1. Lab 1: Introduction to Population Genetics August 22, 2012

2. Instructors uSteve DiFazio  5200 Life Sciences Building  Office Hours: Mon, Wed, Thurs 1 to 2 pm uHari Chhetri (TA)  5206 Life Sciences Building  Office Hours: Tue, Wed, Fri 11:30 to 12:30 uPlease use office hours, or make appointment!

3. Required Lab Manual Slavov, G, E. Rodgers- Melnick, and S.P. DiFazio. 2012. BIOL 464/GEN 535 Population Genetics Laboratory Manual. WVU Press. 107 Pages. uAvailable only from WVU book store (~$20) uPlease purchase by this Wednesday, August 22

4. Lab Reports uLab reports due at beginning of lab period  12 total, worth 10 points each  Deduct 0.5 points for each day late  Last report is optional: up to 10 pts extra credit uGuidelines for lab report are in lab manual uEmail and/or paper versions are fine uMost weeks require a write-up with interpretations for each calculation or simulation uPlease be careful to fully answer questions, including explanations of results from biological standpoint

5. Working Together and Academic Honesty uGroup work in laboratory is optional but encouraged uIt is fine to discuss lab problems and work on them together uHOWEVER, your lab report must be your original work  See academic honesty policy on class website uExams are based primarily on the lab exercises and examples worked in class

6. Highlights from the Schedule uIntroduction to Probability uGenetic variation in populations: Hardy-Weinberg Equilibrium uSelection uInbreeding uGenetic drift uGene Flow and population structure uPhylogenetics uGenetic identity and forensic identification uCoalescence and neutral theory uLinkage disequilibrium uQuantitative genetics uTying genotypes to phenotypes

7. Population Genetics is Important uDisease susceptibility, genetic testing, and personalized medicine uStatistical interpretation of forensic DNA evidence uHuman evolution and cultural history uCrop and animal improvement  Traditional breeding  Genetic engineering uConservation plans for plant and animal communities uResponses of plant and animal communities to climate change

8. Introductions Name Major Career goal/goal for class. Be honest!

9. Mathematical Tools for Population Genetics uBasic algebra uBasic calculus uBasic statistics uProbability

10. Population Genetics and Probability uProbability is at the core of much of population genetics  Reproduction is a sampling process  Effects of mutation, gene flow, selection, and genetic drift must be seen as departures from expectations based on random processes uExample: 1 genetic locus and two alleles in a forest of 20 trees determines color of foliage. Green is dominant.  What proportion of offspring will have white foliage? : 4 copies: 36 copies