1. http://www.itrainsfishes.net/content/biotopes/mangrove.php

2.  Aromatase is an enzyme critical in sexual determination  Converts androgen to estrogen  Conserved across all vertebrates and mammals  Located in the brain, ovary, and testes  This study assesses protein alignment and evolutionary relationships between Kmar and other Teleosts

3.  Obtained sequence from NCBI ◦ Taxonomy report ◦ Saved in FASTA format  Put sequences in SEAVIEW ◦ Converted to Protein ◦ Aligned Protein Sequence  Used RaxML ◦ Determined Bootstrap values  Input into Figtree ◦ Graphical representation of evolutionary relationship

4. Protein Alignment

5. Aromatase Tree

6. Concatenated Tree

7.  Similar protein structure when Kmar compared to other Teleosts  Teleosts evolved at similar rates  Kmar and Medaka closely related ◦ Evolved at similar evolutionary rates  Out-group evolved separately