1. ADVANCED ANALYTICAL CHEMISTRY – CHEM 321 Merve Inal

2. Main Ideas/Goals 1. Instrument Components and Calibration: IS and Std Add 2. Signal to Noise 3. Introduction to Spectroscopy: UV-Vis, AE, Fluorescence, FTIR/IR, NMR 4. Introduction to Separations: GC, LC HPLC, MS 5. Good Lab Practice (GLP)

3. Instrument Components Energy Source (Stimulus) Analytical Information Information Sorter Input Transducer Data Domain of Transduced Information Signal Processor/Readout Device 1. What are the components of a pH meter?

4. Instrument Calibration Internal Standard: y = m  x

5. Instrumental Calibration Standard Addition: c x =b/m c x /S x = c s +c x /S (s+x)

6. Signal to Noise SSS Signal? Noise? Baseline? 1.Chemical Noise 2.Instrumental Noise: Thermal or Johnson noise  Shot noise  Flicker noise  Environmental noise How can we reduce the noise?

7. Introduction to Spectroscopy UV-Vis AE Fluorescence FTIR/IR NMR

8. Introduction to Separations GC HPLC MS

9. Good Lab Practice (GLP) Generate Lots of Paper “Raw data” in ink All data in laboratory workbooks, logbooks, or notebooks directly as it happens Date and sign or initial all entries Strike out changes in data entries with a single line with reasons provided, signed and dated If workbooks are shared among analysts, indicate which analyst performed what portion of the work Exact copy printouts should be the same as electronic version and all the changes made