1. Palindrome, Restriction Enzyme, Sticky Ends GAATTC G AATTC G Sticky Ends (Cohesive Ends) EcoRI CIVIC, Madam Get An Apple To The Class G AATTCG Arber, Nathans, Smith (1978) Juang RH (2004) BCbasics

2. Restriction Mapping of DNA A B 10 kb 8 kb 2 kb A 7 kb 3 kb B 5 kb 3 kb 2 kb A+BA+B CK A B A+B M Restriction enzymes Juang RH (2004) BCbasics

3. The Specific Cutting and Ligation of DNA GAATTC CTTAAG GAATTC CTTAAG G CTTAA AATTC G G G CTTAA G AATTC G G CTTAA AATTC G G CTTAA AATTC G EcoRI DNA Ligase EcoRI sticky end Juang RH (2004) BCbasics