1. Do we have the right to perform experiments on animals? Barbara Malinowska Department of Experimental Physiology and Pathophysiology Medical University of Białystok Poland

2. father of experimental physiology Galen of Pergamon “Vivisectionists, who use living animals for their experiments, certainly act cruelly, although their aim is praiseworthy, and they can justify their cruelty, since animals must be regarded as man’s instruments; Franco N.: Animal experiments in biomedical research: A historical perspective. Animals 2013,3,238. 1846 ether R. Koch

4. Estonia – 81% Denmark – 78% Lithuania – 78% Luxemburg - 32% Switzerland – 30% Poland – 69%; 13% Germany – 61%; 23%

5. Animal rights activists NO!  it causes suffering to animals;  the benefits to human beings are not proven;  experiments violate the rights of animals;  any benefits to human beings that animal testing does provide could be produced in other ways;  animals surely deserve to live their lives.

6. YES! ANIMAL RESEARCH SAVES LIVES SUFFERING DURING EXPERIMENTS IS MINIMIZED!!!

7. Veterinary medicine and animal testing ANIMAL RESEARCH HELPS ANIMALS TOO

8. human pig heart mice and men are around 99% genetically similar

9. Animals are used to:  understand how diseases affect living tissue  develop and test treatments for human and animals  train future health-care professionals

10. RESULTS OF EXPERIMENTS ON ANIMALS

11. TREATMENT FOR RABIES (DOGS, RABBITS);

12. –treatment for RICKETS (dogs); LEPROSY (monkeys, armadillos); etc. –prevention of DIPHTHERIA (horses); POLIO (rabbits); RUBELLA (monkeys) RICKETS LEPROSY DIPHTHERIA POLIO RUBELLA

13. –discovery of INSULIN (dogs); MODERN ANAESTHESIA (dogs); DNA (mice & rats) –development of laparoscopic surgical techniques (pigs); open heart surgery (dogs);

14. Paralysed man walks after spinal research breakthrough Prof. Geoffrey RaismanDr. Paweł TabakowDarek Fidyk 2014 Repair of spinal cord injury by transplantation of olfactory ensheathing cells.

15. May-Britt Moser, Edvard Moser, and their rats that they use in their groundbreaking neuroscience research. 2014 Franco N.: Animal experiments in biomedical research: A historical perspective. Animals 2013,3,238. NOBEL PRIZES Of the 103 NOBEL PRIZES in physiology or medicine given since 1901, on 83 occasions work conducted on vetrabrate species (other than human) was awarded

16. EDUCATION

17. Virtual frog and virtual scalpel animal section on-line

18. Good science and good animal care go hand-in-hand  Good science requires a good animal care.  Animals that are in poor condition, discomfort or pain are poor research subjects.  Animal well-being supports the integrity of the research.

19. They see a rat in a cage We seek a cure for diseases Kehinde EO, Med Princ Pract. 2013;22 Suppl 1:52-61 clear regulations and policies Only clear regulations and policies can reconcile both sides and help to ensure the human treatment of animals

20. REGULATIONS OF ANIMAL EXPERIMENTATION Animal Experiments Directive 2010/63/EU on the protection of animals used for scientific purposes

21. „It is essential, both on moral and scientific grounds, to ensure that each use of an animal is carefully evalueated as to the scientific or educational validity, usefulness and relevance of the expected results of that use” „The likely harm to the animal should be balanced against the expected benefits of the project”

22. National Ethics Committee for Experiments on Animals Local Ethics Committees for Experiments on Animals

23. Number of members of Ethics Committee for Experiments on Animals nationallocal Total1512 Scientist (biologists, medical, veterinary doctors, pharmacists etc.) 9 (60%)6 (50%) Humanists (ethicists, philosophers, lawyers etc.)3 (20%)3 (25%) Members of organizations connecting with animal protecion 3 (20%)3 (25%) YES! 2005: 51%  YES 2015: 2/3  YES

24. Rex Leonard Burch. The 3 R’s principles William Moy Stratton Russell and 1959 Replacement Reduction Refinement

25.  computer-based systems & mathematical modelling;  cell, tissue and organ cultures;  use of lower organisms (bacteria and fungi)  physico-chemical techniques; e.g., the commercial test system EYTEX can predict whether a chemical will irritate the eyes, Replacement

26. Minimizing the number of animals used in experiments by:  improving experimental and data analysis techniques;  information (to reduce unnecessary duplication of animal work);  performing pilot studies to determine some of the potential problems in an experiment before numerous animals are used;  gathering a maximum amount of information from each animal,  using the appropriate species of animal so that useful data are collected Reduction

27.  improving animal welfare in laboratories;  refining experimental protocols to minimize pain or distress whenever possible. Refinement

28. Local Ethics Committees for Experiments on Animals 1.determination of the level of severity; 2.consideration of humane endpoints; 3.determination of the indispensability of the experimental proposal; 4.classification into applied or basic research; 5.determination of the probability of success 6.cost-benefit analysis

29. 1. Determination of the level of severity  non-recovery (anaesthesia);  mild (short-term mild pain)  moderate;  severe

30. It can be defined as the point at which an experimental animal's pain and/or distress is terminated, minimized or reduced, by taking action such as euthanasia the animal humanely, terminating a painful procedure, or giving treatment to relieve pain and/or distress. A human endpoint

31. There are no alternative ways! Determination of the indispensability of the experimental proposal

32. Classification into applied or basic research

33. Rollin B.: Animal research: a moral science. EMBO reports, 2007;8:521 5. Determination of the probability of success. 6. Cost-benefit analysis - pain, - suffering - distress Potential benefits of experiments on animals to humans, other animals and enviroment

34. Local Ethics Committees for Experiments on Animals YES! Experiments on animals Non-technical report for the general public Experiments on animals

35. 1.Using animals for research activities is a privilege, not (and) a right! 2.It is important to keep focusing on the continuous improvement of the wellbeing of laboratory animals, as well as further development of replacement alternatives and regulations for animal experiments.

36. THANK YOU FOR YOUR ATTENTION!