1. 3D-Calculator1 Presentation of data analysis software for bone

2. 3D-Calculator2 The idea A software package that allows easy and complete analysis of bone scans. Standardization of parameter calculation Available to all research groups for free Possibility to add parameter plug-ins Allow batch processing

3. 3D-Calculator3 Software structure 1. Viewing of datasets (Tview / Histogram) 2. ROI/VOI selection 3. Segmenting of data sets 4. Calculate parameters (in a batch)

4. 3D-Calculator4 Implemented plug-ins Local threshold algorithm 2D Bone parameters (Parfitt’s model) Structure Model Index (SMI) 3D Direct Thickness Euler number (connectivity density) Mean Intercept Length - Degree of anisotropy

5. 3D-Calculator5 2D Parameters 2D-Parameters: Based on models (Parfitt’s plate model) and not as accurate as 3D- parameters. Trabecular thickness (Tb.Th.) Trabecular separation (Tb.Sp.) Trabecular number (Tb.N.) Bone volume/ Total volume (BV/TV)

6. 3D-Calculator6 2D Parameters

7. 3D-Calculator7 Structure Model Index (SMI) SMI gives information about the ‘curvature’ of the surface. (Hildebrand and Ruegsegger, 1997) Ideal objects: 0 = plate 3 = rod 4 = sphere

8. 3D-Calculator8 Direct Thickness 3D Direct thickness gives a volume weighted thickness distribution + average thickness (Hildebrand and Ruegsegger, 1997)

9. 3D-Calculator9 Connectivity Density Connectivity: Degree to which a structure is multiply connected. (Odgaard and Gundersen, 1993)

10. 3D-Calculator10 Mean Intercept Length (MIL) MIL can be used to get a measure of anisotropy in bone. (Whitehouse, 1974)

11. 3D-Calculator11 The software

12. 3D-Calculator12 Viewing data sets

13. 3D-Calculator13 ROI/VOI selection

14. 3D-Calculator14 Segmenting data sets

15. 3D-Calculator15 Batch processing