1. Connective Tissues of the Skeleton JACKI HOUGHTON, DC Cartilages Bones

2. Functions of the Skeletal System A. Support B. Protection C. Movement D. Mineral Storage (Calcium + Phosphorus) E. Hematopoiesis (blood cell formation in red marrow) F. Energy Storage (lipids/fat stored in yellow marrow)

3. Axial vs Appendicular

4. Bones Functions Support (give body it’s shape) Movement: muscles attach by tendons and use bones as levers to move body Protection Skull – brain Vertebrae – spinal cord Rib cage – thoracic organs Mineral storage Calcium and phosphorus Released as ions into blood as needed Blood cell formation and energy storage Bone marrow: red makes blood, yellow stores fat

5. Chemical composition of bones Cells, matrix of collagen fibers and ground substance (organic: 35%) Contribute to the flexibility and tensile strength Mineral crystals (inorganic: 65%) Primarily calcium phosphate interacting with calcium hydroxide to form crystals of hydroxyapatite Lie in and around the collagen fibrils in extracellular matrix Contribute to bone hardness Small amount of water

6. Microscopic anatomy Bone organization the osteon Concentric lamellae Interstitial lamellae Circumferential lamellae Blood Supply Central canal, Volkman’s canals, canaliculi Trabeculae Architecture of weight-bearing bones

7. Terms (examples) chondro refers to cartilage chondrocyte endochondral perichondrium osteo refers to bone osteogenesis osteocyte periostium blast refers to precursor cell or one that produces something osteoblast cyte refers to cell osteocyte

8. cells Osteoprogenitor cells Stem cells, precursor to osteoblasts Present in inner periosteum and endosteum Osteocytes Mature bone cells forming from osteoblasts Live in lacunae Maintain area around them Produce collagen Osteoblasts Lay down osteoid -collagen Osteoclasts Giant, multinucleated, mobile. Derived from white blood cell Secretes HCl, breaks down calcium and phosphate. Lysomal enzymes breakes down collagen. osteolysis

9. Nutrients diffuse from vessels in central canal Alternating direction of collagen fibers increases resistance to twisting forces http://youtu.be/X6E5Rz9tOKE Isolated osteon: http://youtu.be/HUdwCvHZguM

10. Membranes Periosteum Covers outer surface of bone except ends. Double membrane Inner: osteoprogenitor cells, osteoblasts & osteoclasts for remodeling Outer: tough, fibrous, attaches bone to connective tissue with “Sharpy’s Fibers” Endosteum contains osteoprogenitor cells lines marrow cavity

11. Periosteum Periosteal Bud - A vascular connective tissue bud from the perichondrium that enters the cartilage of a developing long bone and contributes to the formation of a center for ossification.perichondriumcartilageossification Connective tissue membrane Covers entire outer surface of bone except at epiphyses Two sublayers 1. Outer fibrous layer of dense irregular connective tissue 2. Inner (deep) cellular osteogenic layer on the compact bone containing osteoprogenitor cells (stem cells that give rise to osteoblasts) Osteoblasts: bone depositing cells Also osteoclasts: bone destroying cells (from the white blood cell line) Secured to bone by perforating fibers (Sharpey’s fibers) Endosteum Covers the internal bone surfaces Is also osteogenic

12. Classification of bones by shape Long bones Short bones Flat bones Irregular bones Pneumatized bones Sesamoid bones (Short bones include sesmoid bones)

13. Know these!

14. Gross anatomy of bones Compact bone Typical long bone Epiphysis Diaphysis Metaphysis Medullary cavity Articular cartilage Epiphyseal plates Periosteum Sharpey’s fibers Spongy (trabecular) bone Blood vessels Medullary cavity Membranes Periosteum Endosteum

