and remove all hardware from MSG, stow for return

48. 48 PAYLOAD OPERATIONS/ PROCEDURE OVERVIEW (cont.) PromISS 2 SAFETY OVERVIEW MSG-G1 (Electrical Shock) MSG-G2 (Ignition Source in the Working Volume) MSG-G4 (Pinching, Scissoring of Crew Fingers) PromISS-01 (Release of Sample Material) PromISS-03 (Electrical Shock) PromISS-04 (Crew Contact with Surfaces at Temperatures Above Touch Temperature Limits)

49. 49 Hazard Report Number Hazard Control Number Hazard Control SummaryProcedure Filename(s)/ Step(s) MSG-G1 4 Electrical Shock Operational procedures will direct the crew to interrupt the power line prior to mating/demating of power connectors. MGUEMSGPRMS2N001, Steps 2.13, 3.5 MGUEMSGPRMS2N003, Steps 3.6, 3.7 MSG-G2 6.2 Ignition Source in the Working Volume Crew procedures will direct operator to interrupt power line via CMP prior to mating/demating of connectors. Power mode indication (ON/OFF) is provided by the CMP via LED. MGUEMSGPRMS2N001, Steps 2.13, 3.5 MGUEMSGPRMS2N003, Steps 3.6, 3.7 PromISS 2 SAFETY OVERVIEW PAYLOAD OPERATIONS/ PROCEDURE OVERVIEW (cont.)

50. 50 Hazard Report Number Hazard Control Number Hazard Control SummaryProcedure Filename(s)/ Step(s) MSG-G4 2.2 Pinching, Scissoring of Crew Fingers Extraction/insertion of the Glovebox is a two-hand operation. One hand is operating the knob, while the other hand should be holding a handle attached to the seat tracks to apply the force needed for operating the sliding mechanism. The handle is not part of MSG design. However, it is recommended, and requested by the crew during the MSG Crew Review to make use of ISS handles that can be attached to the seat tracks where convenient for the operator. Operations of the sliding mechanism will be described in a crew procedure including potential pinching and scissoring hazards that might occur during operations. MGUEMSGPRMS2N001, Steps 1.11, 6.4 MGUEMSPRMS2N003, Steps 1.1, 6.13 MGUEMSPRMS2C001, Step 2. PAYLOAD OPERATIONS/ PROCEDURE OVERVIEW (cont.) PromISS 2 SAFETY OVERVIEW

51. 51 Hazard Report Number Hazard Control Number Hazard Control SummaryProcedure Filename(s)/ Step(s) MSG-GB1 4.3 4.5 8.1 11.1 Release of Hazardous Material Inspection of gloves prior to start of experiment and replacement (per crew procedures) Provision of closing cap to be inserted on glove port in case of glove rupture. Cleaning equipment to be provided inside Glovebox (unique for each experiment). Experiment responsibility, supported by MSG. Provision for maintenance procedures (included in the crew procedure). MGUEMSGPRMS2N002, Step 1.1 MGUEMSGPRMS2N001, Step 6.1 MGUEMSGPRMS2N001, Step 1.16 MGUEMSGPRMS2N001, Steps 4.4, 4.22, 4.23,4.27,4.44 MGUEMSPRMS2N003, Step 4.16 PAYLOAD OPERATIONS/ PROCEDURE OVERVIEW (cont.) PromISS 2 SAFETY OVERVIEW

