1. Life at the Edge The plasma membrane is the boundary that separates the living cell from its nonliving surroundings The plasma membrane exhibits selective permeability, allowing some substances to cross it more easily than others

2. Figure 5.1 © 2014 Pearson Education, Inc.

3. Cellular membranes are fluid mosaics of lipids and proteins Phospholipids are the most abundant lipid in the plasma membrane Phospholipids are amphipathic molecules, containing hydrophobic and hydrophilic regions The fluid mosaic model states that a membrane is a fluid structure with a “mosaic” of various proteins embedded in it

4. Hydrophilic head Hydrophobic tail WATER

5. Hydrophilic region of protein Hydrophobic region of protein Phospholipid bilayer

6. The Fluidity of Membranes Phospholipids in the plasma membrane can move within the bilayer Most of the lipids, and some proteins, drift laterally Rarely does a molecule flip-flop transversely across the membrane

7. Lateral movement (~10 7 times per second) Flip-flop (~ once per month) Movement of phospholipids

8. As temperatures cool, membranes switch from a fluid state to a solid state The temperature at which a membrane solidifies depends on the types of lipids Membranes rich in unsaturated fatty acids are more fluid than those rich in saturated fatty acids Membranes must be fluid to work properly; they are usually about as fluid as salad oil

9. Fluid Unsaturated tails prevent packing. Cholesterol Viscous Saturated tails pack together. (a) Unsaturated versus saturated hydrocarbon tails (b) Cholesterol reduces membrane fluidity at moderate temperatures, but at low temperatures hinders solidification.

10. Viscous Fluid Unsaturated hydrocarbon tails with kinks Membrane fluidity Saturated hydro- carbon tails

11. The steroid cholesterol has different effects on membrane fluidity at different temperatures At warm temperatures (such as 37°C), cholesterol restrains movement of phospholipids At cool temperatures, it maintains fluidity by preventing tight packing

12. Cholesterol Cholesterol within the animal cell membrane

13. Some proteins in the plasma membrane can drift within the bilayer Proteins are much larger than lipids and move more slowly

14. Membrane proteins Mixed proteins after 1 hour Hybrid cell Human cell Mouse cell

15. Membrane Proteins and Their Functions A membrane is a collage of different proteins embedded in the fluid matrix of the lipid bilayer Proteins determine most of the membrane’s specific functions Peripheral proteins are not embedded Integral proteins penetrate the hydrophobic core and often span the membrane

16. Fibers of extracellular matrix (ECM) Glycoprotein Carbohydrate Microfilaments of cytoskeleton Cholesterol Integral protein Peripheral proteins CYTOPLASMIC SIDE OF MEMBRANE EXTRACELLULAR SIDE OF MEMBRANE Glycolipid

17. Integral proteins that span the membrane are called transmembrane proteins The hydrophobic regions of an integral protein consist of one or more stretches of nonpolar amino acids, often coiled into alpha helices

18. EXTRACELLULAR SIDE N-terminus C-terminus CYTOPLASMIC SIDE  Helix

19. Six major functions of membrane proteins: – Transport – Enzymatic activity – Signal transduction – Cell-cell recognition – Intercellular joining – Attachment to the cytoskeleton and extracellular matrix (ECM)

20. Enzymes Signal Receptor ATP Transport Enzymatic activity Signal transduction

21. Glyco- protein Cell-cell recognition Intercellular joining Attachment to the cytoskeleton and extra- cellular matrix (ECM)

22. The Role of Membrane Carbohydrates in Cell-Cell Recognition Cells recognize each other by binding to surface molecules, often carbohydrates, on the plasma membrane Membrane carbohydrates may be covalently bonded to lipids (forming glycolipids) or more commonly to proteins (forming glycoproteins) Carbohydrates on the external side of the plasma membrane vary among species, individuals, and even cell types in an individual

23. Synthesis and Sidedness of Membranes Membranes have distinct inside and outside faces The asymmetrical distribution of proteins, lipids and associated carbohydrates in the plasma membrane is determined when the membrane is built by the ER and Golgi apparatus

24. Plasma membrane: Cytoplasmic face Extracellular face Transmembrane glycoprotein Plasma membrane: Secreted protein Vesicle Golgi apparatus Glycolipid Secretory protein Transmembrane glycoproteins ER

25. Membrane structure results in selective permeability A cell must exchange materials with its surroundings, a process controlled by the plasma membrane Plasma membranes are selectively permeable, regulating the cell’s molecular traffic