15. Flat bones Spongy bone is called diploe when its in flat bones Have bone marrow but no marrow cavity

16. Bone development Osteogenesis: “formation of bone” From osteoblasts Bone tissue first appears in week 8 (embryo) Ossification: “to turn into bone” Intramembranous ossification (also called “dermal” since occurs deep in dermis): forms directly from mesenchyme (not modeled first in cartilage) Most skull bones except a few at base Clavicles (collar bones) Sesamoid bones (like the patella) Endochondral ossification: modeled in hyaline cartilage then replaced by bone tissue All the rest of the bones

17. Cartilage Embryo More prevalent in the embryo than in adult Skeleton is initially mostly cartilage Bone replaces cartilage in fetal and childhood periods 3 types: hyaline, elastic and fibrocartilage So what is cartilage? It is a connective tissue which has differing properties, depending on it’s function. Hyaline cartilage lines the ends of bones and cushions them. The hyaline wears better than bone. Elastic cartilage is still but will bend and return to it’s original shape. Fibrocartilage has great tensile strength and can absorb shock.

18. Endochondral ossification Stages 1-3 during fetal week 9 through 9 th month Stage 4 is just before birth Stage 5 is process of long bone growth during childhood & adolescence

19. Endochondral ossification Modeled in hyaline cartilage, called cartilage model Perichondrium is invaded by vessels and becomes periosteum Osteoblasts in periosteum lay down collar of bone around diaphysis Calcification in center of diaphysis Primary ossification centers Secondary ossification in epiphyses Epiphyseal growth plates close at end of adolescence Diaphysis and epiphysis fuse No more bone lengthening See next slide

20. Remember the three germ tissues… 1. Ectoderm - epithelial 2. Endoderm - epithelial 3. Mesoderm is a mesenchyme tissue Mesenchyme cells are star shaped and do not attach to one another, therefore migrate freely From the last slide: Intramembranous ossification: forms directly from mesenchyme (not modeled first in cartilage) Most skull bones except a few at base Clavicles (collar bones) Sesmoid bones (like the patella)

21. Intramembranous ossification (osteoid is the organic part)

22. Epiphyseal growth plates in child, left, and lines in adult, right (see arrows)

23. Factors regulating bone growth Vitamin D: increases calcium from gut Parathyroid hormone (PTH): increases blood calcium (some of this comes out of bone) Calcitonin: decreases blood calcium (opposes PTH) Growth hormone & thyroid hormone: modulate bone growth Sex hormones: growth spurt at adolescense and closure of epiphyses

24. Compact bone Osteons: pillars Lamellae: concentric tubes Haversian canals Osteocytes

25. Spongy bone Layers of lamellae and osteocytes Seem to align along stress lines Spongy bone is made up of a network of bony spicules arranged into parallel struts or thick, branching plates called trabeculae

26. Bone remodeling Osteoclasts Bone resorption Osteoblasts Bone deposition Triggers Hormonal: parathyroid hormone Mechanical stress Osteocytes are transformed osteoblasts

27. Repair of bone fractures (breaks) Simple and compound fractures Closed and open reduction

28. Disorders of cartilage and bone Defective collagen Numerous genetic disorders eg. Osteogenesis imperfecta (brittle bones) – AD (autosomal dominant) eg. Ehlers-Danlos (rubber man) Defective endochondral ossification eg. Achondroplasia (short –limb dwarfism) - AD Inadequate calcification (requires calcium and vitamin D) Osteomalacia (soft bones) in adults Rickets in children Note: “AD” here means autosomal dominant inheritance

29. (continued) Pagets disease – excessive turnover, abnormal bone Osteosarcoma – bone cancer, affecting children primarily Osteoporosis – usually age related, esp. females Low bone mass and increased fractures Resorption outpaces bone deposition

30. Normal bone Osteoporotic bone

31. Bone markings reflect the stresses

32. Bone markings *Bone pain is called ostealgia* Projections that are the attachments sites for muscles and ligaments Surfaces that form joints Depressions and openings Learn them using: Marieb lab book p 101, Table 8.1, Bone Markings or Martini p 128, Table 5.1, Common Bone Marking Terminology (next slide)

33. Martini p 128, Table 5.1, Common Bone Marking Terminology (for figure see next slide)