52. 52 Hazard Report Number Hazard Control Number Hazard Control SummaryProcedure Filename(s)/ Step(s) PROMISS-01 1.2 Release of Sample Material Crew procedures require leak tight closing (hand tight) and visual inspection of the PCM Samples Container through MSG window prior to opening the MSG WV. MGUEMSPRS2N001, Steps 4.4, 4.22,4.44 MGUEMSPRS2N003, Steps 4.16 MGUEMSPRMSC001, Steps 12 PromISS-03 2.1 2.2 Electrical Shock Crew procedures require removal of power is cut via MSG switch and waiting approximately 10 msec for capacitor discharge before any crew activity on PromISS. PromISS shall comply with Letter MA2-99-170, Category 2 requirements. The high voltage connector is only accessible during installation of the PromISS Experiment Box and PromISS Electronics Box. MGUEMSGPRS2N001, Steps 2.13, 3.5 MGUEMSGPRS2N003, Steps 3.6, 3.7 MGUEMSGPRS2N001 Step 2.20 PAYLOAD OPERATIONS/ PROCEDURE OVERVIEW (cont.) PromISS 2 SAFETY OVERVIEW

53. 53 Hazard Report Number Hazard Control Number Hazard Control SummaryProcedure Filename(s)/ Step(s) PromISS-04 1.3 Crew contact with surfaces at temperatures above touch temperature limits Crew procedure requires to verify function of water cooling loop and air temperature of MSG WV on MSG I&cc before touch Electronic box. Crew procedure to include the introduction of a waiting time sufficient for all accessible surfaces of the electronic box to reach a temperature below the touch temperature limit (10 min waiting time, see thermal analysis report TAXI-RP-289- VE) in case of non functioning of water cooling loop MGUEMSGPRMS2N002, Step 1.3 MGUEMSGPRMS2N003, Step 3.2 MGUEMSGPRMS2C001, Step 4 MGUEMSGPRMS2N006, Steps 1.5 PAYLOAD OPERATIONS/ PROCEDURE OVERVIEW (cont.) PromISS 2 SAFETY OVERVIEW

54. 54 PAYLOAD OPERATIONS/ PROCEDURE OVERVIEW (cont.) MSG VIDEO SETUP (MGUEMSGN008) START MLC EXTERNAL SETUP (MGUEMSGN011) MSG ACTIVATION (MGUEMSGN002) 10 min PromISS 2 VIDEO OPERATIONS (Exchange TAPES 2 --- 11) (MGUEMSGPRS2N004) PromISS 2 SETUP (Install tape 1 into MSG VCR2) (MGUEMSGPRS2N001) PromISS 2 ACTIVATION (MGUEMSGPRS2N002) PromISS 3 Processing (UNATTENDED OPERATIONS) 75 min 10 min 30 days 1 112 MSG STANDBY/ POWER DOWN (MGUEMSGN013) 55 min 1 PromISS 2 STOW (MGUEMSGPRS2N003) 3 1. Two levels of containment are maintained during transfer and setup into MSG. During transfer the cells and the SAMPLE CONTAINER/RETURN CONTAINER serve as levels of containment. Once removed from the SAMPLE CONTAINER/RETURN CONTAINER the MSG WV is sealed and air circulation is on (~3.5mbar), so two levels of containment are still maintained 2. During Stow two levels of containment are maintained. The cells on the sample wheel act as a level of containment and the PromISS 2 EXPERIMENT BOX prior to removal from the EXPERIMENT BOX. When the sample wheel is removed from the EXPERIMENT BOX the cells on the sample wheel and the Air Circulation supply two levels of containment. The Air Circulation is not turned off until the sample cells have been secured in the SAMPLE CONTAINER/RETURN CONTIANER and two levels of containment are in place with the cells and the SAMPLE CONTAINER/RETURN CONTAINER. 3. In the event of a water cooling loop failure, the Electronics Box may exceed touch temperature limit values. If water cooling loop failure is detected the MSG Facility will automatically power down the payloads and the operator must wait a minimum of 10 minutes before entering the WV. 4. The Electronics Box provides a High Voltage source to the Experiment Box, however the connector (J308) is inaccessible at all times after PromISS Activation, precluding a potential shock hazard to the operator. SAFETY NOTE MSG Facility Generic for BTF PROMISS Toxicity Level 444 Repeated 11 times 11 4

