1. Lab Equipment MicroscopeMicroscope Slide Cover SlipTest tube Test tube Rack Dissecting PanDissecting Scissors Dissecting PinsForceps PipetPetri DishWatch Glass Erlenmeyer FlaskBeaker Graduated Cylinder Magnifying Glass Safety Goggles

2. Warm-up: Spelling words copy the following terms 5 time each in your notebook Microscope Eyepiece Arm Stage Fine adjustment knob Course adjustment knob Objective Nose piece Diaphragm Body Tube Base

3. Types of Microscopes Light Microscope (Compound Microscope)‏ Can be used to view living objects. Natural Color and movement can be observed. Poor Resolution- 200nm Easy to use; fairly inexpensive Electron Microscope Objects can not be alive Black and white images with no movement Excellent resolution- 0.2nm Viewer must be highly trained; very expensive.

4. Compound Microscope vs. Electron Microscope

5. 2. Arm – provides support for the body tube 3. Eye piece – contains a lens that magnifies 4. Body Tube – allows light to pass up to the eye and provides the proper distance between the eyepiece lens and the objective lens. 5. Nose piece – holds the objectives and rotates to change magnification.

6. 6. Objective Lens 1. High-power obj. lens (longest) provides the highest magnification 2. Medium-power obj. lens 3. Low-power obj. lens (shortest) provides the lowest magnification 7. Stage Clips – holds microscope slide in place. 8. Stage opening – allows light to pass through the slide

7. 9. Stage – the platform where a slide is placed to be viewed 10. Diaphragm – regulates the amount of light 11. Light or mirror – provides or reflects light up through the specimen on the slide to the eye. 12. Base – supports the entire microscope 13. Fine adjustment Knob – used to bring the specimen into sharp focus 1. Coarse adjustment knob – used to focus the microscope

8. Magnification A light microscope has several lenses that magnify what you are viewing, which is why it is sometimes called a compound microscope. To find out how much magnification is being used, you first look at the eyepiece. It should be marked with a number such as 10x. This number means that the lens in the eyepiece magnifies 10 times. Now look at the low power objective. It is also marked with a number that tells its magnification. To find the total magnification being used, you multiply the two numbers.

9. Example1: The eyepiece of a light microscope is marked 10x and the low power objective is marked 4x. What is the total magnification? 10x * 4x = 40x

10. Example 2: The eyepiece of a light microscope is marked 20x and the low power objective is marked 5x. What is the total magnification? 20x * 5x = 100x

11. Example 3: The eyepiece of a light microscope is marked 100x and the low power objective is marked 60x. What is the total magnification? 100x * 60x = 6000x

12. Example 4: The eyepiece of a light microscope is marked 20x and the low power objective is marked 15x. What is the total magnification? 20x * 15x = 300x

13. Example 5: The eyepiece of a light microscope is marked 150 and the low power objective is marked 25x. What is the total magnification? 150x * 25x = 3750x