1. Fat and Breast Cancer Mei-Chun Chen, MD 1,2 ; Hsu Ma, PhD 1 Taipei Veterans General Hospital, Taiwan 1.Division of Plastic and Reconstructive Surgery, Department of Surgery, Taipei Veterans General Hospital,Taipei City, Taiwan 2.Taipei Municipal Gan-Dau Hospital, Taipei City, Taiwan Disclusore status : Nothing to disclose

2. Autologous fat transfer in breast cancer Improved the cosmetic outcome after breast- conservative surgery.

3. Tumor microenvironment Nat. Rev. Cancer, 2009, 9, 239-252 Tumor cell Pro-inflammatory cytokines Non-malignant cellular components

4. Objectives The response of breast cancer cells to fat. Radiation Fat graft Breast cancer cells Progression ? Regression ?

5. Materials and Methods Cell line: MDA-MB-231 and MCF-7 4 groups: Control Adipose-derived stem cell Fat tissue (liposuction) Adipocytes (from fat tissue) Cytokines: IL-6, IL-8, IGF-1, FGF-7, RANTES Check survival viability of the breast cancer cells with transwell co-culture methods and different cytokines.

6. SRB assay MDA-MB-231 cells which were co-culture with adipocyte increased 50% cell growth.

7. Cell Count More cell count of MDA-MB-231 cells which were co-culture with adipocyte.

8. MDA-MB-231 (400X) Control With stem cell With fat tissue With adipocyte

9. SRB assay MDA-MB-231 10% FBS - DMEMSerum free - DMEM N = 3

10. IL-8 10% FBS - DMEMSerum free - DMEM

11. IGF-1 Serum free - DMEM10% FBS - DMEM * * * * * *

12. FGF-21 10% FBS - DMEM Serum free - DMEM *

13. RANTES 10% FBS - DMEM Serum free - DMEM *

14. Conclusion MDA-MB-231 cells which were co-culture with adipocyte increased 50% cell growth. More cell count of MDA-MB-231 cells which were co-culture with adipocyte. IGF-1 mildly increases the growth of MDA-MB- 231 cells. Fat tissue that was harvested by liposuction does not significantly increase the growth of MDA-MB- 231.

15. Thanks for your attention! Taipei Veterans General Hospital, Taiwan