1. Effect of Cultured Autologous Chondrocytes on Repair of Chondral Defects in a Canine Model* by HOWARD A. BREINAN, TOM MINAS, HU-PING HSU, STEFAN NEHRER, CLEMENT B. SLEDGE, and MYRON SPECTOR J Bone Joint Surg Am Volume 79(10):1439-51 October 1, 1997 ©1997 by The Journal of Bone and Joint Surgery, Inc.

2. Figs. 1-A through 1-D: Light micrographs of specimens from the fresh, untreated defects. HOWARD A. BREINAN et al. J Bone Joint Surg Am 1997;79:1439-51 ©1997 by The Journal of Bone and Joint Surgery, Inc.

3. Fig 1-B Specimen from one of two defects that had injury through the calcified cartilage caused by the curet. HOWARD A. BREINAN et al. J Bone Joint Surg Am 1997;79:1439-51 ©1997 by The Journal of Bone and Joint Surgery, Inc.

4. Fig. 1-C Specimen from a defect in which the most residual cartilage is in a corner and there is a corner fracture (open arrow) through the calcified cartilage. HOWARD A. BREINAN et al. J Bone Joint Surg Am 1997;79:1439-51 ©1997 by The Journal of Bone and Joint Surgery, Inc.

5. Fig. 1-D Specimen from a defect from which virtually all articular cartilage had been removed to the level of the tidemark. HOWARD A. BREINAN et al. J Bone Joint Surg Am 1997;79:1439-51 ©1997 by The Journal of Bone and Joint Surgery, Inc.

6. Fig. 2 Light micrograph of reparative tissue from three specimens that had safranin-O and fast- green staining after eighteen months of healing. HOWARD A. BREINAN et al. J Bone Joint Surg Am 1997;79:1439-51 ©1997 by The Journal of Bone and Joint Surgery, Inc.

7. Fig. 3 Light micrograph showing reparative tissue in the center eighteen months after implantation of cultured autologous chondrocytes (trichrome). HOWARD A. BREINAN et al. J Bone Joint Surg Am 1997;79:1439-51 ©1997 by The Journal of Bone and Joint Surgery, Inc.

8. Fig. 4 Light micrograph (the same specimen as in Fig. 2, B) showing reparative tissue at the edge of a defect eighteen months after coverage with periosteum alone (trichrome). HOWARD A. BREINAN et al. J Bone Joint Surg Am 1997;79:1439-51 ©1997 by The Journal of Bone and Joint Surgery, Inc.

9. Fig. 5 Light micrograph showing reparative tissue at the edge of the specimen eighteen months after creation of a defect that was left untreated. HOWARD A. BREINAN et al. J Bone Joint Surg Am 1997;79:1439-51 ©1997 by The Journal of Bone and Joint Surgery, Inc.

10. Figs. 6-A, 6-B, and 6-C: Light micrographs showing relatively poor repair (safranin O-fast green) after eighteen months of healing. HOWARD A. BREINAN et al. J Bone Joint Surg Am 1997;79:1439-51 ©1997 by The Journal of Bone and Joint Surgery, Inc.

11. Fig. 6-B Specimen from a defect that was covered with periosteum alone. HOWARD A. BREINAN et al. J Bone Joint Surg Am 1997;79:1439-51 ©1997 by The Journal of Bone and Joint Surgery, Inc.

12. Fig. 6-C Specimen from an empty defect. HOWARD A. BREINAN et al. J Bone Joint Surg Am 1997;79:1439-51 ©1997 by The Journal of Bone and Joint Surgery, Inc.

13. Fig. 7 Bar graph showing the composition of the tissue found in the full-thickness defects as a function of time and treatment. HOWARD A. BREINAN et al. J Bone Joint Surg Am 1997;79:1439-51 ©1997 by The Journal of Bone and Joint Surgery, Inc.