1. The AfCS Antibody Database Goal: –provide the signaling community with a resource on the AfCS Antibody Lab’s experience with commercial antibodies. Content: –information on over 300 antibody preparations, most of which are phosphorylation site specific (up from 200 last year) –additional testing of antibodies on RAW 264.7 cells Format: –Last year unveiled in PDF format –This year it is web-based with search engine added

2. Location of Database on the Web 1.Go to AfCS website at: www.signaling-gateway.org www.signaling-gateway.org 2.Click on 3.Links to the database and the brief communication Report explains how to navigate database and how we scored the performance of the antibodies

3. Navigating the Antibody Database The database is organized into three levels: Level 1: Index of antibody targets Level 2: List of antibodies to chosen target Level 3: Table of results for chosen antibody Search engine available to refine search at any level

4. Level 1: Index of Antibody Targets Only small portion of index shown Example: click on p38 MAP kinase

5. Level 2: List of Antibodies to Chosen Target Lower the number score, the better the antibody performed for us Click on name of antibody preparation

6. Ab name & source Each row represents a single experiment Columns give information on conditions and results of each experiment (different conditions can result in different score). Color-coding that corresponds to the scoring of results are defined in Brief Communication and our poster. Lowest number scores (best results) are always listed at top. Level 3: Summary Table of Results

7. Search Engine Categories available to refine search: - Vendor - Score - Phosphospecific - Cell type - Cell treatment

8. AfCS Antibody Database: New Web-based Version Becky Fulin, Lonnie Sorrells, Robert Sinkovits*, Ruth Levitz, and Heping Han The Alliance for Cellular Signaling: The Antibody Laboratory (University of Texas Medical Center at Dallas) and the Bioinformatics and Data Coordination Laboratory (University of California at San Diego)* The AfCS Antibody Database Learn about Scoring and More from Poster # 20 Acknowledgements: Technical Staff, Antibody and Cell Preparation and Analysis Laboratories Robert Sinkovits, Bioinformatics and Data Coordination Laboratory Lonnie Sorrells, Data Manager of the Dallas AfCS Laboratories Becky Fulin and Ruth Levitz, Antibody Laboratory Susanne Mumby, Director of the Antibody Laboratory