1. NI LabVIEW TM Laboratory Virtual Instrument Engineering Workbench

2. LabVIEW  Graphical language by icon & wire  Consist of front Panel & diagram  Virtual instrument  Interpreter language  Fast development speed  Low execution speed

3. LabVIEW environment Front panelBlock diagram

4. Basic syntax  Control, indicator, constant  Loop (for, while, case, sequence)  Array & clustor  Functions (sub vi) for loop Random # generator File IO function Array

5. SUB VI’s  For easy to debug and maintain  Reuse the same code in different situations

6. Serial port communication  Communicate with only one device  Very slow  For Long distance  For low data transfer rates

7. TCSPC for scanning mode Main control panel TCSPC Initializing Measure Data processing monocrometer Initializing Set WL. Set Grating Set exit ZABER Initializing Actuator operating

8. Equipment setting Mono chrometer Confocal Microscope pmt laser signal Command Serial port TCSPC Micro positioning by actuator Setting WL.

9. Flowing chart TCSPC init Zaber initMonocrometer init mode XY scanning WL scanning Single mode Measure pixel # Data processing EXIT START Operate monocrometer Operate actuator

10. Front panel TCSPC control panel Monochrometer Control panel Actuator control panel

11. TCSPC control panel Decay curve display Mode select button Measure start & quit button Current state display

12. Monochrometer & Actuator control panel Pixel # : A number of point of measure Scanning gap : A space between pixels Starting point : Wave length where first pixel Final WL : Wave length where final pixel Current position : reply data from device

13. TCSPC initializing

14. Monochrometer initializing

15. Idle state

16. Single mode

17. Scan XY mode

18. Scan WL. mode

19. Actuator closing

20. Test result Wave length scanning mode Single measure mode