1. EDGE™ Final Project Plan Presentation P08043 – Mass Spectrometry Sample Preparation

2. EDGE™ Mass Spectrometry Project Plan Project Name Mass Spectrometry Sample Preparation Project Number P08043 Project Family Bioengineering Fundamentals Family of Projects Track Assistive Devices and Bioengineering Track Start Term 2007-2 End Term 2007-3 Faculty Guide Dr. Steven Day (ME) Confirmed Faculty Consultant Dr. Steve Weinstein (ME) Confirmed Primary Customer Dr. Steven Day (ME) Confirmed Secondary Customer Dr. Andy Hugh, St. Jude’s Children Hospital, Confirmed

3. EDGE™ Mission Statement Matrix Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI) is used to examine the tissue’s structure and properties. The methods in which tissues are coated affect the image quality and resolution. Experimenting different coating devices will help better understand the tissue coating dynamics.

4. EDGE™ Single-Ion MALDI Images: Mouse Embryo

5. EDGE™ Product Description The image quality of the MALDI drives the need to improve the coating process. Develop an experimental test rig that is robust and flexible. The design will consist of a coating device and a slide to measure the diffusion. The experimental device should be flexible to investigate different coating devices. Each coating device will apply an organic solvent to a diffusion slide. A high speed camera will allow for real time analysis of the coating process. This senior design project will provide the basic platform for a MS Thesis Project.

6. EDGE™ Coating Devices The experimental apparatus will allow for interchangeable coating devices. The coating devices will use a organic solvent. Three different coating devices will be constructed: 1) Airbrush 2) Modified Ink Jet Printer 3) Blade Coating A commercial ink jet and air brush will be purchased. The blade coating will be designed and fabricated using RIT’s CNC machines.

7. EDGE™ Staffing Requirements Project Manager (ME) - Project Manager: Responsible for coordinating all aspect of SDI and SDII. Will coordinate scheduling, team dynamics, research and development phases, design of experiments, and serve as a liaison between the senior design team and the customer(s). A strong understanding of the project scope and customer needs is critical. Research fluid mechanic properties related to ink jet printers, blade coating, and airbrush. Will help develop a diffusion slide and an integrated experimental apparatus to incorporate all three coating devices. ME Student 1 - Responsible for modifying the mechanical design of the ink jet printer. Develop a slide mounting, holding and registration of sample for ink jet printer. The ink jet printer design will attach to the experimental apparatus. Collaborating with the CE and EE students to integrate a GUI control system will be critical. A strong background in Statics/Mechanics/Dynamics is needed. A strong understanding of fluid mechanics/transport phenomena concepts and CAD is desired.

8. EDGE™ Staffing Requirements ME Student 1 - Responsible for researching different commercial blade coating designs. The ME student will propose and design a blade coating device to meet the customer's needs and objectives. Will coordinate the fabrication of the blade coating device using RIT's CNC machines. The blade coating design will attach to the experimental apparatus. Collaborating with the CE and EE students to integrate a GUI control system will be critical. Will work with the other team members to develop a diffusion slide. A strong background in Statics/Mechanics/Dynamics is needed. A strong understanding of fluid mechanics/transport phenomena concepts and CAD is desired. EE Student 1 - Responsible for developing a control and interface for both the ink jet printer and blade coating device. Must research and understand the electrical circuitry of ink jet printers. Will develop custom circuitry for the ink jet printer controls and diffusion slide. Work closely with the CE, IS, and ME students in development of a GUI and controls system to control the high speed camera, ink jet and blade coating device. Strong background on custom circuitry developments and programming is needed. Experience with robotics and LabView or related programs is desired.

9. EDGE™ Staffing Requirements CE Student 1 – The computer engineer will be responsible for control and interface with ink jet and blade coating device. Must research and understand the software/hardware components of the ink jet printer. Will be responsible for developing custom hardware and software to control the ink jet printer and blade coating device. Will work closely the EE, ME, and IS students in development of a GUI and controls system to control the high speed camera, ink jet and blade coating device. Experience in hardware and software development is needed. Strong background in different programming languages is desired. IE Student 1 – Responsible for packaging, user interface and system integration. Will help design and experimental apparatus that is user-friendly and efficient. The IE student will work closely with all team members to ensure the project objectives and goals satisfy the customer needs. Responsible for documenting/developing short-term and long-term project goals. Will develop a statistical analysis platform for the MS thesis project. A strong background in statistical analysis (Design of Experiments) is needed. A basic understanding of mechanical engineering concepts is desired.

