1. Corynebacteria C. pseudotuberculosis (C
Corynebacteria C.pseudotuberculosis (C.ovis) Caseus lymphadenitis (sheep,goat) ulcerative lymphangitis , acne (horse,cattle) Rhodococcus equi (C.equi) Suppurative bronchopneumonia (Foal , 2-4 month) C.renale group ( C.renale) (C.pilosum) pylonephritis, cystitis (cattle) (C.cestidis) infection from prepuce of male Actinomyces pyogens (C.pyogens) summer mastitis All are pyogenic infectios with capsulated abscess Specimens : pus or L.N. after slaughter 2-pus from skin or lymph vessels 3-bronchial wash with saline 4-Urine under aseptic condition Microscopic Exam. Gram +Ve , pleomorphic( bacilli, short bacilli , cocci), chinese letter. Non motile,non spore non capsulated Direct microscopy or from culture(B.A.)
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Caseous lymphadenitis

3. Corynebacterium diphtheriae
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Corynebacterium diphtheriae
Stained with Epstein Methylene blue Gram iodine stain show chinese letters
Zagazing University Fac. Of Vet. Med. Dep. Of Microbiology

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Pathogenesis C.pseudotuberculosis hemoltic toxin (phospholipase D) Rhodococcus equi (phospholipase C) Action inhibit phagocytosis Rhodococcus equi predisposing factor (decline of maternal imunity at about 6 week) suppurative bronchopneumonia swallow sputum ulcerative colitis and mesentric lymphadenitis

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Culture characters pus specimen Urine(turbid or with precipitate) B.A.(dew drop like colony) Trachial wash ( beta hemolytic) colony ml broth (I.P.) orchitis, abscess(2-6 days) (Strauss reaction) pus B.A.(37C,24h.) Dew drop colony chinese letter like +Ve in C.pseudotuberculosis
Gram
stain
37C,24h.
Guinea pig
male
turbidity
Gram
stain

6. Laboratory diagnosis 1- Microscopy fix film from culture (Gram stain) 2- Culture B.A Strauss reaction in case of suspected C.pseudotuberculosis CAMP C.pseudotuberculosis inhibition Rhodococcus equi enhancement C.renale enhancement Biochemical tests (nitrate, urease, sugar fermen.) Differential diagnosis Ulcerative lymphangitis Cutaneous farcy (Glanders) Epizootic lymphangitis
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7. Don’t let the streak lines touch
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CAMP Test
Rhodococcus
equi
S.aureus
C.Pseudo
tuberculosis
Don’t let the streak lines touch

8. Differential diagnosis
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Differential diagnosis
Disease in
horse
lesions
Cause
Morph
-ology
culture
Strauss
reaction
Mallein
test
Ulcerative
lymphangitis
Ulcer. Inf.
Of lymph
Vessels
(limbs)
C.pseudoTuber.
Gr+ve
Chinese
Letter like
B.A. or
Lofferes
serum
+Ve
-Ve
Glanders
Farcy buds on
Lymph
Vessels +
Pulmonary
infections
Actinobacillus
mallei
Gr –ve
bacilli
Potato
Medium
Yellow or
Orange
colony
Epizootic
Vessel
Infl.
Histoplasma
farciminosum
Gr -Ve
Yeast
like
Growth
After
1-3 week

9. foal died with pneumonia 2-4 month, lung specimen turned to bacteriology laboratory, the isolated colony was.
Macroscopic: small-medium mucoid, round colonies; no hemolysis
***Note characteristic salmon-pink color***
Gram stain Gram positive rods- note that the organisms are quite pleomorphic, and many look almost coccoid
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This isolate was Rhodococcus equi

10. Pus from a chronic infected horse leg was turned to bact. Lab
Pus from a chronic infected horse leg was turned to bact. Lab. 1-Enumerate name of suspected bacterial isolates 2-Cofirmatory laboratory differentiaton between them 3-If it was Gr+ve pleomorphic, differentiate C.pseudotuberculosis and rhodococcus equi. Urine sample with pus sediments turned to your laboratory from a cow with arched back and colicy pain , 1- Enumerate bacterial causative isolates and their culture media . 2- Laboratory confirmation of one of them. 3- after staining , if the m.o. was Gr+ve , chinese letter like differentiate it from other pleomorphic types Foal with bronchopneumonia fever, after a history of similar History
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11. In case of ulcerative lymphangitis in horse usually lymph nodes are affected . a-fore legs l.n b-hind legs l.n c-neck l.n d-chest, l.n [97]lymph nodes when infected with C-pseudotuberculosis in sheep. Look like. a-Onion like. b-Pear like. c-Nodular like. d-None of all. [98]Strauss reaction could be done in a-in rabbite .b-female guinea pig c-in male guinea pig . d-in rat. [99]Strauss reaction characterised by. a-nodules in inguinal lymph nodes. b-orchitis & pus formation in the testis. c-lymphangitis of inguinal lymph vessels. d- lympha- denitis of inguinal lymph nodes. [100]Coryne.pseudotuberculosis “C.ovis” causes The following In horse. a-caseus lymphadenitis . b-ulcerative lymphangitis. c-epizootic lymphangitis. D-glanders .

12. Questions Advantages of performing a culture?
On which ones would you perform susceptibility testing?
M.O antibiotic growth around the antibiotic( sensitive
m.o. to the antibiotic)
no growth around the antibiotic leaving
inhibition zone(resistant m.o. to the
antibiotic)

13. S.aureus

14. For today’s lab zutalaba.com All Gram positive organisms
Look at your cultures from Tuesday’s broth - do catalase on these
For Gram positive cocci, do catalase
positives: do coagulase AND mannitol salt (can split the plate)
negatives: IF MILK SAMPLE: do CAMP
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