1. M.Sc. Andrés Ramírez Restrepo VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA

2. INDEX CONTEXT 1 METHODS & MATERIALS 2 RESULTS & DISCUSSION 3 CONCLUDING REMARKS 4 WHAT’S NEXT? 5 BIBLIOGRAPHY 6 ACKNOWLEDGEMENTS 7

3. CONTEXT

4. 4 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 1.1.0COLOMBIA https://www.muralesyvinilos.com/fotomurales/mapas/mapamundi-colores

5. 5 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 1.2.0COLOMBIAN PRODUCTS OIL COFFEE GOLD COAL FLOWERS EMERALDS www.colombiatrade.com.co www.colombia.travel FOODS

6. 6 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 1.3.0REGULATIONS To update this resolution, Agriculture Ministry and Social Protection Ministry will do a yearly update of the MRLs according to Codex Alimentarius.

7. 7 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 1.4.0PROBLEM Does Colombia has knowledge, equipment and know-how available to survey the presence of pesticide residues in foods according to Resolution 2906 of 2007? How do we combine all our capabilities to improve them and demonstrate the validity of our monitoring results? Will the information gathered allow the further issue of new and suitable MRLs in our country?

8. 8 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 1.5.0JUSTIFICATION PRACTICALMETHODREGULATORY

9. METHODS & MATERIALS

10. 10 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 2.1.0VALIDATION International Standards Organization ISO/IEC 17025:2005 «Confirmation by examination and the provision of objective evidence that the particular requirements for a specific intended use are fulfilled».

11. 11 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 2.1.1IMPLICATIONS VALIDATION AOAC 2007.01 EN 15662 Guide CG 4ISO/IEC 17025 12571/2013R-AC-01

12. 12 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 2.1.2CRITERIA SANCO/12571/2013 VALIDATION

13. 13 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 2.2.0FOODS Potato Solanum tuberosum Sugarcane Saccharum officinarum Goldenberry Physalis peruviana Lettuce Lactuca sativa Tamarillo Solanum betaceum Tomato Solanum lycopersicum

14. 14 Courtesy of M. Anastassiades

15. 15 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 2.3.0GDCON https://www.museodeantioquia.co/noticia/jornadas-para-reflexionar-el-centro-de-medellin/

16. 16 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 2.4.0INSTRUMENTS GC Agilent Technologies 7890A VL MSD Agilent Technologies 5975C Aquity UPLC H Class XEVO TQD Waters

17. 17 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 2.5.0RISK ISO/IEC 31000:2009 «Effect of uncertainty on objectives». International Standards Organization

18. 18 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 2.5.1SURVEY RISK http://www.uxabilidad.com/recursos/mapa-politico-de-colombia-en-vectores.html

19. 19 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 2.5.1SURVEY RISK http://pharus.webnode.es/turismo-sostenible/

20. RESULTS & DISCUSSION

21. 21 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 3.1.0EXTRACTS

22. 22 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 3.2.1SELECTIVITY EVIDENCE

23. 23 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 3.2.2MATRIX EFFECT EVIDENCE Y AXIS X AXIS

24. 24 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 3.2.3PRECISION EVIDENCE

25. 25 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 3.2.4TRUENESS EVIDENCE

26. 26 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 3.2.5UNCERTAINTY EVIDENCE

27. 27 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 3.3.0VALIDATION SUMMARY

28. 28 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 3.4.0RISK MAP Significant chronic toxicity risk in tomatoes!

29. 29 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 3.5.0ACCREDITATION 13-LAB-053

30. CONCLUDING REMARKS

31. 31 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 4.0.0CONCLUDING REMARKS By means of QuEChERS and chromatographic techniques (GC-MS and UPLC-MS/MS) it was possible to provide objective evidence of the fullfilment of the requirements. There is a gap between local and international know-how. Its causes should be found and attended to improve Colombian installed capacity. The survey suggests that risks from pesticides should be attended.

32. WHAT’S NEXT?

33. 33 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 5.0.0WHAT’S NEXT? More pesticides, more foods, more risk assessment. National scale survey. Update Colombian Resolution.

34. BIBLIOGRAPHY

35. 35 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 6.0.0BIBLIOGRAPHY x37 x20 x19 Anastassiades, Lehotay, et al. EURLs Datapool ISO Res. 2906

36. ACKNOWLEDGEMENTS

37. 37 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 7.0.0ACKNOWLEDGEMENTS

38. ANDRÉS RAMÍREZ RESTREPO Calle 62 No. 52-59 Torre 2. Laboratorio 232 Sede de Investigación Universitaria – SIU Universidad de Antioquia. Medellín, Antioquia quimico.andres@gmail.com Tel. +57 4 2630791 – 57 4 2196571 +57 300 567 4727

39. THANK YOU FOR YOUR KIND ATTENTION ¡MUCHAS GRACIAS POR SU ATENCIÓN!