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Published byFlorence Cole Modified over 8 years ago
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Advanced Confocal Microendoscopy Wibool Piyawattanametha, Ph.D.
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High resolution subsurface imaging RESOLUTION (log) IMAGE PENETRATION (log) 1 mm 100 m 10 m 1 m ULTRASOUND MRI, CT OPTICAL COHERENCE TOMOGRAPHY 0 100 m 1 mm 1 cm 10 cm OPTICAL COHERENCE MICROSCOPY CONFOCAL MICROSCOPY TWO-PHOTON MICROSCOPY DUAL-AXES CONFOCAL MICROSCOPY
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What are we trying to achieve? Provide optical sectioning with histopathology resolution with molecular contrast in real time Benefits: Cancer screening (sub-surface imaging): -Early detection of cancer -Aid tumor staging diagnosis Locate tumor margin/boundary Track targeted therapy etc.
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“Virtual biopsy” with miniature microendoscope Handheldminiaturized 1 cm Zeiss ® 1 m Tabletop
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Cancers of epithelium Malignant tissues originating from epithelial layer of organs -Epithelial layer: 0-700 µm from the tissue surface Extremely common cancer: -85% of all new cancer cases Extremely deadly: -80% of all cancer deaths Examples: ovary, cervix, upper and lower GI, ENT, brain, bladder, etc.
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10-mm intravital & skin scope (785-nm) 5-mm GI endoscope (785-nm) 1 cm Confocal microscope & endoscope 10-mm5-mm 5 cm
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4 m MEMS scanner Microelectromechanical systems (MEMS) based microendoscope (5-mm diameter)
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Assembly of a 5-mm diameter microendoscope
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Ex vivo imaging of human colon Crypt lumens Colonocyte Normal Adenomatous polyps Scale bars: 100 µm
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Normal squamous esophagus biopsy Vertical Section Horizontal Section (275 m deep)
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Barrett’s esophagus biopsy Vertical Section Horizontal Section (200 m deep)
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Real-time in vivo tumor vasculature 100 µm
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Mosaicing of real-time in vivo human skin
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Real-time in vivo imaging of human colon
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wibool@gmail.com
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