1. In silico feedback for in vivo regulation of a gene expression circuit Shared by lxshen

2. Biological feedback Signals Biological control circuits Implement of control

3. Comparison of control circuits Synthetic biological circuits Nature circuits robust Difficulty in artificial circuits Not easy to control slow Electronic circuits Prosperous Easy to control More functions Fast

4. Interface

5. model Fourth-order linear ordinary differential equation Identification experiment to determine five parameters fluorescence delay (fluorescence ≠ gene expression)

6. Applying model Ⅰ Task: increasing fluorescence 4 or 7 times over a 7-h period Open-loop(OL)control Fail to regulate fluorescence to desired levels due to inaccuracies of the model and something unpredictable

7. Applying model Ⅱ Closed-loop(CL) feedback control Kalman filter

8. Significance More predictable and accurate Avoid the disadvantage of bio-circuits Facilitate synthetic systems

9. Q ﹠ A