1. Chapter 7 Cell Structure and function

2.  Introduction to Cells Introduction to Cells

3.  Cells are the simplest collection of matter that can live.  Cells were first observed by Robert Hooke in 1665.  Working with more refined lenses, Antoni van Leeuwenhoek later described blood, sperm, and organisms living in pond water. © 2012 Pearson Education, Inc.

4. Introduction  Since the days of Hooke and Leeuwenhoek, improved microscopes have vastly expanded our view of the cell. © 2012 Pearson Education, Inc.

5. Exploring the Cell © 2012 Pearson Education, Inc.

6. A variety of microscopes have been developed for a clearer view of cells and cellular structure. The most frequently used microscope is the light microscope (LM)—like the one used in biology laboratories. ◦ Light passes through a specimen, then through glass lenses, and finally light is projected into the viewer’s eye. ◦ Specimens can be magnified up to 1,000 times the actual size of the specimen. © 2012 Pearsn Education, Inc.

7.  Beginning in the 1950s, scientists started using a very powerful microscope called the electron microscope (EM) to view the ultrastructure of cells. Instead of light, EM uses a beam of electrons.  Electron microscopes can resolve biological structures as small as 2 nanometers and magnify up to 100,000 times. © 2012 Pearson Education, Inc.

8.  Scanning electron microscopes (SEM) study the detailed architecture of cell surfaces.  Transmission electron microscopes (TEM) study the details of internal cell structure. © 2012 Pearson Education, Inc.

9. Figure 4.1C

10. Prokaryotes and Eukaryotes  Cells vary is size from 5-50 micrometers  What is the largest?  Smallest?

11.  Smallest Bacteria found n the ocean that is 400 micrometers  Largest In humans – nerves cells (close to 5 feet) Other: giant squid (almost 40 feet)

12. Prokaryotes Bacteria and archaea are prokaryotic cells. ◦ Plasma membrane ◦ One or more chromosomes ◦ Ribosomes ◦ no true organelles. © 2012 Pearson Education, Inc.

13. Eukaryotes  All other forms of life are composed of eukaryotic cells. ○ a plasma membrane ○ one or more chromosomes ○ ribosomes. ○ membrane-bound nucleus ○ number of other organelles.

14.  Almost all of the organelles and other structures of animals cells are present in plant cells. A few exceptions exist. Lysosomes and centrioles are not found in plant cells. Plant but not animal cells have ○ a rigid cell wall, ○ chloroplasts, and ○ a central vacuole. © 2012 Pearson Education, Inc.

16. THE NUCLEUS AND RIBOSOMES © 2012 Pearson Education, Inc.

17. Figure 4.4A Smooth endoplasmic reticulum Rough endoplasmic reticulum NUCLEUS: Nuclear envelope Chromatin Nucleolus Ribosomes Golgi apparatus Mitochondrion Plasma membrane Peroxisome CYTOSKELETON: Microtubule Intermediate filament Microfilament Lysosome Centriole NOT IN MOST PLANT CELLS:

18. Figure 4.4B NUCLEUS: Nuclear envelope Chromatin Nucleolus Golgi apparatus Rough endoplasmic reticulum Ribosomes Peroxisome Central vacuole NOT IN ANIMAL CELLS: Chloroplast Cell wall Plasmodesma Mitochondrion Plasma membrane Cell wall of adjacent cell Smooth endoplasmic reticulum CYTOSKELETON: Microtubule Intermediate filament Microfilament

19. The nucleus is the cell’s genetic control center  The nucleus contains most of the cell’s DNA and controls the cell’s activities by directing protein synthesis by making messenger RNA (mRNA).  © 2012 Pearson Education, Inc.

20.  The nuclear envelope is a double membrane and has pores that allow material to flow in and out of the nucleus. © 2012 Pearson Education, Inc.

21.  The nucleolus is a prominent structure in the nucleus and the site of ribosomal RNA (rRNA) synthesis. © 2012 Pearson Education, Inc.

22. Two membranes of nuclear envelope Nucleus Chromatin Nucleolus Pore Endoplasmic reticulum Ribosomes

23.  Ribosomes are involved in the cell’s protein synthesis. Ribosomes are synthesized from rRNA produced in the nucleolus. Cells that must synthesize large amounts of protein have a large number of ribosomes. © 2012 Pearson Education, Inc.

24. Free ribosomes are ○ involved in making proteins that function within the cytoplasm. Bound ribosomes are ○ attached to the endoplasmic reticulum (ER) © 2012 Pearson Education, Inc.

25. Ribosomes ER Cytoplasm Endoplasmic reticulum (ER) Free ribosomes Bound ribosomes Diagram of a ribosome Protein mRNA Colorized TEM showing ER and ribosomes

26. THE ENDOMEMBRANE SYSTEM © 2012 Pearson Education, Inc.

27.  The endomembrane system includes the nuclear envelope, endoplasmic reticulum (ER), Golgi apparatus, lysosomes, vacuoles, and the plasma membrane. © 2012 Pearson Education, Inc.

28. The endoplasmic reticulum is a biosynthetic factory  There are two kinds of endoplasmic reticulum—smooth and rough. Smooth ER lacks attached ribosomes. Rough ER lines the outer surface of membranes. Although physically interconnected, smooth and rough ER differ in structure and function. © 2012 Pearson Education, Inc.

