Presentation is loading. Please wait.

Presentation is loading. Please wait.

Polymerase Chain Reaction (PCR): DNA Replication in vitro DNA replication in vivo DNA replication in vivo –Occurs in S-phase –Helicase, primers, DNA polymerase.

Published byMoris Jacob Tate Modified over 8 years ago

Similar presentations

Presentation on theme: "Polymerase Chain Reaction (PCR): DNA Replication in vitro DNA replication in vivo DNA replication in vivo –Occurs in S-phase –Helicase, primers, DNA polymerase."— Presentation transcript:

1 Polymerase Chain Reaction (PCR): DNA Replication in vitro DNA replication in vivo DNA replication in vivo –Occurs in S-phase –Helicase, primers, DNA polymerase PCR PCR –Replicates portions of DNA specified by primers –Thermal cycler, primers, DNA (Taq) polymerase, ATCG –Uses heating cycles to control DNA replication in vitro –2 n ; n = number of cycles –2, 4, 8, 16, 32, 64, 128, 256, 512, 1024, 2048, 4096...

2 Polymerase Chain Reaction (PCR): Applications Why: Why: –Forensics, Research, Paternity test, Medical screening When: When: –Small DNA sample –Check for presence of a gene location Must be used in conjunction with Electrophoresis Must be used in conjunction with Electrophoresis

3 PCR: What happens? Ingredients: Sample DNA, DNA (Taq) polymerase, primers, ATCG; Thermal cycler controls temperature. Ingredients: Sample DNA, DNA (Taq) polymerase, primers, ATCG; Thermal cycler controls temperature. Cooling allows primers to anneal Heating separates DNA strands DNA polymerase elongates new DNA chain Heat separates strands Primers anneal New chain is built REPEAT..

Download ppt "Polymerase Chain Reaction (PCR): DNA Replication in vitro DNA replication in vivo DNA replication in vivo –Occurs in S-phase –Helicase, primers, DNA polymerase."

Similar presentations

Structure of DNA. Polymerase Chain Reaction - PCR PCR amplifies DNA –Makes lots and lots of copies of a few copies of DNA –Can copy different lengths.

Structure of DNA. Polymerase Chain Reaction - PCR PCR amplifies DNA –Makes lots and lots of copies of a few copies of DNA –Can copy different lengths.
Polymerase Chain Reaction (PCR) and its Applications.

Polymerase Chain Reaction (PCR) and its Applications.
PCR way of copying specific DNA fragments from small sample DNA material molecular photocopying It’s fast, inexpensive and simple Polymerase Chain Reaction.

PCR way of copying specific DNA fragments from small sample DNA material "molecular photocopying" It’s fast, inexpensive and simple Polymerase Chain Reaction.
DNA Fingerprinting and Forensic Analysis Chapter 8.

DNA Fingerprinting and Forensic Analysis Chapter 8.
PCR Overview Lifted from various powerpoint presentations on the internet.

PCR Overview Lifted from various powerpoint presentations on the internet.
Definition of PCR Requirements for PCR PCR Process Agarose gel electrophoresis.

Definition of PCR Requirements for PCR PCR Process Agarose gel electrophoresis.
General Genetics. PCR 1.Introduce the students to the preparation of the PCR reaction. PCR 2.Examine the PCR products on agarose gel electrophoresis.

General Genetics. PCR 1.Introduce the students to the preparation of the PCR reaction. PCR 2.Examine the PCR products on agarose gel electrophoresis.
PCR – Polymerase chain reaction

PCR – Polymerase chain reaction
The PCR The Polymerase Chain Reaction. The PCR is used to make copies of DNA (amplification). Whole genome OR DNA fragments.

The PCR The Polymerase Chain Reaction. The PCR is used to make copies of DNA (amplification). Whole genome OR DNA fragments.
The polymerase chain reaction (PCR) rapidly

The polymerase chain reaction (PCR) rapidly
POLYMERASE CHAIN REACTION (PCR). THE TEMPLATE DNA GOES THROUGH A CYCLE OF HEATING AND COOLING TO FORM A NEW STRAND OF DNA THAT ONLY COMPRISES OF THE CANCEROUS.

POLYMERASE CHAIN REACTION (PCR). THE TEMPLATE DNA GOES THROUGH A CYCLE OF HEATING AND COOLING TO FORM A NEW STRAND OF DNA THAT ONLY COMPRISES OF THE CANCEROUS.
Polymerase Chain Reaction (PCR) is when you amplify the number of copies of a specific region of DNA, in order to produce enough DNA it be adequately.

Polymerase Chain Reaction (PCR) is when you amplify the number of copies of a specific region of DNA, in order to produce enough DNA it be adequately.
Polymerase Chain Reaction (PCR) and its Applications.

Polymerase Chain Reaction (PCR) and its Applications.
Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction (PCR)
Let’s Review Biotechnology! (Why not?). Embryonic Cloning Label parts and steps. + ?

Let’s Review Biotechnology! (Why not?). Embryonic Cloning Label parts and steps. + ?
Recombinant DNA Technology………..

Recombinant DNA Technology………..
Polymerase Chain Reaction PCR. invented by Karry B. Mullis (1983, Nobel Prize 1993) patent sold by Cetus corp. to La Roche for $300 million depends on.

Polymerase Chain Reaction PCR. invented by Karry B. Mullis (1983, Nobel Prize 1993) patent sold by Cetus corp. to La Roche for $300 million depends on.
Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction (PCR)
Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction (PCR)
Qai Gordon and Maddy Marchetti. What is Polymerase Chain Reaction? Polymerase Chain Reaction ( PCR ) is a process that amplifies small pieces of DNA to.

Qai Gordon and Maddy Marchetti. What is Polymerase Chain Reaction? Polymerase Chain Reaction ( PCR ) is a process that amplifies small pieces of DNA to.

Similar presentations

Ads by Google