1. P2HRI Wellesbourne lBrian Thomas lLol Trueman lBrian Smith P3University of Liverpool Hamish Collin Meriel Jones Brian Tomsett GARLIC & HEALTH WP4Garlic Sulphur Biochemistry

2. lTo establish the limiting biochemical and physiological steps for allicin formation in garlic lBiochemical pathway lIntegration with development lGenes for limiting steps GARLIC & HEALTH Garlic Sulphur Biochemistry

3. GARLIC & HEALTH Pathway Biochemistry

4. GARLIC & HEALTH Pathway Biochemistry (II) CSO 3 Alliinase Pyruvate Ammonia S alk(en)yl sulfenic acids Allicin Organosulphur compounds Tissue damage

5. GARLIC & HEALTH Integration with development

6. lRadiolabelled intermediates identified using HPLC and HPLC-MS in comparison with chemically synthesised compounds (P3). lDifferential display sequences and isolated and reanalysed by northern blots and in situ hybridisation, where possible, with other Allium sp. (P3) lFirst year harvests and analysis completed (P2). lSecond year experimental material planted. (P2). lcDNA clones encoding alliinase isolated and sequenced (P2). GARLIC & HEALTH Second Year Milestones

7. lDP.16: Pathway intermediates identified (P2, P3) lDP.17: First sulphur budget for garlic (P2) lDP. 18: Clones for alliinase GARLIC & HEALTH Second Year Deliverables