1. Chemistry of Cigarette Smoke Define secondhand smoke. Chem. Catalyst-Day 2

2. Exposure to these chemicals occurs whenever a tobacco product is burned. Tobacco smoke contains more than 4,000 compounds. some are carcinogenic: arsenic (lung cancer), benzene (leukemia), vinyl chloride (liver, brain cancer) β-napthalamine & 4- aminobiphenyl (bladder cancer) Fatemeh Mizbani The Firsthand Exposure

3. Experimentation Experimentation and Methods Sample collection in the field or at smoking chamber, using various devices such as TP-ETS sampler Identification of compounds using Gas Chromatography NPD or GC-Ion trap mass-mass spectroscopy Analysis using data and chemistry knowledge of the scientists.

4. Gas chromatography (GC) Gas chromatography (GC), is a common type of chromatography used in analytic chemistry for separating and analyzing compounds that can be vaporized without decompositionchromatographyanalytic chemistryseparatingvaporizeddecomposition GC-IT-MS-MS Spectrometer GC-NPD

5. Collecting SHS Using Thermopheric Deposition 500mm  T = 1600 °K cm -1 22°C 100°C Heater heat sink Interaction between heated gas molecules and SHS (or any small airborne particulate matter) results in a force toward the cooler side and deposition. TP- ETS Sampler Fatemeh Mizbani

6. Analysis of Secondhand Smoke – SHS  SHS gases and particles behave differently after smoking.  SHS particles (inside the yellow circle) are oily liquid aerosols that contain thousands of compounds  Nicotine is found in SHS particles but at much lower concentrations than in the gas phase Polycyclic Aromatic Hydrocarbons Particle- phase Benzene CO 1,3-Butadiene Formaldehyde Acrolein NO 2 Nicotine PAH Solanesol Benzo(a)pyrene Metals

7. Identification of Gaseous Compounds in SHS

8. Homework: Collecting THS You will be provided with kim-wipes and a vial of water and soap in a sealed plastic bag. To activate Kim-wipe moisten with soap and water. Wipe a surface exposed to thirdhand smoke with the moist Kim-wipe. Put it back in the sealed bag for tomorrow’s analysis.