1. Catalyst: Journal Write Please write at least ONE full paragraph (minimum of five sentences), being sure to answer all of the following questions.  What items in your everyday lives are dyed?  Why is dye important?  What are some ways that you think dyes can connect to chemistry?

2. Studying Zebra Fish (Danio rerio) embryos http://speakingofresearch.files.wordpress.com/2011/02/zebrafish.jpg http://www.swarthmore.edu/NatSci/sgilber1/DB_lab/Fish/imgs/epiboly.gif http://cdn1.iconfinder.com/data/icons/PLASTICXP/medical/png/400/microscope.png http://www.malariaworld.org/sites/default/files/sun.jpg http://school.discoveryeducation.com/clipart/images/sm-camera.gif

3. Examples of Fixing and Staining http://www.botanicalpaperworks.com/uploads/ck/images/PinkEgg_Crayon.jpg http://liks.dsvshop.com/uploads/831bc55572/ca372523d4.jpg http://upload.wikimedia.org/wikipedia/commons/thumb/0/02/TieDyeShirtMpegMan.jpg/220px-TieDyeShirtMpegMan.jpg http://www.dailycognition.com/content/image/3/blueman4-paul-karason.jpg http://image.made-in-china.com/2f0j00bBFTGnzlAvci/Invisible-Ink-Pen-with-Mini-UV-Light.jpg http://2007.igem.org/wiki/index.php/Image:Fluorescence.jpg

4. pH and Indicators Day 2 http://www.chagrinvalleysoapandsalve.com/idascorner/images/pHcolor.gif http://www.quickmeme.com/meme/36boyq/ Learning Target: I can create three natural dye solutions – one acidic dye, one basic dye and one neutral dye. I can determine the pH of a dye using pH indicators. I can calculate the molarity and pH of each dye solution.

5. What is pH?  pH measures how acidic or basic a substance is.  The range of pH is from 1 (very acidic) 14 (very basic).  A substance with a pH of 1-6 is acidic.  Ex: lemon juice, stomach acid, vinegar  A substance with a pH of 8-14 is basic.  Ex: baking soda, antacids (tums), household cleaners  A substance with a pH of 7 is neutral. http://www.ducksters.com/science/acids_and_bases.php

6. What are pH indicators?  Detects hydronium (H 3 O + ) or hydrogen (H + ) ions in a solution  Causes a color change depending on the pH  Color change occurs over a narrow range. http://ilovebacteria.com/Images/cabbage.jpg http://www.sciencephoto.com/image/4433/530wm/A5000509- Universal_indicator_papers-SPL.jpg Phenolphtalein http://www.all-about-ph.com/ph-indicators.html

7. How do pH, indicators, acids, bases and natural dyes connect?  The color of some natural dyes can be adjusted using NaHCO 3 (baking soda) and C 2 H 4 O 2 (vinegar).  Natural dyes can also be fixed by using mordants.  Made from salts or hydroxides of aluminums or chlorides.  Examples of Mordants: Alum, Cream of Tartar, etc. http://blog.clareflorist.co.uk/flowers/2012/04/tips-for- tuesday-natural-dyes-are-all-around-us-.html

8. Some Helpful Reminders  Molarity = moles of solute / liters of solution  pH = - log 10 [H 3 O + ]  pOH = - log 10 [OH - ]  [H 3 O + ] [OH - ] = 1.0 x 10 -14  pH + pOH = 14 http://serc.carleton.edu/images/mathyouneed/swirl _math.jpg