1. Study Guide

2. Most common, specimen is darker than background Bright Field Used to see bacteria from syphilis Used for viewing live unstained specimens Dark field Used for viewing intracellular structures Contrast uses fluorescent dyes and a UV light Used for indentifying tuberculosis Fluorescence

3. Uses only one type of stain Simple Uses a primary stain and a counter stain Gram Stain Acid Fast Stain Differential Capsule & flagellar stain Special

4. Produce a culture Sample is placed in a container of sterile medium Inoculation Incubator used to adjust for proper conditions for growth of a sample Incubation Separates one sample from another Isolation Culture observed w/o microscope for growth Inspection Determine type of microbe to level of species Identification