1. Biochemical Reactions The Active Cell

2. Biological Reactions Metabolism is a term used to refer to all the chemical reactions that take place in a living organism Two main categories of metabolic reactions –Catabolic reactions are those that break large molecules into small ones E.g. –digestion of proteins –respiration –Anabolic reactions are those that build larger molecules E.g. photosynthesis

3. Metabolism Summary

4. Gibbs Free Energy and Metabolism Gibbs free energy measures the likelihood that a reaction will proceed spontaneously A calculation based on the energy required to break the bonds between the atoms of the reactants as compared to the products

5. Catabolic reactions Most catabolic reactions release energy This energy is available to do work (physical definition of work) These types of reactions are called exothermic in chemistry – energy is released at once in the form of heat E a = activation energy ΔG = Gibbs Free Energy

6. Catabolic Reactions In biological system, a sudden release of heat energy in the living cell will cause it to die Therefore, energy is released in a controlled manner through a variety of chemical intermediates Thus, in biology, we call these exergonic reactions

7. Anabolic Reactions These are chemical reactions that result in larger molecules Usually require a large energy input In chemistry, these are called endothermic reactions – energy input in the form of heat

8. Anabolic Reactions As with catabolic reactions, these process are highly controlled in the living cell –Cannot add energy directly by heating the reactants –Must do so in small controlled amounts These types of reactions are called endergonic

9. Reaction Energy Summary

10. ATP – Energy Molecule of the Cell

11. Enzymes Enzymes are functional proteins in that they are the main chemical drivers of life These are protein molecules that accelerate and control biochemical reactions by reducing the activation energy of a reaction The organic equivalent of a catalyst

12. Catalysis Refers to the process by which a chemical reaction proceeds with the assistance of a helper compound or molecule The helper is not consumed in the reaction General form is: Reactants + Catalyst Reactants/Catalyst complex Products + Catalyst

13. Enzymes Similar pathway for reactivity Reactants are called substrates Substrates + Enzyme Activated Enzyme Substrate complex Products + Enzyme

14. Enzymes As with catalysts, enzymes reduce the amount of activation energy needed to start a reaction. The energy curve of an enzyme mediated reaction will have a lower activation energy than that without the enzyme

15. Enzymes Note that ΔG for an enzyme mediated reaction does not change Only the activation energy is changed – reduced by a factor Enzymes work in one of two ways –Catabolic enzymes have an active site that will place stress on a specific bond causing it to break –Anabolic enzymes have an active site that will bring two molecules together in a configuration that permits bonding

16. Enzyme Action

17. Controlling Enzyme Activity Enzymes are reusable Active enzymes will continue to function as long as substrate is available to react Often, it is essential to control when an enzyme is active and when it is not Such controls are important in controlling how and when biochemical reactions take place

18. Controlling Enzyme Activity Several methods by which enzyme action is controlled Co-Enzymes and Co-Factors –These are molecules or minerals that are essential for the enzyme to function properly Inhibitors –These are molecules that interfere with the functioning of the enzyme –Two types Competitive inhibitors Allosteric inhibitors Biochemical Pathways

19. Co-Enzymes and Co-Factors

20. Enzyme Inhibitors -

21. Enzyme Inhibitors - Applications

22. Metabolic Pathways