26. The Permeability of the Lipid Bilayer Hydrophobic (nonpolar) molecules, such as hydrocarbons, can dissolve in the lipid bilayer and pass through the membrane rapidly Polar molecules, such as sugars, do not cross the membrane easily

27. Transport Proteins Transport proteins allow passage of hydrophilic substances across the membrane Some transport proteins, called channel proteins, have a hydrophilic channel that certain molecules or ions can use as a tunnel Channel proteins called aquaporins facilitate the passage of water

28. Other transport proteins, called carrier proteins, bind to molecules and change shape to shuttle them across the membrane A transport protein is specific for the substance it moves

29. Passive transport is diffusion of a substance across a membrane with no energy investment Diffusion is the tendency for molecules to spread out evenly into the available space Although each molecule moves randomly, diffusion of a population of molecules may exhibit a net movement in one direction At dynamic equilibrium, as many molecules cross one way as cross in the other direction

30. Molecules of dyeMembrane (cross section) WATER Net diffusion Equilibrium Diffusion of one solute

31. Substances diffuse down their concentration gradient, the difference in concentration of a substance from one area to another No work must be done to move substances down the concentration gradient The diffusion of a substance across a biological membrane is passive transport because it requires no energy from the cell to make it happen

32. Net diffusion Equilibrium Diffusion of two solutes Net diffusion Equilibrium

33. Effects of Osmosis on Water Balance Osmosis is the diffusion of water across a selectively permeable membrane The direction of osmosis is determined only by a difference in total solute concentration Water diffuses across a membrane from the region of lower solute concentration to the region of higher solute concentration

34. Lower concentration of solute (sugar) Higher concentration of sugar Same concentration of sugar Selectively permeable mem- brane: sugar mole- cules cannot pass through pores, but water molecules can H2OH2O Osmosis

35. Water Balance of Cells Without Walls Tonicity is the ability of a solution to cause a cell to gain or lose water Isotonic solution: solute concentration is the same as that inside the cell; no net water movement across the plasma membrane Hypertonic solution: solute concentration is greater than that inside the cell; cell loses water Hypotonic solution: solute concentration is less than that inside the cell; cell gains water

36. Animals and other organisms without rigid cell walls have osmotic problems in either a hypertonic or hypotonic environment To maintain their internal environment, such organisms must have adaptations for osmoregulation, the control of water balance The protist Paramecium, which is hypertonic to its pond water environment, has a contractile vacuole that acts as a pump

37. Filling vacuole 50 µm Contracting vacuole

38. Water Balance of Cells with Walls Cell walls help maintain water balance A plant cell in a hypotonic solution swells until the wall opposes uptake; the cell is now turgid (firm) If a plant cell and its surroundings are isotonic, there is no net movement of water into the cell; the cell becomes flaccid (limp), and the plant may wilt In a hypertonic environment, plant cells lose water; eventually, the membrane pulls away from the wall, a usually lethal effect called plasmolysis

39. Animal cell Lysed H2OH2O H2OH2O H2OH2O Normal Hypotonic solution Isotonic solutionHypertonic solution H2OH2O Shriveled H2OH2O H2OH2O H2OH2O H2OH2O Plant cell Turgid (normal) FlaccidPlasmolyzed

40. Facilitated Diffusion: Passive Transport Aided by Proteins In facilitated diffusion, transport proteins speed movement of molecules across the plasma membrane Channel proteins provide corridors that allow a specific molecule or ion to cross the membrane Carrier proteins undergo a subtle change in shape that translocates the solute-binding site across the membrane

41. EXTRACELLULAR FLUID Channel protein Solute CYTOPLASM

42. Carrier protein Solute

43. Active transport uses energy to move solutes against their gradients Facilitated diffusion is still passive because the solute moves down its concentration gradient Some transport proteins, however, can move solutes against their concentration gradients

44. The Need for Energy in Active Transport Active transport moves substances against their concentration gradient Active transport requires energy, usually in the form of ATP Active transport is performed by specific proteins embedded in the membranes The sodium-potassium pump is one type of active transport system