10. EDGE™ Staffing Requirements IS Student 1 – The imaging arts and science major will be responsible for helping to develop and design a diffusion slide. The diffusion slide will have a known characteristic/pattern for all three coating devices. The imaging arts and science major will research the imaging and diffusion properties of the organic solvent. Will develop a model to measure the diffusion from all three devices. A strong background in printing instruments and material science is needed. An understanding of diffusion properties is desired.

11. EDGE™ Intellectual Property Considerations There are currently no intellectual property documents required for this senior design project.

12. EDGE™ Preliminary Work Breakdown Structure

13. EDGE™ Preliminary Work Breakdown Structure

14. EDGE™ Preliminary Work Breakdown Structure

15. EDGE™ Preliminary Work Breakdown Structure

16. EDGE™ Team Values and Norms All team member should share the same project vision and goals. Any concerns will be addressed as a team. Team members are expected to help one another and work collectively. All team members are expected to contribute in the decision making process (Fair Process), even if they are not actively involved in a particular process. Open-ended discussions and active problem solving will be encouraged. Team member’s roles and objectives should be clearly defined.

17. EDGE™ Grading and Assessment Scheme

18. EDGE™ Grading and Assessment Scheme

19. EDGE™ Grading and Assessment Scheme

20. EDGE™ Required Resources People 1.Dr. Day - Facilitate ongoing supervision of the project to ensure the goals and objectives are aligned with the customer’s needs. 2. Dr. Steve Weinstein – Provide technical expertise and advice with coating designs. 3.Dr. Andy High – Provide tissue samples, MALDI images and data. 4.Dr. Esterman – Serve as a liaison between the Imaging Science Department Environment 1. A controlled environmental chamber is needed to accurately test the device. Equipment 1.A low cost ink jet printer that will be used for the design project is needed. 2.A high speed camera to capture real time data coating process – Dr. Day will provide. 3.Polarized Optical Microscope (POM) to measure the optical properties. Microelectronic laboratory has a SEM and POM. Materials 1.Organic solvents (TBD) will need to be purchased to execute preliminary tests. Organic solvent must have similar viscosity and surface tension as customer’s solvent.

21. EDGE™ Mass Spectrometry Sample Preparation Constraints Materials Organic Solvent Tissue Properties Solvent Dye Humidity Diffusion Lateral Diffusion Matrix Crystallization Temperature Humidity Resources Equipments People Time Polarized Optical Microscope High Speed Camera Economics Budget Allowance SD I SD II Donations Control Chamber Scope Apply Organic Solvent Lead Time Customer Feedback User Friendly Package Integration Measure Diffusion Interviews Objectives /goals Customer Needs Big Picture Long/Short term planning Technology Spray Device Ink Jet Printer Blade Coating Air brush GUI Pressure Droplet Size Number of Layers Time

22. EDGE™ Identify Customer Needs - Benchmarking Primary Customer 1. Dr. Steven Day - Faculty Guide and customer. Secondary Customer 1.Dr. Andy High - St. Jude Children’s Hospital. Customer Constraints 1. Organic Solvent: 50/50 mixture of Acetynitrol and Water 2. Tissue Thickness: 10 – 15 μm. 3. Laser Size: 100 μm laser beam 4. Temperature and Humidity: Effects crystal growth and distribution 5. Proposed Budget: $5000 (confirmed)

23. EDGE™ Customer Needs

24. EDGE™ Customer Needs

25. EDGE™ Relative Importance of Customer Needs

26. EDGE™ Target Specifications

27. EDGE™ Target Specifications

28. EDGE™ Issues & Risks 1.Will organic solvent react with ink cartridge? 2.Measuring/characterizing diffusion 3.Integrated Experimental Apparatus

29. EDGE™ Outstanding Items - Future Plan 1.Follow-up with Dr. Esterman on potential organic solvents. 2.Follow-up with customer on proposed senior design solutions. 3.Continue to look potential students interested in this project.