1. Algae Rules Algae Rules Biofuel Engineering Team: Greg Rowe, James Song, Eric Stephanson, and James Zajac.

2. PERSONNEL INFO Dr. Gregory Rowe The product of 4 billion years of evolution. alumnus of the University of Washington Electrical Engineering department. 6 years of programming experience and 3 years of experience with advanced robotics. He once applied to work on a directed evolution project. Dr. James Song An alumnus from the University of Washington Chemical Engineering department Earned a PhD in Chemical Engineering at the age of 8. Has 11 years of experience with process design and computational fuel extraction methods extensive experience in the biofuel industry should prove essential to the goals of Algae Rules. Dr. Eric Stephanson An alumnus from the University of Washington Electrical Engineering department. Currently has 7 years of experience with electromagnetics 5 years experience testing high voltage fields on bacteria cells for gene splicing. He amazingly built a Tesla coil in his garage at the age of 14. Dr. James Zajac An alumnus from the University of Washington Mechanical Engineering department, Has 18 years of experience with DNA splicing and 5 years of experience with current algae biofuel extraction methods. His direct experience with algae will prove useful for Team Algae Rules.

3. Overview Problem Background Solution Timeline Cost of project Future projections

4. CURRENT PROBLEMS Current lipid extraction methods from algae Requires heavy mechanical and hazardous chemical Harm to environment, costly, time consuming Not economically feasible enough to return investments Fig. 1: Expensive equipment Fig. 2: Hazardous chemicals

5. Fig. 3: Brand new more feasible single-step process

6. Algae Biodiesel Type of water-growing plant cultivated for use as a fuel. high content of oil, clean burning, renewable, no petroleum. Rapid growth and quick repopulation. re-grow much quicker than other oil-producing crops. Plant is harvested and undergoes processing to extract the oil. Table 1: Yield of common crops

7. Fig. 4: CO 2 recovery process

8. ALGAE RULES HAS THE SOLUTION Develop a strain of algae that more efficiently produces lipids. More biofuel-quality lipids per algae production cycle investment. Algae produces inter-cellular lipids that can be processed into valuable biofuels.

9. STATEMENT OF WORK Utilize the MAGE process/Evolution Machine to modify algae DNA. Develop an algae strain that produces lipids 2x more efficiently than dunaliella tertiolecta. Classify proposed super-strain as D. Extrimisawesomus.

10. MAGE PROCESS Fig. 5: MAGE process schematic

11. D. Extrimisawesomus PERFORMANCE SCHEMATIC

12. PROJECT TIMELINE Project Duration: ~8 months

13. PROJECTED COSTS ItemEstimated Cost DNA Synthesizer $40,000 Micro Bioreactors$10,000 Electroporator $7,000 Algae Growth Medium $1,000 Electrical/Mechanical Automation $10,000 Assembly & Maintenance$5,000 Total Cost: ~$473,000 Total Labor (~8 months) $400,000 Table 2: MAGE process schematic

14. FUTURE IMPLICATIONS Greatly increased feasibility of processing valuable biofuels from algae. The possibility of replacing fossil fuels with algae biofuels. Strengthening of domestic and international energy sectors. Energy security for the future.

15. Q&A SESSION