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BIO409/509 Cell and Molecular Biology
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SECOND Methods paper assignment due Wed., 4/20 (you don’t do this assignment if you are in the 4H STEM Ambassador Program) Assignment: Choose a SECOND method in Molecular Biology or Cell Biology and write 2-3 page paper about the following: 1)Describe the steps done in the method 2)Explain the “highlight” steps: What are the key “tricks” in the method. 3)Describe two different applications of the method to help demonstrate how the method is valuable. On an extra page, include a bibliography with any resources you used: Our textbook, web sites, personal communications, journal articles, etc.
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BIO409/509 Cell and Molecular Biology We will skip Chapter 7 DNA Replication and never cover it. Finish Chapter 8 RNA Synthesis on on to Chapter 9 Protein Synthesis. That’s the end of Part II. We’ll start talking about Chapter 10 The Nucleus this week.
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RNA Synthesis and Processing 8
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Figure 8.31 Histone acetylation (Part 2)
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Figure 8.37 DNA methylation
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Figure 8.38 Maintenance of methylation patterns
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Each step in gene expression has the potential for being regulated if it is the rate limiting step.
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Regulation of Protein Function Cells can regulate the amounts and the activities of their proteins. Three mechanisms: Regulation by small molecules Phosphorylation and other modifications Protein-protein interactions
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Figure 8.44 Processing of eukaryotic messenger RNAs (Part 1)
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Figure 8.47 Splicing of pre-mRNA
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Figure 8.48 Assembly of the spliceosome (Part 2)
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Figure 8.52 Alternative splicing in Drosophila sex determination
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Figure 8.55 mRNA degradation
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Protein Synthesis, Processing, and Regulation 9
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9 Protein Synthesis, Processing, and Regulation Translation of mRNA Protein Folding and Processing Regulation of Protein Function Protein Degradation
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Figure 9.9 Overview of translation
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Figure 9.7 Prokaryotic and eukaryotic mRNAs
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Figure 9.8 Signals for translation initiation
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Table 9.1 Translation Factors
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Figure 9.11 Initiation of translation in eukaryotic cells (Part 1)
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Figure 9.17 Translational regulation of ferritin
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Figure 9.22 Regulation of eIF4E
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Translation of mRNA RNA interference is mediated by: Small interfering RNAs (siRNAs)— produced from double-stranded RNAs by the nuclease Dicer. MicroRNAs (miRNAs)—transcribed by RNA polymerase II, then cleaved by nucleases Drosha and Dicer.
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Figure 9.13 Elongation stage of translation (Part 1)
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Figure 9.13 Elongation stage of translation (Part 3)
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