45. Cytoplasmic Na + bonds to the sodium-potassium pump CYTOPLASM Na + [Na + ] low [K + ] high Na + EXTRACELLULAR FLUID [Na + ] high [K + ] low Na + ATP ADP P Na + binding stimulates phosphorylation by ATP. Na + K+K+ Phosphorylation causes the protein to change its conformation, expelling Na + to the outside. P Extracellular K + binds to the protein, triggering release of the phosphate group. P P Loss of the phosphate restores the protein’s original conformation. K + is released and Na + sites are receptive again; the cycle repeats. K+K+ K+K+ K+K+ K+K+ K+K+

46. Diffusion Facilitated diffusion Passive transport ATP Active transport

47. Maintenance of Membrane Potential by Ion Pumps Membrane potential is the voltage difference across a membrane Two combined forces, collectively called the electrochemical gradient, drive the diffusion of ions across a membrane: – A chemical force (the ion’s concentration gradient) – An electrical force (the effect of the membrane potential on the ion’s movement)

48. An electrogenic pump is a transport protein that generates the voltage across a membrane The main electrogenic pump of plants, fungi, and bacteria is a proton pump

49. H+H+ ATP CYTOPLASM EXTRACELLULAR FLUID Proton pump H+H+ H+H+ H+H+ H+H+ H+H+ + + + + + – – – – –

50. Cotransport: Coupled Transport by a Membrane Protein Cotransport occurs when active transport of a solute indirectly drives transport of another solute Plants commonly use the gradient of hydrogen ions generated by proton pumps to drive active transport of nutrients into the cell

51. H+H+ ATP Proton pump Sucrose-H + cotransporter Diffusion of H + Sucrose H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ + + + + + + – – – – – –

52. Bulk transport across the plasma membrane occurs by exocytosis and endocytosis Small molecules and water enter or leave the cell through the lipid bilayer or by transport proteins Large molecules, such as polysaccharides and proteins, cross the membrane via vesicles

53. Exocytosis In exocytosis, transport vesicles migrate to the membrane, fuse with it, and release their contents Many secretory cells use exocytosis to export their products

54. Endocytosis In endocytosis, the cell takes in macromolecules by forming vesicles from the plasma membrane Endocytosis is a reversal of exocytosis, involving different proteins

55. Three types of endocytosis: – Phagocytosis (“cellular eating”): Cell engulfs particle in a vacuole – Pinocytosis (“cellular drinking”): Cell creates vesicle around fluid – Receptor-mediated endocytosis: Binding of ligands to receptors triggers vesicle formation

56. Phagocytosis Food vacuole “Food” or other particle CYTOPLASM Pseudopodium Solutes EXTRACELLULAR FLUID Pseudopodium of amoeba An amoeba engulfing a bacterium via phagocytosis (TEM) Bacterium Food vacuole 1  m

57. Pinocytosis Plasma membrane Coat protein Coated pit Coated vesicle Pinocytotic vesicles forming (TEMs) 0.25  m

58. Top: A coated pit Bottom: A coated vesicle forming during receptor- mediated endocytosis (TEMs) 0.25  m Receptor-Mediated Endocytosis Receptor Plasma membrane Coat protein

59. Animations and Videos How Diffusion Works Diffusion Osmosis Bozeman - Osmosis Demo Bozeman - Diffusion Demo Plasmolysis Hemolysis and Crenation Contractile Vacuole

60. Animations and Videos Bozeman - Water Potential How Facilitated Diffusion Works Sodium-Potassium Pump Exchange Bozeman - Transport Across Cell Membrane Cotransport Proton Pump Amoeboid Movement Second Messengers (cAMP and Ca+2 Pathways)Second Messengers (cAMP and Ca+2 Pathways)

61. Animations and Videos Chemical Synapse – 1 Chemical Synapse – 2 Voltage-Gated Channels and the Action PotentialVoltage-Gated Channels and the Action Potential Clathrin-Coated Pits and Vesicles Receptors Linked to a Protein Channel Passive Transport Active Transport by Group Translocation

62. Animations and Videos Secondary Active Transport Organization of the Golgi Antiport Uniport...Carrier Protein Gated and Non-gated Channels Symport Cellulose Synthesis during Elongation Signal Transduction Pathway

63. Animations and Videos Signaling by Secreted Molecules Signal Transduction 2nd Messenger Signal Amplification – 1 Signal Amplification – 2 Cotranslational Targeting of Secretory Proteins to the ERCotranslational Targeting of Secretory Proteins to the ER Mechanism of Tyrosine Kinase

64. Animations and Videos Chapter Quiz Questions – 1 Chapter Quiz Questions - 2