1. aequatus-vis Anil Thanki Scientific Programmer – TGAC, UK @anilthanki @aequatus

2. RECAP Aequatus

3. Homology and Phylogeny ■Homology is existence of shared ancestry between a pair of structures, or genes, in different species. ■The phylogenetic information inferred from the study of homologous genes helps us to understand the evolution of gene families, @anilthanki

4. Aequatus ■Aequatus is an open-source web based tool to visualise homologous genes with phylogenetic tree. ■Aequatus reads data directly from Ensembl Compara and Ensembl Core Databases ■http://aequatus.tgac.ac.ukhttp://aequatus.tgac.ac.uk ■GitHub: https://github.com/TGAC/Aequatus-Browserhttps://github.com/TGAC/Aequatus-Browser @anilthanki

5. Aequatus @anilthanki monoamine oxidase

6. Aequatus Homologous Genes with shared exon information @anilthanki monoamine oxidase

7. Aequatus Gene Tree @anilthanki monoamine oxidase

8. Aequatus Reference information Controls @anilthanki monoamine oxidase

9. Reference information Aequatus @anilthanki Aequatus visualises similarity amongst homologous genes along with the event type responsible for existence of the gene, for example speciation, duplication, gene-split etc. Also indicates insertions and deletions With user-friendly interactions and viewing options monoamine oxidase

10. aequatus-vis

11. ■aequatus-vis is a JavaScript plugin to visualise homologous genes through alignment and phylogeny information. ■It preserves interactive functionality of Aequatus ■It has an ability to integration with countless web based applications @anilthanki

12. aequatus-vis 1.Gene Tree JSON / Newick 2.Gene structural Info JSON @anilthanki JSON format returned from e! server ■aequatus-vis takes gene tree and gene structural information as input ■Gene tree in either Newick or JSON from e! server ■Gene structures in JSON format from e! server

13. EXAMPLES

14. Aequatus @anilthanki Example of visualising homologous genes in Aequatus using aequauts-vis Next update

15. Aequatus e! Rest API @anilthanki Example of visualising homologous genes fetched from e! Rest API in Aequatus using aequatus-vis Testing

16. Galaxy @anilthanki Example of visualising homologous genes in Galaxy using aequatus-vis Pull request

17. TGAC Robert Davey Sarah Ayling Nicola Soranzo http://aequatus.tgac.ac.uk @anilthanki and @aequatus Contact Acknowledgements @anilthanki Javier Herrero UCL Cancer Institute, UK Matthieu Muffato EBI-EMBL, UK

18. Thank you ■Questions? ■Suggestions? ■Advice? ■Potential Users / collaborators ? @anilthanki