1. Lab-on-a-Chip The Ideal Technology for Bio-chemical Analysis
Xingyu Jiang Ph.D.
National Center for Nanoscience and Technology, China

2. What is “Lab-on-a-chip”?
A lab-on-a-chip (LOC) is a device that integrates one or
several laboratory functions on a single chip of only millimeters
to a few square centimeters in size.

3. What can “Lab-on-a-chip” do?
Biochemical assays: real-time PCR, immunoassay, dielectrophoresis for detecting cancer cells and bacteria, etc.
Chemical application: separating molecules from mixtures, chemical reactors, chemical detections. etc.
Biological application: cell coculture, biosensor, drug screening, single-cell analysis, etc.

4. Automation for Lab-on-a-chip
Development of Microfluidic Large Scale Integration with thousands of mechanical valves
Parallel Assays Made Easy!

5. Microfluidic Diagnostic Methods Portable Point-of-Care (POC) Medical Diagnostic Systems
The chips should be inexpensive, accurate, reliable and suited to the medical conditions in developing countries.

6. How to Prepare a Microfluidic Chip?
Photolithography
or etching
L-Edit, AutoCAD
Print
Soft embossing

7. The Method for Preparing a Chip in Our Lab: Soft embossing by polymer (PDMS etc.)

8. A method for analyzing HIV antibodies in microfluidic channels
PDMS:Polydimethylsiloxane
PC: Polycarbonates

9. Improved efficiency in assays
Antigens
Antibodies
Immunoassay results on PC membrane
Advantages:
Fast (20 min.)
High throughput
Low cost

10. Micro Western blot for detecting multiple proteins on one chip
Detection of multi-proteins one time
1-3µL Antibody solutions are required

11. Loop-mediated isothermal amplification (LAMP) integrated on microfluidic chips for detection of pathogen
Jiang, et al, Anal. Chem. 2010,82, 3002

12. Cells on a Chip

13. Microfluidic technology in cell coculture
We developed a method for patterning multiple types of adherent cells on the same substrate.

14. Naked-Eye Based Assays

15. Nanotechnology in chemical analysis