1. Nucleic Acid Engineering Name of Members

2. Introduction Contents Conclusion Comments or Suggestions References

3. Introduction Angew. Chem. Int. Ed. 47, 6259-6262 (2008) Mol. Pharm. 2, 194-205 (2005) Org. Lett. 7, 2085-2088 (2005) MRI imaging Drug delivery system Catalysis with magnetic separation

4. Magnetic Nanoparticles 2AH + H 2 O 2 2A + 2H 2 O Fe 3 O 4 (AH = the colorimetric substrate, which is a hydrogen donor) : TMB (tetramethylbenzidine), DAB (diaminobenzidine) and OPD (o-phenylenediamine) Nat. Nanotechnol. (2007) 2, 577-583 TMBDAB OPD  Peroxidase-mimicking activity of magnetic nanoparticles (Fe 3 O 4 ) Peroxidase-catalyzed reaction

5. Magnetic reusability Magnetic reusability of the nanofactory system. (a)Pictures showing that magnetic separation takes place in 30 seconds. (b) Magnetic reusability after iterative cycles of glucose sensing, magnetic capture, and excessive washing using a magnet. MNPs ☞ easy separation from the solution!

6. Conductive nanocomposite Target glucose was successfully detected over a wide range (0.5 ~ 10 mM), which covers the actual range of glucose concentration in human blood, with excellent storage stability of over two months at RT. Conductive nanocomposite entrapping both MNPs and GOx in mesocellular carbon Adv. Funct. Mater., (2011) 21, 2868-2875

7. MNP-based colorimetric immunoassay Synthesis of Antibody-Conjugated MNPs (MNP-Abs) TEM image of MNPs

8. Conclusion  We have developed MNPs-based biosensor to achieve nano-scale colorimetric or electrochemical biosensing.  We successfully demonstrated that rotaviruses and breast cancer cells could be reliably identified and further quantified through MNPs- promoted colorimetric reaction.  A new synergistically integrated nanocomposite consisting of MNPs and Pt NPs, simultaneously entrapped in mesoporous carbon, is developed as a promising enzyme mimetic candidate to achieve ultrafast colorimetric immunoassays.  This study should accelerate and widen the utility of MNPs as next- generation alternatives to peroxidases.

9. Comments or Suggestions  We have developed MNPs-based biosensor to achieve nano-scale colorimetric or electrochemical biosensing.  We successfully demonstrated that rotaviruses and breast cancer cells could be reliably identified and further quantified through MNPs- promoted colorimetric reaction.  A new synergistically integrated nanocomposite consisting of MNPs and Pt NPs, simultaneously entrapped in mesoporous carbon, is developed as a promising enzyme mimetic candidate to achieve ultrafast colorimetric immunoassays.  This study should accelerate and widen the utility of MNPs as next- generation alternatives to peroxidases.

10. References 1.Ki Soo Park, Moon Il Kim, Dae-Yeon Cho and Hyun Gyu Park*, “Label-free colorimetric detection of nucleic acids based on target- induced shielding action against the peroxidase mimicking activity of magnetic nanoparticles”, Small, 7(11), 1521-1525 (2011) Highlighted as a front cover article 2.Moon Il Kim, Youngjin Ye, Byoung Yeon Won, Sujeong Shin, Jinwoo Lee* and Hyun Gyu Park*, “A highly efficient electrochemical biosensing platform by employing conductive nanocomposite entrapping magnetic nanoparticles and oxidase in mesoporous carbon foam”, Advanced Functional Materials, 21(15), 2868-2875 (2011) 3.Moon Il Kim, Jongmin Shim, Taihua Li, Jinwoo Lee* and Hyun Gyu Park*, “Fabrication of nanoporous nanocomposites entrapping Fe3O4 magnetic nanoparticles and oxidases for colorimetric biosensing”, Chemistry- A European Journal, 17(38), 10700-10707 (2011) 4.Moon Il Kim, Jongmin Shim, Taihua Li, Min-Ah Woo, Daeyeon Cho, Jinwoo Lee and Hyun Gyu Park*, “Colorimetric quantification of galactose using a nanostructured multi-catalyst system entrapping galactose oxidase and magnetic nanoparticles as peroxidase mimetics”, Analyst, 137(5), 1137-1143 (2012) 5.Moon Il Kim, Jongmin Shim, Harshala Parab, Sung Chul Shin, Jinwoo Lee, and Hyun Gyu Park*, "A convenient alcohol sensor using one-pot nanocomposite entrapping alcohol oxidase and magnetic nanoparticles as peroxidase mimetics“, Journal of Nanoscience and Nanotechnology, 12(7), 5914-5919 (2012) 6.Young-Chul Lee†, Moon Il Kim†, Min-Ah Woo, Hyun Gyu Park* and Jong-In Han*, "Effective peroxidase-like activity of a water- solubilized Fe-aminoclay for use in immunoassay“, Biosensors and Bioelectronics, 42, 373-378 (2013.04.15) ISSN: 0956-5663 († these authors equally contributed) 7.Moon Il Kim, Youngjin Ye, Min-Ah Woo, Jinwoo Lee,* and Hyun Gyu Park*, “A Highly Efficient Colorimetric Immunoassay Using a Nanocomposite Entrapping Magnetic and Platinum Nanoparticles in Ordered Mesoporous Carbon”, Advanced Healthcare Materials (in revision) 8.Min-Ah Woo, Moon Il Kim, Jae Hwan Jung, Ki Soo Park, Tae Seok Seo, and Hyun Gyu Park*, “A Novel Colorimetric Immunoassay Utilizing Peroxidase Mimicking Activity of Magnetic Nanoparticles”, International Journal of Molecular Science (submitted)