1. Pyramid Lake Paiute Tribe Bioassessment Program Technical Issues Daniel Mosley Environmental/ WQ Specialist Pyramid Lake Paiute Tribe

2. Truckee River Basin

3. Pyramid Lake

4. Pyramid Lake, NV

5. Truckee River

6. Stone Mother

7. CUI – UI (Kwee-wee)

8. Lahontan Cutthroat Trout

9. 27 pounder

10. Bio-Criteria n WQCP: Narrative Standards –Species Composition Communities and populations of aquatic biota, including invertebrate, vertebrate and plant species, shall not be degraded as a result of point source or nonpoint source discharge. This applies to transient as well as cumulative conditions. Short-term variances from these objectives may be allowed for actions that are being taken to fulfill statutory requirements under Tribal law or the federal Endangered Species Act.

11. Reference Sites

12. Reference Condition Process –1981. -LTR comprehensive survey. –1989 -Began using EPA RBA protocols. –1998 -Began attending workshops/ trainings, and develop working relationships with other agancies. –2002 -Began evaluating LTR BMI data.

13. Biotic Index ValuesBiotic Index Values n 0.0 – 3.5Excellent (Pristine water) n 3.5 – 4.5Very Good Water Quality n 4.5 – 5.5Good WQ (some pollution) n 5.5 – 7.0Fair (Moderately polluted) n 7.0 – 8.0Poor (seriously polluted) n 8.5 – 10.0Very Poor (extremely polluted waters)

14. Identifying Stressors: nPoint source: Waste Water Treatment Plant nNonpoint Sources: North-Tahoe Drain, Steamboat Creek, urban storm water runoff, and Irrigation return flows back into the river. nExotic Species: Non-native salmonids, like Rainbow trout, compete and hybridize with native Lahontan Cutthroat trout (LCT). Brown and Rainbow trout are more aggressive, and effectively compete with LCT. Tall Whitetop, purpleloose strife, and the aquatic Eurasian watermilfoil are competing with native plants.

15. Year Sampling Method Counting Method 1981SurberTotal 1982-1988 NO DATA 1989SurberTotal 1990SurberTotal 1991 1992KicknetEstimated 1993KicknetEstimated 1994SurberPresence/Absence 1995SurberPresence/Absence 1996SurberTotal 1997 1998 1999SurberTotal 2000SurberTotal

16. NW MBD WB DO FJR I80 LNX NB ND NIX SS AH CN Scattered

17. The PLPT Biological Index consists of 4 core metrics: Taxa Richness Percent EPT Individuals Percent Tolerant Individuals Percent Dominant Taxon

18. Approximat e Upstream Distance (miles) from MBD Station ID YearAverage 1981198919901996199920002001* 22.5I8063.639.343.647.051.248.9 20WB18.735.846.533.7 19AH47.051.249.1 17.5FJR52.825.159.451.247.1 14.5SS47.347.3 11.5NW38.980.859.9 10.5DO60.539.059.352.745.9*51.5 8CN34.250.551.745.547.9*45.9 6.5ND40.140.1 5LNX73.835.355.154.1*54.6 3.5NB28.363.345.8 2NIX71.045.457.650.1*56.1 0MBD58.216.344.745.525.638.1 Average59.227.745.251.347.350.751.247.5 *Only 2 of 6 sites for 2001 had been processed and ready for this analysis. BOLD sites are sites that will be part of the future sampling scheme.100-80Excellent69-60Good49-40Poor79-70 Very Good 59-50Fair39-0 Very Poor PLPT Biological Index Score and Narrative Rating

19. Spatial and Temporal Trends

21. Taxa Frequency

22. Mule DeerMule DeerMule DeerMule Deer