29. Smooth ER Rough ER Ribosomes Nuclear envelope

30. Transport vesicle buds off mRNA Ribosome Polypeptide Glycoprotein Rough ER Sugar chain Secretory protein inside trans- port vesicle 4 3 2 1

31.  Smooth ER is involved in a variety of diverse metabolic processes. Smooth ER produces enzymes important in the synthesis of lipids, oils, phospholipids, and steroids. Other enzymes help process drugs, alcohol, and other potentially harmful substances. Some smooth ER helps store calcium ions. © 2012 Pearson Education, Inc.

32.  Rough ER Makes proteins © 2012 Pearson Education, Inc.

33.  The Golgi apparatus serves as a molecular warehouse and finishing factory for products manufactured by the ER. Products travel in transport vesicles from the ER to the Golgi apparatus. One side of the Golgi apparatus functions as a receiving dock for the product and the other as a shipping dock. Products are modified as they go from one side of the Golgi apparatus to the other and travel in vesicles to other sites. © 2012 Pearson Education, Inc.

34. Golgi apparatus Transport vesicle from the Golgi “Shipping” side of Golgi apparatus Transport vesicle from ER “Receiving” side of Golgi apparatus 1 2 3 4 4

35. A lysosome is a membranous sac containing digestive enzymes. ◦ The enzymes and membrane are produced by the ER and transferred to the Golgi apparatus for processing. ◦ The membrane serves to safely isolate these potent enzymes from the rest of the cell. © 2012 Pearson Education, Inc.

36.  Lysosomes help digest food particles engulfed by a cell. 1. A food vacuole binds with a lysosome. 2. The enzymes in the lysosome digest the food. 3. The nutrients are then released into the cell. © 2012 Pearson Education, Inc.

37. Digestive enzymes Lysosome Plasma membrane

38. Digestive enzymes Lysosome Food vacuole Plasma membrane

39. Digestive enzymes Lysosome Food vacuole Plasma membrane

40. Figure 4.10A_s4 Digestive enzymes Lysosome Food vacuole Plasma membrane Digestion

41.  Lysosomes also help remove or recycle damaged parts of a cell. 1. The damaged organelle is first enclosed in a membrane vesicle. 2. Then a lysosome ○ fuses with the vesicle, ○ dismantles its contents, and ○ breaks down the damaged organelle. © 2012 Pearson Education, Inc.

42. Animation: Lysosome Formation Right click on animation / Click play

43. Lysosome Vesicle containing damaged mitochondrion

44. Lysosome Vesicle containing damaged mitochondrion

45. Lysosome Vesicle containing damaged mitochondrion Digestion

46.  Vacuoles are large vesicles that have a variety of functions. In plants, vacuoles may ○ have digestive functions, ○ contain pigments, or ○ contain poisons that protect the plant. © 2012 Pearson Education, Inc.

47. Contractile vacuoles Nucleus

48. Central vacuole Chloroplast Nucleus

49. Figure 4.12 Smooth ER Nucleus Transport vesicle from ER to Golgi Golgi apparatus LysosomeVacuolePlasma membrane Nuclear membrane Rough ER Transport vesicle from Golgi to plasma membrane

50. ENERGY-CONVERTING ORGANELLES © 2012 Pearson Education, Inc.

51.  Mitochondria  are organelles that carry out cellular respiration in nearly all eukaryotic cells.  Cellular respiration converts the chemical energy in foods to chemical energy in ATP (adenosine triphosphate). © 2012 Pearson Education, Inc.

52. Figure 4.13 Matrix Cristae Inner membrane Outer membrane Mitochondrion Intermembrane space

53.  Chloroplasts are the photosynthesizing organelles of all photosynthesizing eukaryotes. Converts light energy into the chemical energy of sugar molecules. © 2012 Pearson Education, Inc.

54. Figure 4.14 Inner and outer membranes Granum Stroma Chloroplast Thylakoid

55. THE CYTOSKELETON AND CELL SURFACES © 2012 Pearson Education, Inc.

56.  Cytoskeleton which functions in structural support and motility. © 2012 Pearson Education, Inc.

57. Figure 4.16 Actin subunit Nucleus Microfilament Intermediate filament Fibrous subunits 7 nm 10 nm Tubulin subunits Microtubule 25 nm

58. While some protists have flagella and cilia that are important in locomotion, some cells of multicellular organisms have them for different reasons. ◦ Cells that sweep mucus out of our lungs have cilia. ◦ Animal sperm are flagellated. © 2012 Pearson Education, Inc.

59. Figure 4.17A Cilia

60. Figure 4.17B Flagellum

61. Figure 4.17C Outer microtubule doublet Central microtubules Radial spoke Dynein proteins Plasma membrane

62. © 2012 Pearson Education, Inc. Animation: Cilia and Flagella Right click on animation / Click play

63.  A plant cell, but not an animal cell, has a rigid cell wall that protects and provides skeletal support that helps keep the plant upright against gravity and is primarily composed of cellulose. . © 2012 Pearson Education, Inc.

64. Figure.21 Vacuole Plant cell walls Plasmodesmata Cytoplasm Primary cell wall Secondary cell wall Plasma membrane