1. Sofia Aires Martins Jornadas Pedagógicas do Técnico Ensino 2025 March, 2016

2. About us.... (me ?) 19711981 1983 1989 1992 Animal production course !

3. 1994 Biotechnology Engineering 1999 MsC in Biochemical Engineering 2003 PhD Biotechnology 2008 Nanotechnology 2014 Magnomics! OK, let’s start all over again…

4. Who are we ? Magnomics SA is a portuguese start-up commited to deliver rapid, point-of-care (POC) molecular diagnostic tests Founded: June 2014 (2013) First investment round: June, 2014

5. INESC-MN INSTITUTIONAL SUPPORT Filipe Cardoso Sofia Martins José Germano Verónica Romão PhysicsBiotechElectronicsBiotech João Pereira Business The team…

6. BOARD & ADVISORS Miguel Botto Board Member Investment Director at Portugal Ventures, over 20 years as a VC Santiago Salazar Board Member Partner at Busy Angels, over 15 years experience as CEO of multinationals Ronan Molloy Board Member 20 years executive experience in both human and animal diagnostics Sheila Andrew Advisor Bovine mastitis expert (University of Connecticut), former NMC president George Saperstein Advisor Bovine mastitis expert (Tufts University, retired), former president & founder of Lotek Bernard Mandrand Advisor Diagnostics expert, former head of R&D at BioMérieux 6 Board and advisors…

7. The technology... Just after 2008 Electronic reader Portable biochip

8. Platform Features Electronic board: chip control and read-out Surface chemistry: bioreceptor immobilization Sensing element: MR sensor Microfluidic system: sample handling Proteins DNA Small molecules

9. Berlin Meeting for EU projects…. ALUMAGNA MultiFollowUP Early cancer diagnosis through genetic/protein markers The beginning ….

10. Bio-Teams: from the laboratory to the market Ana Margarida Dias Susana Palma Gabriel Mendes Pedro Pinto

12. A good idea….. An excellent team… PoC for early cancer detection !!!

14. Investors Money

16. Why should we invest in you? How much money do you need? How are you going to spend it ?

19. COHITEC Program Turning science into business 5 month training program

20. Bovine mastitis problem: Mastitis is an infection of the mammary gland Multitude of pathogens Hard treatment (chronic disease), dependent upon the pathogen Global losses: €7. 7 Billion year Antibiotic burden Mastitis diagnostics (2011): 200 M€ revenues (2016: 300 M€)

21. Our solution: On-farm solution for pathogen identification Several pathogens are detected at the same time Fast results Sensitive No requirements for specialized personnel

22. COMPETITION Use in farms Time Added Value Pathogen/Resistance Identification Yes1-2h+++Yes 22 No 48-72h (+ Logistics) -Yes Culture No 1-2h (+ Logistics) -Yes Molecular Yes<1h+No Existing on-site

24. At FMV….. June, 2014

25. Let’s build a product… Miniaturized sample treatment unit Milk Miniaturized PCR Miniaturized DNA detection system 2 years for first prototype ~ 0.5 M€

27. Sample treatment module Sample: Milk Target concentration: 10 3 cells/mL 1st approach: Direct amplification from milk samples

28. 2nd approach: Extract DNA from bacteria present in milk samples

29. …miniaturize… Manual version: V0

30. 10 2 10 3 10 4 10 5 10 6 cells/mL Positive control (commercial Kit) 10 6 10 5 10 4 10 3 10 2 0 0 S. aureus 10 2 10 3 10 4 10 5 0 10 6 cells/mL S. uberis 10 2 10 3 10 4 10 5 10 6 0 cells/mL

31. Automated version: V1 July 2015

32. Automated version: V2 The Millennium Falcon….

33. The PCR module… Sub-micron liter volume 35 cycles in less than 20 minutes Version: V1, January 2015

34. S. uberisS. sureusblaZ16S rRNA However….

35. New solution… 10 µL volume 35 cycles, 60 min The toaster…

36. The detection module… Multiplex detection up to 14 different analytes Nucelic acids, proteins, others) Portable Autonomy ≥ 8 h Analysis time ≤ 40 min Affordable

37. DNA extracts…

38. Next steps… Integration of all modules into one single cartridge Automation Optimization Industrialize

40. Nanolab, January 2016 Thank you!