1. การยับยั้งจุลินทรีย์โดยใช้วัสดุซิลเวอร์นาโน ที่ได้จากเคมีสีเขียวเพื่อประยุกต์ใช้ในการ บำบัดน้ำ ( Antibacterial Ability of Ag and Ag/AgCl Nanoparticles from Radish and Tea Extracts for Water Treatment Application ) น. ส. ยุภาดา บุญโต ดร. จิรภัทร์ อนันตภัทรชัย, รศ. ดร. พวงรัตน์ ขจิตวิชยานุกูล

2. Change for safe the world

3. Comparisons of conventional method and green synthesis for produce green nanomaterials

4. Plant extract Green tea http://body-line.ro/img/cms/ceai-verde-in-compozitia-lipox-women.jpg http://upload.wikimedia.org/wikipedia/commons/ thumb/d/d8/Caffeine.svg/764px-Caffeine.svg.png Caffeine Radish http://chubeza.com/newsletter/wp-content/uploads/2010/11/radishes.jpg Folic acid http://en.wikipedia.org/wiki/Folic_acid

5. http://www.bluenanoinc.com/images/slv-np-100-nanoparticle-sem-image-2-large.jpg http://www.scworksthailand.com/nano/ Nano Technology

6. Silver NPs Bactericidal http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.s vc/ImageService/Articleimage/2014/CC/c4cc03001j/c4cc03001j-f1_hi-res.gif

7. Material and Method Radish juice DI water Blend (10min, 2 times) Tea extract DI water Boiled and Filtered Preparation of plant extract

8. Material and Method Synthesis Ag/AgCl-Radish (Ag/AgCl-R) 20 min AgNO 3 NaCl Radish Juice Ag

9. Synthesis Ag-Radish (Ag-R) Material and Method 15 min AgNO 3 Radish Juice Ag

10. Synthesis Ag-Tea (Ag-T) Material and Method AgNO 3 Mixing Settle at RT Tea extracted Ag

11. Result and Discussion Characterization of Ag NPs by SEM Ag/AgCl-R Ag-C Ag-R Ag-T

12. Result and Discussion Characterization of Ag NPs AgNP Crystal size (nm) Particle size (nm) pH pzc Ag/AgCl-R68.4±3.5188.1±17.92 Ag-R59.1±5.2180.3±12.42.7 Ag-T65.5±3.4382.6±18.22.8 Ag-C79.6±4.61637.1±23.13.1 XRD patterns of synthesized AgNP Crystal size, particle size and pH pzc of various types of AgNP Ag-T Ag/AgCl-R Ag-R Ag-C

13. LB agar 10 8 cfu/ml Incubate 37˚C 24 h Shaking at 250 rpm Bacteria in LB medium Incubate at 37˚C overnight CFU Ag NPs LB agar Agar disc diffusion test Inactivation test Bacteria culture E. coli S. aureus

14. Result and Discussion Agar disc diffusion test Ag/AgCl-R Ag-C Ag-T Control Ag-RControl Ag-R Ag/AgCl-R Ag-C Ag-T Control Ag-R Control Ag-R Ag/AgCl-R AgNP Inhibition zone diameter (mm) E. coliS. aureus Control (SDW)0.0±0.0 Ag-C0.0±0.0 Ag-T6.50±0.50 0.0±0.0 Ag-R8.75±0.25 Ag/AgCl-R8.75 ±0.759.50±0.10

15. Ag/AgCl-R Ag-R Ag-C Ag-T Result and Discussion Antibacterial activity of AgNP (100 µg/mL)

16. Result and Discussion Ag-T Ag/AgCl-R Ag-R Antibacterial activity (N/No) of AgNP on E. coli using different concentrations as a function of time

17. Result and Discussion AgNP Concentration of AgNP (µg/mL) Inactivation Rate (µg/mL-min) Antibacterial efficiency (%) Ag/AgCl-R 350.007222.3 700.001595.6 1500.004799.2 3000.0333100.0 Ag-R 350.002415.3 700.002580.5 1500.005491.4 3000.0250100.0 Ag-T 1500.004512.2 3000.007524.5 4500.003832.7 6000.007640.5 Comparison of inactivation rate and antibacterial efficiency of AgNP on E. coli using different concentration of AgNP

18. Conclusion The obtained AgNP from plant extracts were presented in a smaller size (good dispersion ability, less agglomeration) The AgNP from Radish extracted provided the highest percentage in inhibition of E. coli and S. aureus. The inactivation of E.coli using the AgNP was greater than that of S. aureus for all AgNP. The Ag/AgCl-R and Ag-R provided higher antibacterial activity more than Ag-T

19. Turning Your Lab into a Green Chemistry PLEASE