55. 55 PAYLOAD OPERATIONS/ PROCEDURE OVERVIEW (cont.) MGUEMSGPRS2N001PromISS 2 SETUP MGUEMSGPRS2N002PromISS 2 ACTIVATION MGUEMSGPRS2N003PromISS 2 STOW MGUEMSGPRS2N004PromISS 2 VIDEO OPERATIONS MGUEMSGPRS2R001 PromISS 2 REFERENCE DATA MGUEMSGPRS2R002 PromISS 2 OPERATIONS CONCEPT MGUEMSGPRS2C001 PromISS 2 CLEAN UP MGUEMSGPRS2C002 PromISS 2 SOFTWARE UPLOAD MGUEMSGPRS2ON001 PromISS 2 OPERATIONS NOMENCLATURE

56. 56 PromISS 2 MISSION SCENARIO PromISS 2 SETUP 75 Min Install PromISS 2 hardware EXPERIMENT BOX SAMPLE CONTAINER Mate POWER and DATA cable assemblies Install MSG Camera onto PromISS 2 Install PromISS 2 onto AL Lid AIR CIRC – ON (Mode 4) Remove SAMPLE CELLS from SAMPLE CONTAINER, install onto SAMPLE WHEEL and into PromISS 2 EXPERIMENT BOX Install PromISS 2 TAPE 1 PromISS 2 ACTIVATION 10 Min AIR CIRC – ON (Default MODE 4) Power On PWR OUT 2 +5V,  12V, +28V MSG VIDEO System* PromISS 3 PROCESSING Unattended 30 Days Once PromISS 2 is activated it will go to a “warm up” and run continuously The payload software will send commands to the MSG Video System PromISS 2 includes one MSG Camera mounted internally. Camera power is on for the entire processing time of 168 hours. AIR CIRC will be ON/OFF per concurrent SSM payload SETUP operations as timelined during all PromISS 2 Processing PromISS 2 STOW Power Off PWR OUT 2 +5V,  12V, +28V VIDEO System – STBY (remove TAPE 7) Remove Sample Wheel, remove SAMPLE CELLS place into RETURN CONTAINER AIR CIRC – OFF Remove, temporarily stow PromISS 2 hardware MSG MAIN PWR – STBY 55 Min ASSUMPTIONS 1.Hardware transfer completed, temporarily stowed 2.MSG Activated (MAIN PWR ON) 3.WV ILLUM – OFF 4.MSG VIDEO System – ON, (includes minimum power for; 1 CAMERA, 1 RECORDER, 2 MONITORS) Assumptions: 1, 2, 3 Assumptions: 2, 3, 4 Assumptions: 2, 3, 4, Assumptions: See instructions below PromISS 2 VIDEO OPERATIONS Repeated 11 times Exchange tapes 2 through 11, install 12 10 Min

57. 57 PAYLOAD DESCRIPTION Science Objective The monitoring of protein crystal growth in a three-dimensional environment to produce a detailed analysis and quantitative interpretation of the relationship between the quality of the crystals and the environment in which they were produced. The techniques allowing three-dimensional refocusing are mandatory to improve the accuracy of the concentration gradients. Two aspects considered for PromISS 3 are: -The convection motions around the crystal created by the gravity and the composition gradient have a direct impact on the crystal quality and on the concentration of impurities. There is a need to understand accurately these phenomena on the basis of three-dimensional experimental data. -The use of digital holographic monitoring is used to diagnose the locations, the shapes and the sizes of the growing crystals.

58. 58 SUMMARY The hardware consists of the following: –EXPERIMENT BOX –ELECTRONICS BOX –SAMPLE CONTAINER –RETURN CONTAINER –SAMPLE CELLS –SAMPLE WHEEL –LOCKING SYSTEM –CLAMP –SOFTWARE UPLOAD cable –TWEVLE VIDEO TAPES

59. 59 SUMMARY (cont.) PromISS 2 Operations: –Automated, two ground command –1 SAMPLE WHEEL, continuous processing –Utilize 1 MSG digital recorder, 1 MSG Video Camera, MLC