1. Teo et al. Figure S1a lox66 cre SacI-cre-F PCR, SacI/EcoRI cleavage and ligation to SpeI-lox66-SacI adapter T4 DNA ligase-mediated ligation cre EcoRI-cre-R SacIEcoRI SpeI/EcoRI cleavage pUBI-AB 4745 bp ProUBI NOSTer ProUBI NOSTer pUBI-AB-Cre 5799 bp pUBI-AB 4745 bp cre lox66 ColE1 Amp ColE1 SpeI EcoRI

2. Teo et al. Figure S1b T4 DNA ligase-mediated ligation SfiI cleavage pUBI-AB-Cre 5799 bp p6d35S 9820 bp p6d35S-Cre5 12662 bp Prod35S 35STer ProUBI NOSTer Prod35S 35STer NOSTer ProUBI ColE1 RB SfiI HPT LB Sm/Sp pVS1 ORI lox66 cre HPT LB Sm/Sp pVS1 ORI Amp ColE1 SfiI ColE1 RB

3. Teo et al. Figure S1c SmaI HpyCH4V 1x pAtT4 telomeric block SmaI/HpyCH4V cleavage T4 DNA ligase-mediated ligation StuI cleavage SmaI HpyCH4V p6d35S-Cre5 12662 bp p6d35S-Cre5-TEL7 13078 bp Prod35S 35STer Prod35S 35STer NOSTer ProUBI NOSTer HPT cre lox66 RB ColE1 pVS1 ORI Sm/Sp LB StuI HPT LB Sm/Sp pVS1 ORI ColE1 RB 1x pAtT4 telomeric block lox66 cre

4. Teo et al. Figure S1d AfeI HpyCH4V 1x pAtT4 telomeric block AfeI/HpyCH4V cleavage T4 DNA ligase-mediated ligation AfeI HpyCH4V AfeI cleavage p6d35S-Cre5-TEL7 13078 bp p6d35S-Cre5-2XTEL37 13402 bp Prod35S 35STer NOSTer ProUBI Prod35S 35STer NOSTer ProUBI pVS1 ORI Sm/Sp LB HPT cre lox66 AfeI RB ColE1 1x pAtT4 telomeric block lox66 cre HPT LB Sm/Sp pVS1 ORI ColE1 RB 1x pAtT4 telomeric block