1. Compound Light Microscope
Becky Sheridan

2. Compound (2) Light Microscope
Two Lenses
Objective Lens
Ocular/Eyepiece Lens
Total Magnification
Both lenses multiplied
Multiply the Objective Lens (#) x Eyepiece Lens (#) = Total Magnification
Example: x 10 = 100TM

3. What does the microscope do to an image? “e”
Makes it bigger
Turns it upside down
Turns it backwards
Shows more detail
Low Power
High Power

4. What happens when you move an object under the microscope?
Away
Towards you
Left
Right
Move toward you
Moves away
Moves right
Moves left
For a tutorial on the Compound Light Microscope
Author's): Jen Klipfel, Nabila Jahchan, Peggy Klipfel LeDuff
Go to:

5. VS Resolution The ability to see two items as separate discrete units.
Magnification
The size of an image

7. http://www. sciencelearn. org

8. Refraction and Immersion Oil
Refraction: the bending of light as it passes from one medium to another of different density. This is due to differences in their index of refraction.
Ex. Light passing through a glass microscope slide, air and then lens is refracted each time. This may result in blurring of the image when using the oil immersion lens.
Immersion Oil: the refractive index of immersion oil is about which is close to that of glass

9. Refractive Index of Glass & Immersion Oil
Refractive Index of Glass & Immersion Oil. The glass rod placed in immersion oil is practically invisible while the rod in water is visible.
Immersion oil has the same refractive index as glass, therefore light passing thru the oil & glass is not bent concealing the surface of the glass.
Immersion Oil
Water

10. Stereomicroscope Dissecting microscope

11. Stereomicroscope Dissecting microscope
A microscope with a relatively low magnification.
Good for viewing large objects
Produces a 3-D image
Advantages:
it has a large depth of field, so you can observe thick objects with most parts in focus at the same time.
can illuminate your sample using incident light as well as transmitted light, so that thick objects which will not transmit much light can be illuminated adequately to view.

12. Stereomicroscope
the stage is much farther from the objective lens, allowing large objects to be placed on the stage.
Hornet

13. Electron Microscopes
Use a beam of electrons as the source of illumination allowing much greater magnification and resolution than a compound microscope.
Transmission electron microscopes (TEM) transmit electrons through a thin slice of sample. Can view things like organelles in a cell.
Scanning electron microscopes (SEM) use incident electrons on whole samples to get a 3-dimensional image.
Images are viewed through an eyepiece, or projected onto a screen or photographic plate

14. TEM
SEM
Baby Bee
Cell

15. TEM
SEM