1. Date of download: 5/31/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Anti-CD3/Anti-CD28 Bead Stimulation Overcomes CD3 Unresponsiveness in Patients With Head and Neck Squamous Cell Carcinoma Arch Otolaryngol Head Neck Surg. 2000;126(4):473-479. doi:10.1001/archotol.126.4.473 Anti-CD3 stimulation of lymph node mononuclear cells. Proliferative responses were significantly higher in responders (P=.001 for day 4; P=.002 for day 6). 3 H indicates tritium. Figure Legend:

2. Date of download: 5/31/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Anti-CD3/Anti-CD28 Bead Stimulation Overcomes CD3 Unresponsiveness in Patients With Head and Neck Squamous Cell Carcinoma Arch Otolaryngol Head Neck Surg. 2000;126(4):473-479. doi:10.1001/archotol.126.4.473 Anti-CD3 stimulation of peripheral blood mononuclear cells. Proliferative responses were significantly higher in responders (P=.008 for day 4; P=.01 for day 6). 3 H indicates tritium. Figure Legend:

3. Date of download: 5/31/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Anti-CD3/Anti-CD28 Bead Stimulation Overcomes CD3 Unresponsiveness in Patients With Head and Neck Squamous Cell Carcinoma Arch Otolaryngol Head Neck Surg. 2000;126(4):473-479. doi:10.1001/archotol.126.4.473 Anti-CD3/anti-CD28 bead stimulation of lymph node mononuclear cells. Proliferative response was significantly higher in responders on day 4 (P=.02, t test). No difference was seen on day 6. 3 H indicates tritium. Figure Legend:

4. Date of download: 5/31/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Anti-CD3/Anti-CD28 Bead Stimulation Overcomes CD3 Unresponsiveness in Patients With Head and Neck Squamous Cell Carcinoma Arch Otolaryngol Head Neck Surg. 2000;126(4):473-479. doi:10.1001/archotol.126.4.473 Anti-CD3/anti-CD28 bead stimulation of peripheral blood mononuclear cells. No significant differences were seen between groups on day 4 or 6. 3 H indicates tritium. Figure Legend:

5. Date of download: 5/31/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Anti-CD3/Anti-CD28 Bead Stimulation Overcomes CD3 Unresponsiveness in Patients With Head and Neck Squamous Cell Carcinoma Arch Otolaryngol Head Neck Surg. 2000;126(4):473-479. doi:10.1001/archotol.126.4.473 Results of 18-hour chromium 51 release assay of anti-CD3/anti-CD28 bead-stimulated peripheral blood mononuclear cells (PBMCs) and lymph node mononuclear cells (LNMCs) vs autologous tumor in responder 11. For all experiments, SD was 1%. Figure Legend:

6. Date of download: 5/31/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Anti-CD3/Anti-CD28 Bead Stimulation Overcomes CD3 Unresponsiveness in Patients With Head and Neck Squamous Cell Carcinoma Arch Otolaryngol Head Neck Surg. 2000;126(4):473-479. doi:10.1001/archotol.126.4.473 Results of 18-hour chromium 51 release assay of anti-CD3/anti-CD28 bead-stimulated peripheral blood mononuclear cells (PBMCs), lymph node mononuclear cells (LNMCs), and healthy control PBMCs from responder 11 vs allogeneic tumor in nonresponder 5. For all experiments, SD was 1%. Figure Legend: